Abstract
Two-gene vectors with positive or positive-negative drug-selectable markers enable the expansion or elimination of gentetically modified cells in vivo. We have established a bicistronic retroviral vector system which utilizes an internal ribosome entry site (IRES) to co-express two independent genes with high efficiency. As a positive-negative (suicide) marker, Herpes simplex virus thymidine kinase was co-expressed with the human multidrug resistance gene, MDR1. Using this vector, almost all the MDR1-transduced cells showed hypersensitivity to a nucleoside analog, ganciclovir. As a dominant selectable marker, the MDR1 gene was co-expressed with α-galactosidase A for the model of gene therapy of Fabry disease. Vincristine selection efficiently enhanced the population of transduced cells expressing the second non-selectable genes. These drug-selectable retroviral vectors could be applicable to the therapy of many diseases.
Original language | English |
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Pages (from-to) | 552-556 |
Number of pages | 5 |
Journal | Leukemia |
Volume | 11 |
Issue number | SUPPL. 3 |
Publication status | Published - 1997 Jan 1 |
Externally published | Yes |
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research