In Vivo Confocal Microscopy Evaluation of Ocular Surface with Graft-Versus-Host Disease-Related Dry Eye Disease

Jingliang He, Yoko Ogawa, Shin Mukai, Yumiko Saijo-Ban, Mizuka Kamoi, Miki Uchino, Mio Yamane, Nobuhiro Ozawa, Masaki Fukui, Takehiko Mori, Shinichiro Okamoto, Kazuo Tsubota

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Dry eye disease (DED) is often elicited by graft-versus-host disease (GVHD), an extensive complication of hematopoietic stem cell transplantation (HSCT). To unravel the mechanism of this type of DED, in vivo confocal microscopy (IVCM) was used to investigate alterations in the state of the sub-basal nerves, dendritic cells (DCs) and globular immune cells (GICs) in the central cornea and limbal epithelia. In this study, we examined 12 HSCT recipients with GVHD-caused DED and 10 HSCT recipients without GVHD-associated DED and evaluated the clinical parameters in the 2 groups. Analysis of the central cornea and limbal epithelia using IVCM was conducted to investigate the density of the corneal sub-basal nerves, DCs and GICs as well as the tortuosity and branching of the sub-basal nerves. As suggested by our data, the clinical variables in the GVHD group were significantly different from those in the non-GVHD group. Additionally, GVHD-triggered DED conceivably increased the density of DCs and GICs in the central cornea and the density of DCs in limbal epithelia and altered the morphology of the sub-basal nerves. These phenomena are presumably correlated with the degree of inflammation. Thus, our findings may be translated into non-invasive diagnostic methods that indicate the severity of inflammation on the ocular surface in HSCT recipients.

Original languageEnglish
Article number10720
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

Fingerprint

Eye Diseases
Graft vs Host Disease
Confocal Microscopy
Hematopoietic Stem Cell Transplantation
Dendritic Cells
Cornea
Epithelium
Inflammation
Neurons
Intravital Microscopy

ASJC Scopus subject areas

  • General

Cite this

In Vivo Confocal Microscopy Evaluation of Ocular Surface with Graft-Versus-Host Disease-Related Dry Eye Disease. / He, Jingliang; Ogawa, Yoko; Mukai, Shin; Saijo-Ban, Yumiko; Kamoi, Mizuka; Uchino, Miki; Yamane, Mio; Ozawa, Nobuhiro; Fukui, Masaki; Mori, Takehiko; Okamoto, Shinichiro; Tsubota, Kazuo.

In: Scientific Reports, Vol. 7, No. 1, 10720, 01.12.2017.

Research output: Contribution to journalArticle

He, Jingliang ; Ogawa, Yoko ; Mukai, Shin ; Saijo-Ban, Yumiko ; Kamoi, Mizuka ; Uchino, Miki ; Yamane, Mio ; Ozawa, Nobuhiro ; Fukui, Masaki ; Mori, Takehiko ; Okamoto, Shinichiro ; Tsubota, Kazuo. / In Vivo Confocal Microscopy Evaluation of Ocular Surface with Graft-Versus-Host Disease-Related Dry Eye Disease. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
@article{8c1d9392cb9f409ca3d6c459edc226fe,
title = "In Vivo Confocal Microscopy Evaluation of Ocular Surface with Graft-Versus-Host Disease-Related Dry Eye Disease",
abstract = "Dry eye disease (DED) is often elicited by graft-versus-host disease (GVHD), an extensive complication of hematopoietic stem cell transplantation (HSCT). To unravel the mechanism of this type of DED, in vivo confocal microscopy (IVCM) was used to investigate alterations in the state of the sub-basal nerves, dendritic cells (DCs) and globular immune cells (GICs) in the central cornea and limbal epithelia. In this study, we examined 12 HSCT recipients with GVHD-caused DED and 10 HSCT recipients without GVHD-associated DED and evaluated the clinical parameters in the 2 groups. Analysis of the central cornea and limbal epithelia using IVCM was conducted to investigate the density of the corneal sub-basal nerves, DCs and GICs as well as the tortuosity and branching of the sub-basal nerves. As suggested by our data, the clinical variables in the GVHD group were significantly different from those in the non-GVHD group. Additionally, GVHD-triggered DED conceivably increased the density of DCs and GICs in the central cornea and the density of DCs in limbal epithelia and altered the morphology of the sub-basal nerves. These phenomena are presumably correlated with the degree of inflammation. Thus, our findings may be translated into non-invasive diagnostic methods that indicate the severity of inflammation on the ocular surface in HSCT recipients.",
author = "Jingliang He and Yoko Ogawa and Shin Mukai and Yumiko Saijo-Ban and Mizuka Kamoi and Miki Uchino and Mio Yamane and Nobuhiro Ozawa and Masaki Fukui and Takehiko Mori and Shinichiro Okamoto and Kazuo Tsubota",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-10237-w",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - In Vivo Confocal Microscopy Evaluation of Ocular Surface with Graft-Versus-Host Disease-Related Dry Eye Disease

