In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus

Ken Ishii, Masayuki Amagai

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Patients with pemphigus have circulating anti-desmoglein (Dsg) 1 and/or Dsg3 autoantibodies that induce blister formation on the skin and mucous membrane. We describe here two assays that measure the pathogenic strength of autoantibodies in blister formation: an in vitro dissociation assay using primary human epidermal keratinocytes to assess pathogenicity of anti-Dsg3 autoantibodies, and an alternative method whereby anti-Dsg3 and Dsg1 autoantibodies are injected into organ-cultured human skin specimen.

Original languageEnglish
Title of host publicationMolecular Dermatology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages219-225
Number of pages7
ISBN (Print)9781627032261
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume961
ISSN (Print)1064-3745

Keywords

  • Autoantibody
  • Desmoglein
  • Desmosomes
  • Intercellular adhesion
  • Pemphigus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Ishii, K., & Amagai, M. (2013). In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus. In Molecular Dermatology: Methods and Protocols (pp. 219-225). (Methods in Molecular Biology; Vol. 961). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-227-8_13