IN vitro production of autoantibodies to u1 ribonucleoproteins by peripheral blood mononuclear cells from patients with connective tissue diseases

Yasuo Ohosone, Masashi Akizuki, Michito Hirakata, Minoru Satoh, Hajime Yamagata, Mitsuo Homma

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Antibodies to U1 ribonucleoproteins produced by peripheral blood mononuclear cells from patients with connective tissue diseases were measured by a sensitive enzyme‐linked immunosorbent assay and by immunoblotting. Mononuclear cells from patients with high‐titer serum antibodies spontaneously started secreting IgG anti—U1 RNP antibodies on the second day after culture. The amount of anti—U1 RNP in the culture supernatants reached maximum level on day 8. The most effective production of anti—U1 RNP by B cells was observed when they were cultured in the presence of T cells and adherent cells. Mononuclear cells from patients without anti—U1 RNP antibodies or from normal subjects did not produce a measurable amount of anti—U1 RNP. Treatment of mononuclear cells by cycloheximide resulted in complete inhibition of anti—U1 RNP secretion, which indicates that antibody in the culture supernatants reflects the active biologic phenomenon in vitro. The methods described should be useful in the study of the cellular mechanisms involved in antinu‐clear antibody production of connective tissue diseases.

Original languageEnglish
Pages (from-to)1343-1350
Number of pages8
JournalArthritis & Rheumatism
Volume29
Issue number11
DOIs
Publication statusPublished - 1986 Nov

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

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