In vitro free radical scavenging activity of platinum nanoparticles

Aki Watanabe, Masashi Kajita, Juewon Kim, Atsuhiro Kanayama, Kyoko Takahashi, Tadahiko Mashino, Yusei Miyamoto

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

A polyacrylic acid (PAA)-protected platinum nanoparticle species (PAA-Pt) was prepared by alcohol reduction of hexachloroplatinate. The PAA-Pt nanoparticles were well dispersed and homogeneous in size with an average diameter of 2.0 0.4nm (n = 200). We used electron spin resonance to quantify the residual peroxyl radical () generated from 2,2-azobis (2-aminopropane) dihydrochloride (AAPH) by thermal decomposition in the presence of O2 and a spectrophotometric method to quantify the residual 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical. PAA-Pt scavenged these two radicals in a dose-dependent manner. Platinum was the functional component. PAA-Pt reduced the rate of oxygen consumption required for linoleic acid peroxidation initiated by generated from AAPH, indicating inhibition of the propagation of linolate peroxidation. A thiobarbituric acid test also revealed dose-dependent inhibition of the linolate peroxidation by PAA-Pt. Fifty micromolar platinum, as PAA-Pt, completely quenched 250 νM DPPH radical for 5min. Even when twice diluted in half, the PAA-Pt still quenched 100% of the 250νM DPPH radical. The scavenging activity of PAA-Pt is durable. These observations suggest that PAA-Pt is an efficient scavenger of free radicals.

Original languageEnglish
Article number455105
JournalNanotechnology
Volume20
Issue number45
DOIs
Publication statusPublished - 2009

Fingerprint

carbopol 940
Scavenging
Platinum
Free radicals
Free Radicals
Nanoparticles
Acids
Free Radical Scavengers
Linoleic acid

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

In vitro free radical scavenging activity of platinum nanoparticles. / Watanabe, Aki; Kajita, Masashi; Kim, Juewon; Kanayama, Atsuhiro; Takahashi, Kyoko; Mashino, Tadahiko; Miyamoto, Yusei.

In: Nanotechnology, Vol. 20, No. 45, 455105, 2009.

Research output: Contribution to journalArticle

Watanabe, Aki ; Kajita, Masashi ; Kim, Juewon ; Kanayama, Atsuhiro ; Takahashi, Kyoko ; Mashino, Tadahiko ; Miyamoto, Yusei. / In vitro free radical scavenging activity of platinum nanoparticles. In: Nanotechnology. 2009 ; Vol. 20, No. 45.
@article{0b9e4830784f4e7c9cff3139b00df7f3,
title = "In vitro free radical scavenging activity of platinum nanoparticles",
abstract = "A polyacrylic acid (PAA)-protected platinum nanoparticle species (PAA-Pt) was prepared by alcohol reduction of hexachloroplatinate. The PAA-Pt nanoparticles were well dispersed and homogeneous in size with an average diameter of 2.0 0.4nm (n = 200). We used electron spin resonance to quantify the residual peroxyl radical () generated from 2,2-azobis (2-aminopropane) dihydrochloride (AAPH) by thermal decomposition in the presence of O2 and a spectrophotometric method to quantify the residual 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical. PAA-Pt scavenged these two radicals in a dose-dependent manner. Platinum was the functional component. PAA-Pt reduced the rate of oxygen consumption required for linoleic acid peroxidation initiated by generated from AAPH, indicating inhibition of the propagation of linolate peroxidation. A thiobarbituric acid test also revealed dose-dependent inhibition of the linolate peroxidation by PAA-Pt. Fifty micromolar platinum, as PAA-Pt, completely quenched 250 νM DPPH radical for 5min. Even when twice diluted in half, the PAA-Pt still quenched 100{\%} of the 250νM DPPH radical. The scavenging activity of PAA-Pt is durable. These observations suggest that PAA-Pt is an efficient scavenger of free radicals.",
author = "Aki Watanabe and Masashi Kajita and Juewon Kim and Atsuhiro Kanayama and Kyoko Takahashi and Tadahiko Mashino and Yusei Miyamoto",
year = "2009",
doi = "10.1088/0957-4484/20/45/455105",
language = "English",
volume = "20",
journal = "Nanotechnology",
issn = "0957-4484",
publisher = "IOP Publishing Ltd.",
number = "45",

}

TY - JOUR

T1 - In vitro free radical scavenging activity of platinum nanoparticles

AU - Watanabe, Aki

AU - Kajita, Masashi

AU - Kim, Juewon

AU - Kanayama, Atsuhiro

AU - Takahashi, Kyoko

AU - Mashino, Tadahiko

AU - Miyamoto, Yusei

PY - 2009

Y1 - 2009

N2 - A polyacrylic acid (PAA)-protected platinum nanoparticle species (PAA-Pt) was prepared by alcohol reduction of hexachloroplatinate. The PAA-Pt nanoparticles were well dispersed and homogeneous in size with an average diameter of 2.0 0.4nm (n = 200). We used electron spin resonance to quantify the residual peroxyl radical () generated from 2,2-azobis (2-aminopropane) dihydrochloride (AAPH) by thermal decomposition in the presence of O2 and a spectrophotometric method to quantify the residual 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical. PAA-Pt scavenged these two radicals in a dose-dependent manner. Platinum was the functional component. PAA-Pt reduced the rate of oxygen consumption required for linoleic acid peroxidation initiated by generated from AAPH, indicating inhibition of the propagation of linolate peroxidation. A thiobarbituric acid test also revealed dose-dependent inhibition of the linolate peroxidation by PAA-Pt. Fifty micromolar platinum, as PAA-Pt, completely quenched 250 νM DPPH radical for 5min. Even when twice diluted in half, the PAA-Pt still quenched 100% of the 250νM DPPH radical. The scavenging activity of PAA-Pt is durable. These observations suggest that PAA-Pt is an efficient scavenger of free radicals.

AB - A polyacrylic acid (PAA)-protected platinum nanoparticle species (PAA-Pt) was prepared by alcohol reduction of hexachloroplatinate. The PAA-Pt nanoparticles were well dispersed and homogeneous in size with an average diameter of 2.0 0.4nm (n = 200). We used electron spin resonance to quantify the residual peroxyl radical () generated from 2,2-azobis (2-aminopropane) dihydrochloride (AAPH) by thermal decomposition in the presence of O2 and a spectrophotometric method to quantify the residual 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical. PAA-Pt scavenged these two radicals in a dose-dependent manner. Platinum was the functional component. PAA-Pt reduced the rate of oxygen consumption required for linoleic acid peroxidation initiated by generated from AAPH, indicating inhibition of the propagation of linolate peroxidation. A thiobarbituric acid test also revealed dose-dependent inhibition of the linolate peroxidation by PAA-Pt. Fifty micromolar platinum, as PAA-Pt, completely quenched 250 νM DPPH radical for 5min. Even when twice diluted in half, the PAA-Pt still quenched 100% of the 250νM DPPH radical. The scavenging activity of PAA-Pt is durable. These observations suggest that PAA-Pt is an efficient scavenger of free radicals.

UR - http://www.scopus.com/inward/record.url?scp=70350645171&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350645171&partnerID=8YFLogxK

U2 - 10.1088/0957-4484/20/45/455105

DO - 10.1088/0957-4484/20/45/455105

M3 - Article

VL - 20

JO - Nanotechnology

JF - Nanotechnology

SN - 0957-4484

IS - 45

M1 - 455105

ER -