In situ gelation properties of a collagen-genipin sol with a potential for the treatment of gastrointestinal ulcers

Takefumi Narita, Shunji Yunoki, Yoshimi Ohyabu, Naohisa Yahagi, Toshio Uraoka

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We investigated the potential of collagen-genipin sols as biomaterials for treating artificial ulcers following endoscopic submucosal dissection. Collagen sol viscosity increased with condensation, allowing retention on tilted ulcers before gelation and resulting in collagen gel deposition on whole ulcers. The 1.44% collagen sols containing genipin as a crosslinker retained sol fluidity at 23°C for >20 min, facilitating endoscopic use. Collagen sols formed gel depositions on artificial ulcers in response to body temperature, and high temperature responsiveness of gelation because of increased neutral phosphate buffer concentration allowed for thick gel deposition on tilted ulcers. Finally, histological observations showed infiltration of gels into submucosal layers. Taken together, the present data show that genipin-induced crosslinking significantly improves the mechanical properties of collagen gels even at low genipin concentrations of 0.2-1 mM, warranting the use of in situ gelling collagen-genipin sols for endoscopic treatments of gastrointestinal ulcers.

Original languageEnglish
Pages (from-to)429-439
Number of pages11
JournalMedical Devices: Evidence and Research
Volume9
DOIs
Publication statusPublished - 2016 Dec 15

Keywords

  • Collagen
  • Fibril formation
  • Genipin
  • In-situ gel
  • Ulcer dressing

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

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