In vitro gene transfer by the application of laser-induced stress wave: Effect of cellular heating

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Kuniaki Nakanishi, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, Minoru Obara

    Research output: Contribution to journalConference articlepeer-review

    Abstract

    We previously demonstrated in vitro that the simultaneous application of cellular heating and a laser-induced stress wave (LISW) enhanced the uptake of porfimer sodium (Photofrin) by cells. In this study, we attempted to apply this technique to gene transfer to cultured cells. LISW and/or a transient mild (∼43°C) heating been applied to deliver plasmid coding for green fluorescent protein (EGFP) to NIH-3T3 cells. It was found that simultaneous application of an LISW and the heating significantly increased the transfection efficiency by a factor of 2.5 when compared with that for the cells treated with an LISW alone.

    Original languageEnglish
    Pages (from-to)136-139
    Number of pages4
    JournalProceedings of SPIE - The International Society for Optical Engineering
    Volume5322
    DOIs
    Publication statusPublished - 2004 Oct 27
    EventProgress in Biomedical Optics and Imaging - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II - San Jose, CA, United States
    Duration: 2004 Jan 272004 Jan 28

    Keywords

    • Gene therapy
    • Heat
    • Laser
    • Membrane fluidity
    • Stress wave

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Condensed Matter Physics
    • Computer Science Applications
    • Applied Mathematics
    • Electrical and Electronic Engineering

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