TY - JOUR
T1 - Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates
AU - Kasai, Kohei
AU - Kimura, Yuka
AU - Miyata, Shogo
N1 - Funding Information:
This research is partially supported by Japan Agency for Medical Research and Development (AMED).
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.
AB - Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.
KW - Embryonic stem cell
KW - Feeder-free culture
KW - Pluripotent stem cell
KW - Surface modification
KW - UV/ozone treatment
KW - X-ray photoelectron spectroscopy
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U2 - 10.1016/j.msec.2017.04.021
DO - 10.1016/j.msec.2017.04.021
M3 - Article
C2 - 28575995
AN - SCOPUS:85018510108
VL - 78
SP - 354
EP - 361
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
ER -