In vitro gene transfer by the application of laser-induced stress wave: Effect of cellular heating

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Kuniaki Nakanishi, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, Minoru Obara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We previously demonstrated in vitro that the simultaneous application of cellular heating and a laser-induced stress wave (LISW) enhanced the uptake of porfimer sodium (Photofrin) by cells. In this study, we attempted to apply this technique to gene transfer to cultured cells. LISW and/or a transient mild (∼43°C) heating been applied to deliver plasmid coding for green fluorescent protein (EGFP) to NIH-3T3 cells. It was found that simultaneous application of an LISW and the heating significantly increased the transfection efficiency by a factor of 2.5 when compared with that for the cells treated with an LISW alone.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsD.V. Nicolau, J. Enderlein, R.C. Leif, D.L. Farkas
Pages136-139
Number of pages4
Volume5322
DOIs
Publication statusPublished - 2004
EventProgress in Biomedical Optics and Imaging - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II - San Jose, CA, United States
Duration: 2004 Jan 272004 Jan 28

Other

OtherProgress in Biomedical Optics and Imaging - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II
CountryUnited States
CitySan Jose, CA
Period04/1/2704/1/28

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Keywords

  • Gene therapy
  • Heat
  • Laser
  • Membrane fluidity
  • Stress wave

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Terakawa, M., Sato, S., Ogura, M., Nakanishi, K., Masaki, Y., Uenoyama, M., Wakisaka, H., Ashida, H., & Obara, M. (2004). In vitro gene transfer by the application of laser-induced stress wave: Effect of cellular heating. In D. V. Nicolau, J. Enderlein, R. C. Leif, & D. L. Farkas (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 5322, pp. 136-139) https://doi.org/10.1117/12.530815