In vitro gene delivery mediated by chitosan. Effect of pH, serum, and molecular mass of chitosan on the transfection efficiency

Toshinori Sato, Tsuyoshi Ishii, Yoshio Okahata

Research output: Contribution to journalArticle

400 Citations (Scopus)

Abstract

Aminopolysaccharides such as chitosan and polygalactosamine (pGalN) were used to transfer luciferase plasmid into tumor cells. Chitosan largely enhanced the transfection efficiency of luciferase plasmid (pGL3), while pGalN did not at all. Transfection efficiencies of the pGL3/chitosan complexes were dependent on pH of culture medium, stoicheometry of pGL3:chitosan, serum, and molecular mass of chitosan. Transfection efficiency at pH 6.9 was higher than that at pH7.6. Optimum charge ratio of the pGL3:chitosan was 1:5. Chitosan polymer of 15 and 52 kDa largely promoted luciferase activities. Transfection efficiency mediated by chitosan of > 100 kDa was less than that by chitosan of 15 and 52 kDa. Heptamer (1.3 kDa) did not show any gene expression. Cationic liposome (lipofectin)-associated gene expression was inhibited by serum, while chitosan showed resistance to serum.

Original languageEnglish
Pages (from-to)2075-2080
Number of pages6
JournalBiomaterials
Volume22
Issue number15
DOIs
Publication statusPublished - 2001 Aug 1

Fingerprint

Chitosan
Molecular mass
Transfection
Genes
Serum
Luciferases
Gene expression
Plasmids
In Vitro Techniques
Gene Expression
Liposomes
Culture Media
Tumors
Polymers
Cells

Keywords

  • Chitosan
  • Gene transfer
  • Lipofectin
  • Luciferase plasmid
  • Polygalactosamine
  • Transfection

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

In vitro gene delivery mediated by chitosan. Effect of pH, serum, and molecular mass of chitosan on the transfection efficiency. / Sato, Toshinori; Ishii, Tsuyoshi; Okahata, Yoshio.

In: Biomaterials, Vol. 22, No. 15, 01.08.2001, p. 2075-2080.

Research output: Contribution to journalArticle

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