In Vivo 3D Localized 13C Spectroscopy Using Modified INEPT and DEPT

H. Watanabe, Y. Ishihara, K. Okamoto, Koichi Oshio, T. Kanamatsu, Y. Tsukada

Research output: Contribution to journal › Article

13 Citations (Scopus)

Abstract

The 3D localized 13C spectroscopy methods LINEPT and LODEPT, which are modifications of INEPT and DEPT, are proposed. As long as a 13C inversion pulse (180-degree pulse) is applied at 1/(4J) before the proton echo time in LINEPT and a 13C excitation pulse (90-degree pulse) is applied at 1/(2J) before the proton echo time in LODEPT, the proton echo time can be set to any value longer than 1J(2J) in LINEPT and longer than 1/J in LODEPT. As a result, the proton and the 13C pulses can be applied separately and these proton pulses can be made slice-selective pulses. These localization features of LINEPT and LODEPT were evaluated using a phantom consisting of a cylinder filled with ethanol placed inside another cylinder filled with oil, and localized ethanol spectra could be obtained. In vivo 3D localized 13C spectra from the brain of a monkey could be obtained using decoupled LINEPT, and glutamate C-4 appeared directly after the administration of glucose C-1, followed by the appearance of glutamate C-2, C-3 and glutamine C-2, C-3, C-4.

Original languageEnglish
Pages (from-to)214-222
Number of pages9
JournalJournal of Magnetic Resonance
Volume134
Issue number2
Publication statusPublished - 1998 Oct
Externally publishedYes

Fingerprint

Protons
Spectrum Analysis
Spectroscopy
pulses
spectroscopy
protons
echoes
glutamates
Glutamic Acid
Ethanol
ethyl alcohol
Glutamine
glutamine
monkeys
Haplorhini
Brain
Oils
glucose
Glucose
brain

Keywords

  • C-MRS
  • DEPT
  • In vivo
  • INEPT
  • Localization

ASJC Scopus subject areas

  • Molecular Biology
  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Cite this

Watanabe, H., Ishihara, Y., Okamoto, K., Oshio, K., Kanamatsu, T., & Tsukada, Y. (1998). In Vivo 3D Localized 13C Spectroscopy Using Modified INEPT and DEPT. Journal of Magnetic Resonance, 134(2), 214-222.

In Vivo 3D Localized 13C Spectroscopy Using Modified INEPT and DEPT. / Watanabe, H.; Ishihara, Y.; Okamoto, K.; Oshio, Koichi; Kanamatsu, T.; Tsukada, Y.

In: Journal of Magnetic Resonance, Vol. 134, No. 2, 10.1998, p. 214-222.

Research output: Contribution to journal › Article

Watanabe, H, Ishihara, Y, Okamoto, K, Oshio, K, Kanamatsu, T & Tsukada, Y 1998, 'In Vivo 3D Localized 13C Spectroscopy Using Modified INEPT and DEPT', Journal of Magnetic Resonance, vol. 134, no. 2, pp. 214-222.
Watanabe H, Ishihara Y, Okamoto K, Oshio K, Kanamatsu T, Tsukada Y. In Vivo 3D Localized 13C Spectroscopy Using Modified INEPT and DEPT. Journal of Magnetic Resonance. 1998 Oct;134(2):214-222.
Watanabe, H. ; Ishihara, Y. ; Okamoto, K. ; Oshio, Koichi ; Kanamatsu, T. ; Tsukada, Y. / In Vivo 3D Localized 13C Spectroscopy Using Modified INEPT and DEPT. In: Journal of Magnetic Resonance. 1998 ; Vol. 134, No. 2. pp. 214-222.
@article{8b1b01b686dd4315bebf7d81444759b4,
title = "In Vivo 3D Localized 13C Spectroscopy Using Modified INEPT and DEPT",
abstract = "The 3D localized 13C spectroscopy methods LINEPT and LODEPT, which are modifications of INEPT and DEPT, are proposed. As long as a 13C inversion pulse (180-degree pulse) is applied at 1/(4J) before the proton echo time in LINEPT and a 13C excitation pulse (90-degree pulse) is applied at 1/(2J) before the proton echo time in LODEPT, the proton echo time can be set to any value longer than 1J(2J) in LINEPT and longer than 1/J in LODEPT. As a result, the proton and the 13C pulses can be applied separately and these proton pulses can be made slice-selective pulses. These localization features of LINEPT and LODEPT were evaluated using a phantom consisting of a cylinder filled with ethanol placed inside another cylinder filled with oil, and localized ethanol spectra could be obtained. In vivo 3D localized 13C spectra from the brain of a monkey could be obtained using decoupled LINEPT, and glutamate C-4 appeared directly after the administration of glucose C-1, followed by the appearance of glutamate C-2, C-3 and glutamine C-2, C-3, C-4.",
keywords = "C-MRS, DEPT, In vivo, INEPT, Localization",
author = "H. Watanabe and Y. Ishihara and K. Okamoto and Koichi Oshio and T. Kanamatsu and Y. Tsukada",
year = "1998",
month = "10",
language = "English",
volume = "134",
pages = "214--222",
journal = "Journal of Magnetic Resonance",
issn = "1090-7807",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - In Vivo 3D Localized 13C Spectroscopy Using Modified INEPT and DEPT

