@inbook{c85f1e73deff414cbb84fd4fc47a28bc,
title = "In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus",
abstract = "Patients with pemphigus have circulating anti-desmoglein (Dsg) 1 and/or Dsg3 autoantibodies that induce blister formation on the skin and mucous membrane. We describe here two assays that measure the pathogenic strength of autoantibodies in blister formation: an in vitro dissociation assay using primary human epidermal keratinocytes to assess pathogenicity of anti-Dsg3 autoantibodies, and an alternative method whereby anti-Dsg3 and Dsg1 autoantibodies are injected into organ-cultured human skin specimen.",
keywords = "Autoantibody, Desmoglein, Desmosomes, Intercellular adhesion, Pemphigus",
author = "Ken Ishii and Masayuki Amagai",
note = "Copyright: Copyright 2015 Elsevier B.V., All rights reserved.",
year = "2013",
doi = "10.1007/978-1-62703-227-8_13",
language = "English",
isbn = "9781627032261",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "219--225",
booktitle = "Molecular Dermatology",
}