In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus

Ken Ishii, Masayuki Amagai

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)


Patients with pemphigus have circulating anti-desmoglein (Dsg) 1 and/or Dsg3 autoantibodies that induce blister formation on the skin and mucous membrane. We describe here two assays that measure the pathogenic strength of autoantibodies in blister formation: an in vitro dissociation assay using primary human epidermal keratinocytes to assess pathogenicity of anti-Dsg3 autoantibodies, and an alternative method whereby anti-Dsg3 and Dsg1 autoantibodies are injected into organ-cultured human skin specimen.

Original languageEnglish
Title of host publicationMolecular Dermatology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages7
ISBN (Print)9781627032261
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Autoantibody
  • Desmoglein
  • Desmosomes
  • Intercellular adhesion
  • Pemphigus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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