In vitro formation of organized structure between keratinocytes and dorsal-root-ganglion cells

Moe Tsutsumi, Masashi Nakatani, Junichi Kumamoto, Sumiko Denda, Mitsuhiro Denda

Research output: Contribution to journalLetter

4 Citations (Scopus)

Abstract

We recently found that the morphology in a co-culture system of keratinocytes and dorsal-root ganglion-derived cells depended on the timing of seeding of the two cell types.In skin, epidermis is formed first, followed by construction of peripheral nerve structure. Therefore, we hypothesized that formation of peripheral nerve structure in the epidermis might be driven by interaction between keratinocytes and nerve cells. In the present study, we tested this idea by incubating keratinocytes and dorsalroot ganglion cells in a spatially separated manner and observing the morphological changes in the co-culture system. Extension of nerve fibre-like structures from the ganglion cells was observed, and within 3 days after seeding, many nerve fibre-like extensions penetrated into the keratinocyte cluster, subsequently forming a network that appeared to resemble the cutaneous peripheral nervous system. Our present model may be useful for studying the formation of peripheral nerve structure in the skin.

Original languageEnglish
Pages (from-to)886-888
Number of pages3
JournalExperimental Dermatology
Volume21
Issue number11
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Spinal Ganglia
Keratinocytes
Peripheral Nerves
Coculture Techniques
Nerve Fibers
Epidermis
Ganglia
Skin
Fibers
Peripheral Nervous System
Neurology
Neurons
In Vitro Techniques

Keywords

  • Co
  • Culture
  • Epidermis
  • Nerve fiber
  • Peripheral nerve structure
  • Schwann cell

ASJC Scopus subject areas

  • Dermatology
  • Molecular Biology
  • Biochemistry

Cite this

In vitro formation of organized structure between keratinocytes and dorsal-root-ganglion cells. / Tsutsumi, Moe; Nakatani, Masashi; Kumamoto, Junichi; Denda, Sumiko; Denda, Mitsuhiro.

In: Experimental Dermatology, Vol. 21, No. 11, 2012, p. 886-888.

Research output: Contribution to journalLetter

Tsutsumi, Moe ; Nakatani, Masashi ; Kumamoto, Junichi ; Denda, Sumiko ; Denda, Mitsuhiro. / In vitro formation of organized structure between keratinocytes and dorsal-root-ganglion cells. In: Experimental Dermatology. 2012 ; Vol. 21, No. 11. pp. 886-888.
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