Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates

Kohei Kasai; Yuka Kimura; Shogo Miyata

doi:10.1016/j.msec.2017.04.021

Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates

Kohei Kasai, Yuka Kimura, Shogo Miyata

Department of Mechanical Engineering

Research output: Contribution to journal › Article

9 Citations (Scopus)

Abstract

Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.

Original language	English
Pages (from-to)	354-361
Number of pages	8
Journal	Materials Science and Engineering C
Volume	78
DOIs	https://doi.org/10.1016/j.msec.2017.04.021
Publication status	Published - 2017 Sep 1

Keywords

Embryonic stem cell
Feeder-free culture
Pluripotent stem cell
Surface modification
UV/ozone treatment
X-ray photoelectron spectroscopy

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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Kasai, K., Kimura, Y., & Miyata, S. (2017). Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates. Materials Science and Engineering C, 78, 354-361. https://doi.org/10.1016/j.msec.2017.04.021

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