@inbook{28b43c3e0259463b82f4914df491d853,
title = "In vitro RNA cleavage assay for argonaute-family proteins",
abstract = "Recent studies have revealed that Argonaute proteins are crucial components of the RNA-induced silencing complexes (RISCs) that direct both small interfering RNA (siRNA)- and microRNA (miRNA)-mediated gene silencing. Full complementarity between the small RNA and its target messenger RNA (mRNA) results in RISC-mediated cleavage ({"}Slicing{"}) of the target mRNA. A subset of Argonaute proteins directly contributes to the target cleavage ({"}Slicer{"}) activity of the RISC. We describe (in vitro) Slicer assays using endogenous Argonaute protein immunopurified from animal cells and recombinant Argonaute protein produced in and purified from Escherichia coli.",
keywords = "Argonaute, Drosophila, RISC, RNAi, Slicer",
author = "Keita Miyoshi and Hiroshi Uejima and Tomoko Nagami-Okada and Haruhiko Siomi and Siomi, {Mikiko C.}",
year = "2008",
doi = "10.1007/978-1-59745-191-8_3",
language = "English",
isbn = "9781588298744",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "29--43",
booktitle = "RNAi",
}