In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells

Taisuke Kondo, Yuki Imura, Makoto Ando, Shunsuke Chikuma, Akihiko Yoshimura

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (TSCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically TSCM cells (iTSCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iTSCM cells, including reagents, culture setting, and procedure.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages41-51
Number of pages11
DOIs
Publication statusPublished - 2019 Jan 1

Publication series

NameMethods in Molecular Biology
Volume2048
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Adoptive T cell therapy
  • Cancer immunotherapy
  • Coculture with feeder cells
  • Notch signaling
  • Stem cell memory T cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Kondo, T., Imura, Y., Ando, M., Chikuma, S., & Yoshimura, A. (2019). In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells. In Methods in Molecular Biology (pp. 41-51). (Methods in Molecular Biology; Vol. 2048). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9728-2_4