TY - JOUR
T1 - Improvement of hydrogelation abilities and handling of photocurable gelatin-based crosslinking materials
AU - Fukaya, Chie
AU - Nakayama, Yasuhide
AU - Murayama, Yoshinobu
AU - Omata, Sadao
AU - Ishikawa, Ayaka
AU - Hosaka, Yasuo
AU - Nakagawa, Taneaki
PY - 2009/10/1
Y1 - 2009/10/1
N2 - Three types of eosin-derivatized gelatins (eosin-gelatins) with different molecular weights (Mw) of ca. 15 kDa (low-molecular-weight eosin-gelatin, LEG), ca. 30 kDa (medium-molecular-weight eosin-gelatin, MEG), and ca. 95 kDa (high-molecular-weight eosin-gelatin, HEG) were prepared. All the eosin-gelatins except for HEG dissolved completely in water at 37°C within several hours even at high concentrations of 35 or 40 wt % along with polyamine (poly(N,N-dimethylaminopropylacrylamide)) to produce photo-crosslinkable materials. The materials had appropriate viscosity for in situ molding at 37°C and could be handled as a liquid at low temperatures of up to 25°C. Upon photoirradiation for several tens of seconds, the materials were converted almost completely to hydrogels in the desired form with a microporous network structure by the radical coupling reaction. The mechanical strength of the produced hydrogels could be controlled by selecting a particular molecular weight or concentration of eosin-gelatins. The hydrogels obtained from LEG (40 wt %) or MEG (35 wt %) had elasticity similar to that of goat periodontal tissue. The handling of the photo-crosslinkable materials at room temperature and their photogelation ability were drastically improved by reducing the M w of eosin-gelatin. The potential usefulness of the photo-crosslinkable materials to periodontal regeneration has been discussed.
AB - Three types of eosin-derivatized gelatins (eosin-gelatins) with different molecular weights (Mw) of ca. 15 kDa (low-molecular-weight eosin-gelatin, LEG), ca. 30 kDa (medium-molecular-weight eosin-gelatin, MEG), and ca. 95 kDa (high-molecular-weight eosin-gelatin, HEG) were prepared. All the eosin-gelatins except for HEG dissolved completely in water at 37°C within several hours even at high concentrations of 35 or 40 wt % along with polyamine (poly(N,N-dimethylaminopropylacrylamide)) to produce photo-crosslinkable materials. The materials had appropriate viscosity for in situ molding at 37°C and could be handled as a liquid at low temperatures of up to 25°C. Upon photoirradiation for several tens of seconds, the materials were converted almost completely to hydrogels in the desired form with a microporous network structure by the radical coupling reaction. The mechanical strength of the produced hydrogels could be controlled by selecting a particular molecular weight or concentration of eosin-gelatins. The hydrogels obtained from LEG (40 wt %) or MEG (35 wt %) had elasticity similar to that of goat periodontal tissue. The handling of the photo-crosslinkable materials at room temperature and their photogelation ability were drastically improved by reducing the M w of eosin-gelatin. The potential usefulness of the photo-crosslinkable materials to periodontal regeneration has been discussed.
KW - Eosin
KW - Gelatin
KW - Hydrogel
KW - Periodontal regeneration
KW - Photo-crosslinking
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U2 - 10.1002/jbm.b.31406
DO - 10.1002/jbm.b.31406
M3 - Article
C2 - 19441120
AN - SCOPUS:70049083967
VL - 91
SP - 329
EP - 336
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
SN - 1552-4973
IS - 1
ER -