Improvement of hydrogelation abilities and handling of photocurable gelatin-based crosslinking materials

Chie Fukaya, Yasuhide Nakayama, Yoshinobu Murayama, Sadao Omata, Ayaka Ishikawa, Yasuo Hosaka, Taneaki Nakagawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Three types of eosin-derivatized gelatins (eosin-gelatins) with different molecular weights (Mw) of ca. 15 kDa (low-molecular-weight eosin-gelatin, LEG), ca. 30 kDa (medium-molecular-weight eosin-gelatin, MEG), and ca. 95 kDa (high-molecular-weight eosin-gelatin, HEG) were prepared. All the eosin-gelatins except for HEG dissolved completely in water at 37°C within several hours even at high concentrations of 35 or 40 wt % along with polyamine (poly(N,N-dimethylaminopropylacrylamide)) to produce photo-crosslinkable materials. The materials had appropriate viscosity for in situ molding at 37°C and could be handled as a liquid at low temperatures of up to 25°C. Upon photoirradiation for several tens of seconds, the materials were converted almost completely to hydrogels in the desired form with a microporous network structure by the radical coupling reaction. The mechanical strength of the produced hydrogels could be controlled by selecting a particular molecular weight or concentration of eosin-gelatins. The hydrogels obtained from LEG (40 wt %) or MEG (35 wt %) had elasticity similar to that of goat periodontal tissue. The handling of the photo-crosslinkable materials at room temperature and their photogelation ability were drastically improved by reducing the M w of eosin-gelatin. The potential usefulness of the photo-crosslinkable materials to periodontal regeneration has been discussed.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume91
Issue number1
DOIs
Publication statusPublished - 2009 Oct 1

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Keywords

  • Eosin
  • Gelatin
  • Hydrogel
  • Periodontal regeneration
  • Photo-crosslinking

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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