Abstract
In vitro anti-DNA antibody synthesis by peripheral blood lymphocytes from systemic lupus erythematosus (SLE) patients after native DNA (nDNA) stimulation was studied by the Farr assay after digestion with deoxyribonuclease I. Active SLE lymphocytes synthetized IgG and anti-DNA antibody with nDNA stimulation but inactive SLE and normal lymphocytes did not. Binding of 14C-labeled Ig and [3H]DNA showed that DNA-binding Ig was actively secreted by SLE lymphocytes. SLE antilymphocyte antibody (ALA)-sensitive T cells (SLE+ T cells) and unreactive T cells (SLE- T cells) were separated by anti-human Ig antibody-coated dishes to determine their effect on antibody synthesis. Inactive SLE B cells synthetized both IgG and anti-DNA antibody after nDNA stimulation with autologous SLE- T cells but stimulation with SLE+ T cells did not result in synthesis of either. Normal B cells did not synthetize IgG even with autologous SLE- T cells. These results indicate that active and inactive SLE patients have autoreactive lymphocytes against nDNA which synthetize anti-DNA antibody and are regulated by ALA-sensitive T cells (possibly deficient in active SLE).
| Original language | English |
|---|---|
| Pages (from-to) | 237-246 |
| Number of pages | 10 |
| Journal | Clinical Immunology and Immunopathology |
| Volume | 21 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1981 |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Pathology and Forensic Medicine
Cite this
In vitro anti-DNA antibody synthesis by peripheral blood lymphocytes in patients with systemic lupus erythematosus. / Kiyotaki, Masaya; Toguchi, Takashi; Abe, Tohru; Takeuchi, Tsutomu; Morimoto, Chikao; Homma, Mitsuo.
In: Clinical Immunology and Immunopathology, Vol. 21, No. 2, 1981, p. 237-246.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro anti-DNA antibody synthesis by peripheral blood lymphocytes in patients with systemic lupus erythematosus
AU - Kiyotaki, Masaya
AU - Toguchi, Takashi
AU - Abe, Tohru
AU - Takeuchi, Tsutomu
AU - Morimoto, Chikao
AU - Homma, Mitsuo
PY - 1981
Y1 - 1981
N2 - In vitro anti-DNA antibody synthesis by peripheral blood lymphocytes from systemic lupus erythematosus (SLE) patients after native DNA (nDNA) stimulation was studied by the Farr assay after digestion with deoxyribonuclease I. Active SLE lymphocytes synthetized IgG and anti-DNA antibody with nDNA stimulation but inactive SLE and normal lymphocytes did not. Binding of 14C-labeled Ig and [3H]DNA showed that DNA-binding Ig was actively secreted by SLE lymphocytes. SLE antilymphocyte antibody (ALA)-sensitive T cells (SLE+ T cells) and unreactive T cells (SLE- T cells) were separated by anti-human Ig antibody-coated dishes to determine their effect on antibody synthesis. Inactive SLE B cells synthetized both IgG and anti-DNA antibody after nDNA stimulation with autologous SLE- T cells but stimulation with SLE+ T cells did not result in synthesis of either. Normal B cells did not synthetize IgG even with autologous SLE- T cells. These results indicate that active and inactive SLE patients have autoreactive lymphocytes against nDNA which synthetize anti-DNA antibody and are regulated by ALA-sensitive T cells (possibly deficient in active SLE).
AB - In vitro anti-DNA antibody synthesis by peripheral blood lymphocytes from systemic lupus erythematosus (SLE) patients after native DNA (nDNA) stimulation was studied by the Farr assay after digestion with deoxyribonuclease I. Active SLE lymphocytes synthetized IgG and anti-DNA antibody with nDNA stimulation but inactive SLE and normal lymphocytes did not. Binding of 14C-labeled Ig and [3H]DNA showed that DNA-binding Ig was actively secreted by SLE lymphocytes. SLE antilymphocyte antibody (ALA)-sensitive T cells (SLE+ T cells) and unreactive T cells (SLE- T cells) were separated by anti-human Ig antibody-coated dishes to determine their effect on antibody synthesis. Inactive SLE B cells synthetized both IgG and anti-DNA antibody after nDNA stimulation with autologous SLE- T cells but stimulation with SLE+ T cells did not result in synthesis of either. Normal B cells did not synthetize IgG even with autologous SLE- T cells. These results indicate that active and inactive SLE patients have autoreactive lymphocytes against nDNA which synthetize anti-DNA antibody and are regulated by ALA-sensitive T cells (possibly deficient in active SLE).
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UR - http://www.scopus.com/inward/citedby.url?scp=0019456799&partnerID=8YFLogxK
U2 - 10.1016/0090-1229(81)90212-9
DO - 10.1016/0090-1229(81)90212-9
M3 - Article
C2 - 6975193
AN - SCOPUS:0019456799
VL - 21
SP - 237
EP - 246
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 2
ER -