Improved enantioselectivity of thermostable esterase ST0071 from archaeon Sulfolobus tokodaii by site-saturation mutagenesis

Masanaru Ozaki, Norifumi Kawakami, Hiromichi Ohta, Kenji Miyamoto

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

An archaeon GGG(A)X-type esterase (ST0071) can catalyze the hydrolysis of various acetates of secondary alcohols, but shows low enantioselectivity. Using structure-guided site-saturation mutagenesis, we successfully identified a G274W variant that has excellent selectivity compared with that of wild-type ST0071.

Original languageEnglish
Pages (from-to)249-252
Number of pages4
JournalBiocatalysis and Biotransformation
Volume34
Issue number5
DOIs
Publication statusPublished - 2016 Sep 2

Keywords

  • Sulfolobus tokodaii
  • directed evolution
  • enantioselectivity
  • esterase

ASJC Scopus subject areas

  • Biotechnology
  • Catalysis
  • Biochemistry

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