In Vitro Osteogenic Activity of Rat Bone Marrow Derived Mesenchymal Stem Cells Cultured on Transparent Hydroxyapatite Ceramics

Noriko Kotobuki, Daisuke Kawagoe, Hirotaka Fujimori, Seishi Goto, Koji Loku, Hajime Ohgushi

Research output: Contribution to journalConference article

9 Citations (Scopus)

Abstract

Direct observation of cultured cells on various materials has benefit for assessment of fundamental cellular functions including cell attachment, spreading, proliferation and differentiation on the materials. For this purpose, we made transparent hydroxyapatite (tHA) ceramics, where cultured rat bone marrow-derived mesenchymal stem cells (rMSCs) could be visible by phase contrast microscopy immediately after plating. The seeded rMSCs adhered and proliferated on the tHA ceramics. Furtheremore, the cells cultured on the tHA ceramics were able to differentiate into osteoblasts under osteogenic conditions and showed comparable osteogenic activity to the cells cultured on tissue culture polystyrene dishes. The results suggested that cultured cells could be monitored in a time dependent manner using the tHA ceramics.

Original languageEnglish
Pages (from-to)1055-1058
Number of pages4
JournalKey Engineering Materials
Volume254-256
Publication statusPublished - 2004 Jan 1
EventThe Annual Meeting of the International Society for Ceramics in Medicine - Porto, Portugal
Duration: 2003 Nov 62003 Nov 9

Keywords

  • Mesenchymal stem cell
  • Rat bone marrow
  • Transparent hydroxyapatite ceramic

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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