In vivo assessment of cancerous tumors using boron doped diamond microelectrode

Stéphane Fierro, Momoko Yoshikawa, Osamu Nagano, Kenji Yoshimi, Hideyuki Saya, Yasuaki Einaga

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The in vitro and in vivo electrochemical detection of the reduced form of glutathione (L-γ-glutamyl-L-cysteinyl-glycine, GSH) using boron doped diamond (BDD) microelectrode for potential application in the assessment of cancerous tumors is presented. Accurate calibration curve for the determination of GSH could be obtained by the in vitro electrochemical measurements. Additionally, it was shown that it was possible to separate the detection of GSH from the oxidized form of glutathione (GSSG) using chronoamperometry measurements. In vivo GSH detection measurements have been performed in human cancer cells inoculated in immunodeficient mice. These measurements have shown that the difference of GSH level between cancerous and normal tissues can be detected. Moreover, GSH detection measurements carried out before and after X-ray irradiation have proved that it is possible to assess in vivo the decrease in GSH concentration in the tumor after a specific treatment.

Original languageEnglish
Article number901
JournalScientific reports
Volume2
DOIs
Publication statusPublished - 2012 Dec 14

ASJC Scopus subject areas

  • General

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