In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus.

Ken Ishii, Masayuki Amagai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Patients with pemphigus have circulating anti-desmoglein (Dsg) 1 and/or Dsg3 autoantibodies that induce blister formation on the skin and mucous membrane. We describe here two assays that measure the pathogenic strength of autoantibodies in blister formation: an in vitro dissociation assay using primary human epidermal keratinocytes to assess pathogenicity of anti-Dsg3 autoantibodies, and an alternative method whereby anti-Dsg3 and Dsg1 autoantibodies are injected into organ-cultured human skin specimen.

Original languageEnglish
Pages (from-to)219-225
Number of pages7
JournalMethods in molecular biology (Clifton, N.J.)
Volume961
DOIs
Publication statusPublished - 2013

Fingerprint

Desmogleins
Pemphigus
Autoantibodies
Virulence
Blister
Desmoglein 1
Skin
Keratinocytes
Mucous Membrane
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus. / Ishii, Ken; Amagai, Masayuki.

In: Methods in molecular biology (Clifton, N.J.), Vol. 961, 2013, p. 219-225.

Research output: Contribution to journalArticle

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