In vitro gene transfer to mammalian cells by the use of laser-induced stress waves: Effects of stress wave parameters, ambient temperature, and cell type

Mitsuhiro Terakawa, Shunichi Sato, Hiroshi Ashida, Kazuya Aizawa, Maki Uenoyama, Yoshinori Masaki, Minoru Obara

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Laser-mediated gene transfection has received much attention as a new method for targeted gene therapy because of the high spatial controllability of laser energy. We previously demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISWs).In the present study, we investigated the dependence of transfection efficiency on the laser irradiation conditions and hence stress wave conditions in vitro. We measured characteristics of LISWs used for gene transfection. For NIH 3T3 cells, transfection efficiency was evaluated as functions of laser fluence and number of pulses. The effect of ambient temperature was also investigated, and it was found that change in ambient temperature in a specific range resulted in drastic change in transfection efficiency for NIH 3T3 cells. Gene transfection of different types of cell lines were also demonstrated, where cellular heating increased transfection efficiency for nonmalig-nant cells, while heating decreased transfection efficiency for malignant cells.

Original languageEnglish
Article number014026
JournalJournal of Biomedical Optics
Volume11
Issue number1
DOIs
Publication statusPublished - 2006 Jan

Keywords

  • Cultured cells
  • Gene therapy
  • Laser-induced stress wave
  • Nonviral gene transfer

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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