In vivo evaluation of ocular demodicosis using laser scanning confocal microscopy

Takashi Kojima, Reiko Ishida, Enrique Adan Sato, Tetsuya Kawakita, Osama M A Ibrahim, Yukihiro Matsumoto, Minako Kaido, Murat Dogru, Kazuo Tsubota

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

PURPOSE. To investigate the applicability of in vivo laser scanning confocal microscopy in the diagnosis and follow-up of ocular demodicosis infestation in a prospective controlled study. METHODS. Fifteen right eyes of 15 patients with blepharitis associated with cylindrical dandruff (10 males, 5 females; mean age: 62.9 ± 9 years) and eight right eyes of eight age- and sex-matched control subjects underwent HRTII/RCM, evaluation of ocular symptom scores, tear function tests including vital stainings, Schirmer test, and tear clearance test, and evaluation of mite numbers in the eyelids. RESULTS. In vivo confocal microscopy effectively disclosed the mites in the terminal bulbs of the eyelashes, which were not observed after treatment. Eyelids with demodicosis infestation showed marked inflammatory infiltrates around the meibomian glands and conjunctiva, which cleared with tea tree oil treatment. CONCLUSIONS. Laser scanning confocal microscopy seems to be an efficient noninvasive tool in the diagnosis and follow-up of ocular demodicosis infestation.

Original languageEnglish
Pages (from-to)565-569
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume52
Issue number1
DOIs
Publication statusPublished - 2011 Jan

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Confocal Microscopy
Mites
Eyelids
Tears
Tea Tree Oil
Dandruff
Blepharitis
Meibomian Glands
Eyelashes
Symptom Assessment
Conjunctiva
Prospective Studies
Staining and Labeling
Therapeutics

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

In vivo evaluation of ocular demodicosis using laser scanning confocal microscopy. / Kojima, Takashi; Ishida, Reiko; Sato, Enrique Adan; Kawakita, Tetsuya; Ibrahim, Osama M A; Matsumoto, Yukihiro; Kaido, Minako; Dogru, Murat; Tsubota, Kazuo.

In: Investigative Ophthalmology and Visual Science, Vol. 52, No. 1, 01.2011, p. 565-569.

Research output: Contribution to journalArticle

Kojima, T, Ishida, R, Sato, EA, Kawakita, T, Ibrahim, OMA, Matsumoto, Y, Kaido, M, Dogru, M & Tsubota, K 2011, 'In vivo evaluation of ocular demodicosis using laser scanning confocal microscopy', Investigative Ophthalmology and Visual Science, vol. 52, no. 1, pp. 565-569. https://doi.org/10.1167/iovs.10-5477
Kojima, Takashi ; Ishida, Reiko ; Sato, Enrique Adan ; Kawakita, Tetsuya ; Ibrahim, Osama M A ; Matsumoto, Yukihiro ; Kaido, Minako ; Dogru, Murat ; Tsubota, Kazuo. / In vivo evaluation of ocular demodicosis using laser scanning confocal microscopy. In: Investigative Ophthalmology and Visual Science. 2011 ; Vol. 52, No. 1. pp. 565-569.
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