In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, Minoru Obara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm2, but at higher fluences (>1.7 J/cm2), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsS.L. Jacques, W.P. Roach
Pages225-229
Number of pages5
Volume5695
DOIs
Publication statusPublished - 2005
EventOptical Interactions with Tissue and Cells XVI - San Jose, CA, United States
Duration: 2005 Jan 242005 Jan 26

Other

OtherOptical Interactions with Tissue and Cells XVI
CountryUnited States
CitySan Jose, CA
Period05/1/2405/1/26

Fingerprint

Gene transfer
Wave effects
Laser beam effects
Lasers
Laser pulses
Gene therapy
Controllability
Pulsed lasers
DNA
Genes

Keywords

  • Drug delivery
  • Gene therapy
  • GFP
  • Laser-induced stress wave

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Terakawa, M., Sato, S., Ogura, M., Masaki, Y., Uenoyama, M., Wakisaka, H., ... Obara, M. (2005). In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency. In S. L. Jacques, & W. P. Roach (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 5695, pp. 225-229). [36] https://doi.org/10.1117/12.588852

In vitro gene transfer by the use of laser-induced stress wave : Effect of laser irradiation conditions on transfection efficiency. / Terakawa, Mitsuhiro; Sato, Shunichi; Ogura, Makoto; Masaki, Yoshinori; Uenoyama, Maki; Wakisaka, Hitoshi; Ashida, Hiroshi; Obara, Minoru.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / S.L. Jacques; W.P. Roach. Vol. 5695 2005. p. 225-229 36.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Terakawa, M, Sato, S, Ogura, M, Masaki, Y, Uenoyama, M, Wakisaka, H, Ashida, H & Obara, M 2005, In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency. in SL Jacques & WP Roach (eds), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 5695, 36, pp. 225-229, Optical Interactions with Tissue and Cells XVI, San Jose, CA, United States, 05/1/24. https://doi.org/10.1117/12.588852
Terakawa M, Sato S, Ogura M, Masaki Y, Uenoyama M, Wakisaka H et al. In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency. In Jacques SL, Roach WP, editors, Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 5695. 2005. p. 225-229. 36 https://doi.org/10.1117/12.588852
Terakawa, Mitsuhiro ; Sato, Shunichi ; Ogura, Makoto ; Masaki, Yoshinori ; Uenoyama, Maki ; Wakisaka, Hitoshi ; Ashida, Hiroshi ; Obara, Minoru. / In vitro gene transfer by the use of laser-induced stress wave : Effect of laser irradiation conditions on transfection efficiency. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. editor / S.L. Jacques ; W.P. Roach. Vol. 5695 2005. pp. 225-229
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