Improved enantioselectivity of thermostable esterase ST0071 from archaeon Sulfolobus tokodaii by site-saturation mutagenesis

Masanaru Ozaki; Norifumi Kawakami; Hiromichi Ohta; Kenji Miyamoto

doi:10.1080/10242422.2016.1247823

Improved enantioselectivity of thermostable esterase ST0071 from archaeon Sulfolobus tokodaii by site-saturation mutagenesis

Masanaru Ozaki, Norifumi Kawakami, Hiromichi Ohta, Kenji Miyamoto

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

An archaeon GGG(A)X-type esterase (ST0071) can catalyze the hydrolysis of various acetates of secondary alcohols, but shows low enantioselectivity. Using structure-guided site-saturation mutagenesis, we successfully identified a G274W variant that has excellent selectivity compared with that of wild-type ST0071.

Original language	English
Pages (from-to)	249-252
Number of pages	4
Journal	Biocatalysis and Biotransformation
Volume	34
Issue number	5
DOIs	https://doi.org/10.1080/10242422.2016.1247823
Publication status	Published - 2016 Sept 2

Keywords

Sulfolobus tokodaii
directed evolution
enantioselectivity
esterase

ASJC Scopus subject areas

Biotechnology
Catalysis
Biochemistry

Access to Document

10.1080/10242422.2016.1247823

Cite this

@article{aa24f37bbbb84373ab5f71da3b56e851,

title = "Improved enantioselectivity of thermostable esterase ST0071 from archaeon Sulfolobus tokodaii by site-saturation mutagenesis",

abstract = "An archaeon GGG(A)X-type esterase (ST0071) can catalyze the hydrolysis of various acetates of secondary alcohols, but shows low enantioselectivity. Using structure-guided site-saturation mutagenesis, we successfully identified a G274W variant that has excellent selectivity compared with that of wild-type ST0071.",

keywords = "Sulfolobus tokodaii, directed evolution, enantioselectivity, esterase",

author = "Masanaru Ozaki and Norifumi Kawakami and Hiromichi Ohta and Kenji Miyamoto",

year = "2016",

month = sep,

day = "2",

doi = "10.1080/10242422.2016.1247823",

language = "English",

volume = "34",

pages = "249--252",

journal = "Biocatalysis and Biotransformation",

issn = "1024-2422",

publisher = "Informa Healthcare",

number = "5",

}

TY - JOUR

T1 - Improved enantioselectivity of thermostable esterase ST0071 from archaeon Sulfolobus tokodaii by site-saturation mutagenesis

AU - Ozaki, Masanaru

AU - Kawakami, Norifumi

AU - Ohta, Hiromichi

AU - Miyamoto, Kenji

PY - 2016/9/2

Y1 - 2016/9/2

N2 - An archaeon GGG(A)X-type esterase (ST0071) can catalyze the hydrolysis of various acetates of secondary alcohols, but shows low enantioselectivity. Using structure-guided site-saturation mutagenesis, we successfully identified a G274W variant that has excellent selectivity compared with that of wild-type ST0071.

AB - An archaeon GGG(A)X-type esterase (ST0071) can catalyze the hydrolysis of various acetates of secondary alcohols, but shows low enantioselectivity. Using structure-guided site-saturation mutagenesis, we successfully identified a G274W variant that has excellent selectivity compared with that of wild-type ST0071.

KW - Sulfolobus tokodaii

KW - directed evolution

KW - enantioselectivity

KW - esterase

UR - http://www.scopus.com/inward/record.url?scp=85003443738&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85003443738&partnerID=8YFLogxK

U2 - 10.1080/10242422.2016.1247823

DO - 10.1080/10242422.2016.1247823

M3 - Article

AN - SCOPUS:85003443738

SN - 1024-2422

VL - 34

SP - 249

EP - 252

JO - Biocatalysis and Biotransformation

JF - Biocatalysis and Biotransformation

IS - 5

ER -

Improved enantioselectivity of thermostable esterase ST0071 from archaeon Sulfolobus tokodaii by site-saturation mutagenesis

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this