Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates

Kohei Kasai, Yuka Kimura, Shogo Miyata

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.

Original languageEnglish
Pages (from-to)354-361
Number of pages8
JournalMaterials Science and Engineering C
Volume78
DOIs
Publication statusPublished - 2017 Sep 1

Keywords

  • Embryonic stem cell
  • Feeder-free culture
  • Pluripotent stem cell
  • Surface modification
  • UV/ozone treatment
  • X-ray photoelectron spectroscopy

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Fingerprint Dive into the research topics of 'Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates'. Together they form a unique fingerprint.

  • Cite this