Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates

Kohei Kasai, Yuka Kimura, Shogo Miyata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.

Original languageEnglish
Pages (from-to)354-361
Number of pages8
JournalMaterials Science and Engineering C
Volume78
DOIs
Publication statusPublished - 2017 Sep 1

Fingerprint

feeders
stem cells
Ozone
Stem cells
ozone
mice
adhesion
Feeder Cells
Adhesion
Polystyrenes
Substrates
polystyrene
Cell culture
Cells
coatings
Coatings
Cell adhesion
matrices
surface treatment
medicine

Keywords

  • Embryonic stem cell
  • Feeder-free culture
  • Pluripotent stem cell
  • Surface modification
  • UV/ozone treatment
  • X-ray photoelectron spectroscopy

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates. / Kasai, Kohei; Kimura, Yuka; Miyata, Shogo.

In: Materials Science and Engineering C, Vol. 78, 01.09.2017, p. 354-361.

Research output: Contribution to journalArticle

@article{9ad81138b30e4d5fb70bc037197bcd09,
title = "Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates",
abstract = "Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.",
keywords = "Embryonic stem cell, Feeder-free culture, Pluripotent stem cell, Surface modification, UV/ozone treatment, X-ray photoelectron spectroscopy",
author = "Kohei Kasai and Yuka Kimura and Shogo Miyata",
year = "2017",
month = "9",
day = "1",
doi = "10.1016/j.msec.2017.04.021",
language = "English",
volume = "78",
pages = "354--361",
journal = "Materials Science and Engineering C",
issn = "0928-4931",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates

AU - Kasai, Kohei

AU - Kimura, Yuka

AU - Miyata, Shogo

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.

AB - Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.

KW - Embryonic stem cell

KW - Feeder-free culture

KW - Pluripotent stem cell

KW - Surface modification

KW - UV/ozone treatment

KW - X-ray photoelectron spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85018510108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018510108&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2017.04.021

DO - 10.1016/j.msec.2017.04.021

M3 - Article

C2 - 28575995

AN - SCOPUS:85018510108

VL - 78

SP - 354

EP - 361

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

ER -