AU - He, Jingliang

AU - Ogawa, Yoko

AU - Mukai, Shin

AU - Saijo-Ban, Yumiko

AU - Kamoi, Mizuka

AU - Uchino, Miki

AU - Yamane, Mio

AU - Ozawa, Nobuhiro

AU - Fukui, Masaki

AU - Mori, Takehiko

AU - Okamoto, Shinichiro

AU - Tsubota, Kazuo

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Dry eye disease (DED) is often elicited by graft-versus-host disease (GVHD), an extensive complication of hematopoietic stem cell transplantation (HSCT). To unravel the mechanism of this type of DED, in vivo confocal microscopy (IVCM) was used to investigate alterations in the state of the sub-basal nerves, dendritic cells (DCs) and globular immune cells (GICs) in the central cornea and limbal epithelia. In this study, we examined 12 HSCT recipients with GVHD-caused DED and 10 HSCT recipients without GVHD-associated DED and evaluated the clinical parameters in the 2 groups. Analysis of the central cornea and limbal epithelia using IVCM was conducted to investigate the density of the corneal sub-basal nerves, DCs and GICs as well as the tortuosity and branching of the sub-basal nerves. As suggested by our data, the clinical variables in the GVHD group were significantly different from those in the non-GVHD group. Additionally, GVHD-triggered DED conceivably increased the density of DCs and GICs in the central cornea and the density of DCs in limbal epithelia and altered the morphology of the sub-basal nerves. These phenomena are presumably correlated with the degree of inflammation. Thus, our findings may be translated into non-invasive diagnostic methods that indicate the severity of inflammation on the ocular surface in HSCT recipients.

AB - Dry eye disease (DED) is often elicited by graft-versus-host disease (GVHD), an extensive complication of hematopoietic stem cell transplantation (HSCT). To unravel the mechanism of this type of DED, in vivo confocal microscopy (IVCM) was used to investigate alterations in the state of the sub-basal nerves, dendritic cells (DCs) and globular immune cells (GICs) in the central cornea and limbal epithelia. In this study, we examined 12 HSCT recipients with GVHD-caused DED and 10 HSCT recipients without GVHD-associated DED and evaluated the clinical parameters in the 2 groups. Analysis of the central cornea and limbal epithelia using IVCM was conducted to investigate the density of the corneal sub-basal nerves, DCs and GICs as well as the tortuosity and branching of the sub-basal nerves. As suggested by our data, the clinical variables in the GVHD group were significantly different from those in the non-GVHD group. Additionally, GVHD-triggered DED conceivably increased the density of DCs and GICs in the central cornea and the density of DCs in limbal epithelia and altered the morphology of the sub-basal nerves. These phenomena are presumably correlated with the degree of inflammation. Thus, our findings may be translated into non-invasive diagnostic methods that indicate the severity of inflammation on the ocular surface in HSCT recipients.

UR - http://www.scopus.com/inward/record.url?scp=85028928436&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028928436&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-10237-w

DO - 10.1038/s41598-017-10237-w

M3 - Article

C2 - 28878217

AN - SCOPUS:85028928436

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 10720

ER -