In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells

Taisuke Kondo, Yuki Imura, Makoto Ando, Shunsuke Chikuma, Akihiko Yoshimura

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (TSCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically TSCM cells (iTSCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iTSCM cells, including reagents, culture setting, and procedure.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages41-51
Number of pages11
DOIs
Publication statusPublished - 2019 Jan 1

Publication series

NameMethods in Molecular Biology
Volume2048
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Stem Cells
T-Lymphocytes
Neoplasm Antigens
Cell- and Tissue-Based Therapy
Coculture Techniques
Immunotherapy
Neoplasms
In Vitro Techniques
Ligands
Phenotype

Keywords

  • Adoptive T cell therapy
  • Cancer immunotherapy
  • Coculture with feeder cells
  • Notch signaling
  • Stem cell memory T cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kondo, T., Imura, Y., Ando, M., Chikuma, S., & Yoshimura, A. (2019). In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells. In Methods in Molecular Biology (pp. 41-51). (Methods in Molecular Biology; Vol. 2048). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9728-2_4

In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells. / Kondo, Taisuke; Imura, Yuki; Ando, Makoto; Chikuma, Shunsuke; Yoshimura, Akihiko.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 41-51 (Methods in Molecular Biology; Vol. 2048).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kondo, T, Imura, Y, Ando, M, Chikuma, S & Yoshimura, A 2019, In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2048, Humana Press Inc., pp. 41-51. https://doi.org/10.1007/978-1-4939-9728-2_4
Kondo T, Imura Y, Ando M, Chikuma S, Yoshimura A. In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 41-51. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9728-2_4
Kondo, Taisuke ; Imura, Yuki ; Ando, Makoto ; Chikuma, Shunsuke ; Yoshimura, Akihiko. / In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 41-51 (Methods in Molecular Biology).
@inbook{424498c5208c47c98303342489a175c0,
title = "In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells",
abstract = "Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (TSCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically TSCM cells (iTSCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iTSCM cells, including reagents, culture setting, and procedure.",
keywords = "Adoptive T cell therapy, Cancer immunotherapy, Coculture with feeder cells, Notch signaling, Stem cell memory T cells",
author = "Taisuke Kondo and Yuki Imura and Makoto Ando and Shunsuke Chikuma and Akihiko Yoshimura",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-9728-2_4",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "41--51",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells

AU - Kondo, Taisuke

AU - Imura, Yuki

AU - Ando, Makoto

AU - Chikuma, Shunsuke

AU - Yoshimura, Akihiko

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (TSCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically TSCM cells (iTSCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iTSCM cells, including reagents, culture setting, and procedure.

AB - Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (TSCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically TSCM cells (iTSCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iTSCM cells, including reagents, culture setting, and procedure.

KW - Adoptive T cell therapy

KW - Cancer immunotherapy

KW - Coculture with feeder cells

KW - Notch signaling

KW - Stem cell memory T cells

UR - http://www.scopus.com/inward/record.url?scp=85071280427&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071280427&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9728-2_4

DO - 10.1007/978-1-4939-9728-2_4

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 41

EP - 51

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -