In Vitro Osteogenic Activity of Rat Bone Marrow Derived Mesenchymal Stem Cells Cultured on Transparent Hydroxyapatite Ceramics

Noriko Kotobuki, Daisuke Kawagoe, Hirotaka Fujimori, Seishi Goto, Koji Ioku, Hajime Ohgushi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Direct observation of cultured cells on various materials has benefit for assessment of fundamental cellular functions including cell attachment, spreading, proliferation and differentiation on the materials. For this purpose, we made transparent hydroxyapatite (tHA) ceramics, where cultured rat bone marrow-derived mesenchymal stem cells (rMSCs) could be visible by phase contrast microscopy immediately after plating. The seeded rMSCs adhered and proliferated on the tHA ceramics. Furtheremore, the cells cultured on the tHA ceramics were able to differentiate into osteoblasts under osteogenic conditions and showed comparable osteogenic activity to the cells cultured on tissue culture polystyrene dishes. The results suggested that cultured cells could be monitored in a time dependent manner using the tHA ceramics.

Original languageEnglish
Pages (from-to)1055-1058
Number of pages4
JournalKey Engineering Materials
Volume254-256
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Durapatite
Stem cells
Hydroxyapatite
Rats
Bone
Cells
Tissue culture
Polystyrenes
Osteoblasts
Plating
Microscopic examination

Keywords

  • Mesenchymal stem cell
  • Rat bone marrow
  • Transparent hydroxyapatite ceramic

ASJC Scopus subject areas

  • Ceramics and Composites
  • Chemical Engineering (miscellaneous)

Cite this

In Vitro Osteogenic Activity of Rat Bone Marrow Derived Mesenchymal Stem Cells Cultured on Transparent Hydroxyapatite Ceramics. / Kotobuki, Noriko; Kawagoe, Daisuke; Fujimori, Hirotaka; Goto, Seishi; Ioku, Koji; Ohgushi, Hajime.

In: Key Engineering Materials, Vol. 254-256, 2004, p. 1055-1058.

Research output: Contribution to journalArticle

Kotobuki, Noriko ; Kawagoe, Daisuke ; Fujimori, Hirotaka ; Goto, Seishi ; Ioku, Koji ; Ohgushi, Hajime. / In Vitro Osteogenic Activity of Rat Bone Marrow Derived Mesenchymal Stem Cells Cultured on Transparent Hydroxyapatite Ceramics. In: Key Engineering Materials. 2004 ; Vol. 254-256. pp. 1055-1058.
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