In Vitro Generation of Stem Cell Memory-Like T Cells from Activated T Cells

Makoto Ando, Mari Ikeda, Akihiko Yoshimura, Taisuke Kondo

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Adoptive T-cell therapy is an attractive strategy for cancer immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively fight against the original tumor cells. The chimeric antigen receptor-engineered T (CAR-T) cells are also shown to be a promising therapy for hematologic malignancies. However, one of the limitations of these T-cell-based therapies is a rapid acquisition of tolerant (anergy, deletion, dysfunctional and/or exhausted) phenotypes of T cells during activation in vitro and/or after transfer in vivo. We and others found that stem cell memory T (TSCM) cells are strongly resistant against such tolerance, showing strong expansion and persistence in vivo, and provide long-lasting antitumor effects. Here we describe a protocol for the generation of phenotypically TSCM-like cells (iTSCM cells), which can be induced by simple co-culture of activated T cells with OP9 stroma cells expressing a Notch ligand. We also showed the methods of cancer immunotherapy by using NSG mice.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages127-139
Number of pages13
DOIs
Publication statusPublished - 2020 Jan 1

Publication series

NameMethods in Molecular Biology
Volume2111
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Adoptive T-cell therapy
  • Co-culture with feeder cells
  • EB virus-specific T cells
  • Notch signaling
  • NSG mice
  • Stem cell memory T cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Ando, M., Ikeda, M., Yoshimura, A., & Kondo, T. (2020). In Vitro Generation of Stem Cell Memory-Like T Cells from Activated T Cells. In Methods in Molecular Biology (pp. 127-139). (Methods in Molecular Biology; Vol. 2111). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0266-9_11