In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysates.

Akira Ishizuka, Kuniaki Saito, Mikiko C. Siomi, Haruhiko Siomi

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

Recent studies have shown that the microRNA (miRNA) pathway is an evolutionarily conserved endogenous pathway that is important for normal development. Mature miRNAs are excised from precursors in a stepwise process and subsequently incorporated into an RNA-induced silencing complex (RISC), which mediates either cleavage of the target messenger RNA (mRNA) or translational repression, depending on the complementarity between the miRNA and its target mRNA. In this chapter, we describe in vitro precursor (pre)-miRNA processing assays using Drosophila Schneider-2 (S2) cell lysates and immunopurified materials.

Original languageEnglish
Pages (from-to)277-286
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume342
Publication statusPublished - 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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