In planta functions of cytochrome P450 monooxygenase genes in the phytocassane biosynthetic gene cluster on rice chromosome 2

Zhongfeng Ye, Kohei Yamazaki, Hiromi Minoda, Koji Miyamoto, Sho Miyazaki, Hiroshi Kawaide, Arata Yajima, Hideaki Nojiri, Hisakazu Yamane, Kazunori Okada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In response to environmental stressors such as blast fungal infections, rice produces phytoalexins, an antimicrobial diterpenoid compound. Together with momilactones, phytocassanes are among the major diterpenoid phytoalexins. The biosynthetic genes of diterpenoid phytoalexin are organized on the chromosome in functional gene clusters, comprising diterpene cyclase, dehydrogenase, and cytochrome P450 monooxygenase genes. Their functions have been studied extensively using in vitro enzyme assay systems. Specifically, P450 genes (CYP71Z6, Z7; CYP76M5, M6, M7, M8) on rice chromosome 2 have multifunctional activities associated with ent-copalyl diphosphate-related diterpene hydrocarbons, but the in planta contribution of these genes to diterpenoid phytoalexin production remains unknown. Here, we characterized cyp71z7 T-DNA mutant and CYP76M7/M8 RNAi lines to find that potential phytoalexin intermediates accumulated in these P450-suppressed rice plants. The results suggested that in planta, CYP71Z7 is responsible for C2-hydroxylation of phytocassanes and that CYP76M7/M8 is involved in C11α-hydroxylation of 3-hydroxy-cassadiene. Based on these results, we proposed potential routes of phytocassane biosynthesis in planta.

Original languageEnglish
Pages (from-to)1021-1030
Number of pages10
JournalBioscience, Biotechnology and Biochemistry
Volume82
Issue number6
DOIs
Publication statusPublished - 2018 Jan 1
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 2
Diterpenes
Chromosomes
Multigene Family
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Genes
Hydroxylation
Mycoses
Biosynthesis
Enzyme Assays
Hydrocarbons
RNA Interference
Oryza
Assays
Oxidoreductases
phytoalexins
Enzymes

Keywords

  • Cytochrome P450 monooxygenase
  • Diterpene phytoalexins
  • Gene cluster
  • Rice

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

In planta functions of cytochrome P450 monooxygenase genes in the phytocassane biosynthetic gene cluster on rice chromosome 2. / Ye, Zhongfeng; Yamazaki, Kohei; Minoda, Hiromi; Miyamoto, Koji; Miyazaki, Sho; Kawaide, Hiroshi; Yajima, Arata; Nojiri, Hideaki; Yamane, Hisakazu; Okada, Kazunori.

In: Bioscience, Biotechnology and Biochemistry, Vol. 82, No. 6, 01.01.2018, p. 1021-1030.

Research output: Contribution to journalArticle

Ye, Z, Yamazaki, K, Minoda, H, Miyamoto, K, Miyazaki, S, Kawaide, H, Yajima, A, Nojiri, H, Yamane, H & Okada, K 2018, 'In planta functions of cytochrome P450 monooxygenase genes in the phytocassane biosynthetic gene cluster on rice chromosome 2', Bioscience, Biotechnology and Biochemistry, vol. 82, no. 6, pp. 1021-1030. https://doi.org/10.1080/09168451.2017.1398067
Ye, Zhongfeng ; Yamazaki, Kohei ; Minoda, Hiromi ; Miyamoto, Koji ; Miyazaki, Sho ; Kawaide, Hiroshi ; Yajima, Arata ; Nojiri, Hideaki ; Yamane, Hisakazu ; Okada, Kazunori. / In planta functions of cytochrome P450 monooxygenase genes in the phytocassane biosynthetic gene cluster on rice chromosome 2. In: Bioscience, Biotechnology and Biochemistry. 2018 ; Vol. 82, No. 6. pp. 1021-1030.
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