In early development of the rat mRNA for the major myelin protein P0 is expressed in nonsensory areas of the embryonic inner ear, notochord, enteric nervous system, and olfactory ensheathing cells

Maria Kolatsi-Joannou, Xiao Z. Li, Toshio Suda, Hai T. Yuan, Adrian S. Woolf

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The myelin protein P0 has a major structural role in Schwann cell myelin, and the expression of P0 protein and mRNA in the Schwann cell lineage has been extensively documented. We show here, using in situ hybridization, that the P0 gene is also activated in a number of other tissues during embryonic development. P0 mRNA is first detectable in 10-day-old embryos (E10) and is at this time seen only in cells in the cephalic neural crest and in the otic placode/pit. P0 expression continues in the otic vesicle and at E12 P0 expression in this structure largely overlaps with expression of another myelin gene, proteolipid protein. In the developing ear at E14, P0 expression is complementary to expression of serrate and c-ret mRNAs, which later are expressed in sensory areas of the inner ear, while expression of bone morphogenetic protein (BMP)-4 and P0, though largely complementary, shows small areas of overlap. P0 mRNA and protein are detectable in the notochord from E10 to at least E13. In addition to P0 expression in a subpopulation of trunk crest cells at E11/E12 and in Schwann cell precursors thereafter, P0 mRNA is also present transiently in a subpopulation of cells migrating in the enteric neural crest pathway, but is down-regulated in these cells at E14 and thereafter. P0 is also detected in the placode-derived olfactory ensheathing cells from E13 and is maintained in the adult. No signal is seen in cells in the melanocyte migration pathway or in TUJ1 positive neuronal cells in tissue sections. The activation of the P0 gene in specific tissues outside the nervous system was unexpected. It remains to be determined whether this is functionally significant, or whether it is an evolutionary relic, perhaps reflecting ancestral use of P0 as an adhesion molecule.

Original languageEnglish
Pages (from-to)40-51
Number of pages12
JournalDevelopmental Dynamics
Volume222
Issue number1
DOIs
Publication statusPublished - 2001

Fingerprint

Myelin P0 Protein
Enteric Nervous System
Notochord
Inner Ear
Messenger RNA
Schwann Cells
Neural Crest
Ear
Myelin Proteolipid Protein
Bone Morphogenetic Protein 4
Neural Pathways
Melanocytes
Cell Lineage
Nervous System
Transcriptional Activation
Embryonic Development
In Situ Hybridization
Embryonic Structures
Head

Keywords

  • BMP-4
  • c-ret
  • Glia
  • Neural crest
  • Otic pit
  • Otic vesicle
  • Schwann cells
  • Serrate

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

In early development of the rat mRNA for the major myelin protein P0 is expressed in nonsensory areas of the embryonic inner ear, notochord, enteric nervous system, and olfactory ensheathing cells. / Kolatsi-Joannou, Maria; Li, Xiao Z.; Suda, Toshio; Yuan, Hai T.; Woolf, Adrian S.

In: Developmental Dynamics, Vol. 222, No. 1, 2001, p. 40-51.

Research output: Contribution to journalArticle

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abstract = "The myelin protein P0 has a major structural role in Schwann cell myelin, and the expression of P0 protein and mRNA in the Schwann cell lineage has been extensively documented. We show here, using in situ hybridization, that the P0 gene is also activated in a number of other tissues during embryonic development. P0 mRNA is first detectable in 10-day-old embryos (E10) and is at this time seen only in cells in the cephalic neural crest and in the otic placode/pit. P0 expression continues in the otic vesicle and at E12 P0 expression in this structure largely overlaps with expression of another myelin gene, proteolipid protein. In the developing ear at E14, P0 expression is complementary to expression of serrate and c-ret mRNAs, which later are expressed in sensory areas of the inner ear, while expression of bone morphogenetic protein (BMP)-4 and P0, though largely complementary, shows small areas of overlap. P0 mRNA and protein are detectable in the notochord from E10 to at least E13. In addition to P0 expression in a subpopulation of trunk crest cells at E11/E12 and in Schwann cell precursors thereafter, P0 mRNA is also present transiently in a subpopulation of cells migrating in the enteric neural crest pathway, but is down-regulated in these cells at E14 and thereafter. P0 is also detected in the placode-derived olfactory ensheathing cells from E13 and is maintained in the adult. No signal is seen in cells in the melanocyte migration pathway or in TUJ1 positive neuronal cells in tissue sections. The activation of the P0 gene in specific tissues outside the nervous system was unexpected. It remains to be determined whether this is functionally significant, or whether it is an evolutionary relic, perhaps reflecting ancestral use of P0 as an adhesion molecule.",
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