In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, Minoru Obara

    Research output: Contribution to journalConference article

    Abstract

    Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm2, but at higher fluences (>1.7 J/cm2), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.

    Original languageEnglish
    Article number36
    Pages (from-to)225-229
    Number of pages5
    JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
    Volume5695
    DOIs
    Publication statusPublished - 2005 Aug 16
    EventOptical Interactions with Tissue and Cells XVI - San Jose, CA, United States
    Duration: 2005 Jan 242005 Jan 26

    Keywords

    • Drug delivery
    • Gene therapy
    • GFP
    • Laser-induced stress wave

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Biomaterials
    • Atomic and Molecular Physics, and Optics
    • Radiology Nuclear Medicine and imaging

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