In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency

Mitsuhiro Terakawa; Shunichi Sato; Makoto Ogura; Yoshinori Masaki; Maki Uenoyama; Hitoshi Wakisaka; Hiroshi Ashida; Minoru Obara

doi:10.1117/12.588852

In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, Minoru Obara

Research output: Contribution to journal › Conference article › peer-review

Abstract

Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm², but at higher fluences (>1.7 J/cm²), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.

Original language	English
Article number	36
Pages (from-to)	225-229
Number of pages	5
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5695
DOIs	https://doi.org/10.1117/12.588852
Publication status	Published - 2005 Aug 16
Event	Optical Interactions with Tissue and Cells XVI - San Jose, CA, United States Duration: 2005 Jan 24 → 2005 Jan 26

Keywords

Drug delivery
Gene therapy
GFP
Laser-induced stress wave

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency. / Terakawa, Mitsuhiro; Sato, Shunichi; Ogura, Makoto et al.
In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 5695, 36, 16.08.2005, p. 225-229.

Research output: Contribution to journal › Conference article › peer-review

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AU - Terakawa, Mitsuhiro

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AU - Masaki, Yoshinori

AU - Uenoyama, Maki

AU - Wakisaka, Hitoshi

AU - Ashida, Hiroshi

AU - Obara, Minoru

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AB - Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm2, but at higher fluences (>1.7 J/cm2), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.

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In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency

Abstract

Keywords

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