AU - Watanabe, H.

AU - Ishihara, Y.

AU - Okamoto, K.

AU - Oshio, Koichi

AU - Kanamatsu, T.

AU - Tsukada, Y.

PY - 1998/10

Y1 - 1998/10

N2 - The 3D localized 13C spectroscopy methods LINEPT and LODEPT, which are modifications of INEPT and DEPT, are proposed. As long as a 13C inversion pulse (180-degree pulse) is applied at 1/(4J) before the proton echo time in LINEPT and a 13C excitation pulse (90-degree pulse) is applied at 1/(2J) before the proton echo time in LODEPT, the proton echo time can be set to any value longer than 1J(2J) in LINEPT and longer than 1/J in LODEPT. As a result, the proton and the 13C pulses can be applied separately and these proton pulses can be made slice-selective pulses. These localization features of LINEPT and LODEPT were evaluated using a phantom consisting of a cylinder filled with ethanol placed inside another cylinder filled with oil, and localized ethanol spectra could be obtained. In vivo 3D localized 13C spectra from the brain of a monkey could be obtained using decoupled LINEPT, and glutamate C-4 appeared directly after the administration of glucose C-1, followed by the appearance of glutamate C-2, C-3 and glutamine C-2, C-3, C-4.

AB - The 3D localized 13C spectroscopy methods LINEPT and LODEPT, which are modifications of INEPT and DEPT, are proposed. As long as a 13C inversion pulse (180-degree pulse) is applied at 1/(4J) before the proton echo time in LINEPT and a 13C excitation pulse (90-degree pulse) is applied at 1/(2J) before the proton echo time in LODEPT, the proton echo time can be set to any value longer than 1J(2J) in LINEPT and longer than 1/J in LODEPT. As a result, the proton and the 13C pulses can be applied separately and these proton pulses can be made slice-selective pulses. These localization features of LINEPT and LODEPT were evaluated using a phantom consisting of a cylinder filled with ethanol placed inside another cylinder filled with oil, and localized ethanol spectra could be obtained. In vivo 3D localized 13C spectra from the brain of a monkey could be obtained using decoupled LINEPT, and glutamate C-4 appeared directly after the administration of glucose C-1, followed by the appearance of glutamate C-2, C-3 and glutamine C-2, C-3, C-4.

KW - C-MRS

KW - DEPT

KW - In vivo

KW - INEPT

KW - Localization

UR - http://www.scopus.com/inward/record.url?scp=0032186264&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032186264&partnerID=8YFLogxK

M3 - Article

C2 - 9761697

AN - SCOPUS:0032186264

VL - 134

SP - 214

EP - 222

JO - Journal of Magnetic Resonance

JF - Journal of Magnetic Resonance

SN - 1090-7807

IS - 2

ER -

Access to Document