Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates

Kohei Kasai; Yuka Kimura; Shogo Miyata

doi:10.1016/j.msec.2017.04.021

Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates

Kohei Kasai, Yuka Kimura, Shogo Miyata

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.

Original language	English
Pages (from-to)	354-361
Number of pages	8
Journal	Materials Science and Engineering C
Volume	78
DOIs	https://doi.org/10.1016/j.msec.2017.04.021
Publication status	Published - 2017 Sep 1

Keywords

Embryonic stem cell
Feeder-free culture
Pluripotent stem cell
Surface modification
UV/ozone treatment
X-ray photoelectron spectroscopy

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

Access to Document

10.1016/j.msec.2017.04.021

Fingerprint Dive into the research topics of 'Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates'. Together they form a unique fingerprint.

Cite this

@article{9ad81138b30e4d5fb70bc037197bcd09,

title = "Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates",

abstract = "Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.",

keywords = "Embryonic stem cell, Feeder-free culture, Pluripotent stem cell, Surface modification, UV/ozone treatment, X-ray photoelectron spectroscopy",

author = "Kohei Kasai and Yuka Kimura and Shogo Miyata",

year = "2017",

month = sep,

day = "1",

doi = "10.1016/j.msec.2017.04.021",

language = "English",

volume = "78",

pages = "354--361",

journal = "Materials Science and Engineering C",

issn = "0928-4931",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates

AU - Kasai, Kohei

AU - Kimura, Yuka

AU - Miyata, Shogo

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.

AB - Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.

KW - Embryonic stem cell

KW - Feeder-free culture

KW - Pluripotent stem cell

KW - Surface modification

KW - UV/ozone treatment

KW - X-ray photoelectron spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85018510108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018510108&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2017.04.021

DO - 10.1016/j.msec.2017.04.021

M3 - Article

C2 - 28575995

AN - SCOPUS:85018510108

VL - 78

SP - 354

EP - 361

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

ER -