In vivo imaging in humanized mice

Hirotaka Masuda, H. J. Okano, Tetsuo Maruyama, Y. Yoshimura, Hideyuki Okano, Y. Matsuzaki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The radiological modalities that are currently utilized as critical components in clinical medicine have also been adapted to small-animal imaging, among which are ultrasound imaging, X-ray computerized tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET), and singlephoton emission computed tomography (SPECT). Optical imaging techniques such as bioluminescence imaging (BLI) and fluorescence imaging (FLI) are approaches that are commonly used in small animals. Longitudinal surveys of living (i.e., nonsacrificed) animal models with these modalities provide some clues for the development of clinical applications. The techniques are absolutely essential for translational research. However, there are currently few tools available with sufficient spatial or temporal resolution ideal for all experimental studies. In this chapter, we provide a rationale and techniques for visualizing target cells in living small animals and an overview of the advantages and limitations of current imaging technology. Finally, we introduce a humanized mouse and a novel in vivo imaging system that we have developed. We also discuss real-time observations of reconstructs and clinical manifestations.

Original languageEnglish
Pages (from-to)179-196
Number of pages18
JournalCurrent Topics in Microbiology and Immunology
Volume324
DOIs
Publication statusPublished - 2008

Fingerprint

Optical Imaging
Emission-Computed Tomography
Translational Medical Research
X Ray Computed Tomography
Clinical Medicine
Positron-Emission Tomography
Longitudinal Studies
Ultrasonography
Animal Models
Magnetic Resonance Imaging
Technology

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology (medical)
  • Immunology
  • Microbiology
  • Immunology and Microbiology(all)

Cite this

In vivo imaging in humanized mice. / Masuda, Hirotaka; Okano, H. J.; Maruyama, Tetsuo; Yoshimura, Y.; Okano, Hideyuki; Matsuzaki, Y.

In: Current Topics in Microbiology and Immunology, Vol. 324, 2008, p. 179-196.

Research output: Contribution to journalArticle

Masuda, Hirotaka ; Okano, H. J. ; Maruyama, Tetsuo ; Yoshimura, Y. ; Okano, Hideyuki ; Matsuzaki, Y. / In vivo imaging in humanized mice. In: Current Topics in Microbiology and Immunology. 2008 ; Vol. 324. pp. 179-196.
@article{a9c7c7ea6b85450a909b6375b91a567e,
title = "In vivo imaging in humanized mice",
abstract = "The radiological modalities that are currently utilized as critical components in clinical medicine have also been adapted to small-animal imaging, among which are ultrasound imaging, X-ray computerized tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET), and singlephoton emission computed tomography (SPECT). Optical imaging techniques such as bioluminescence imaging (BLI) and fluorescence imaging (FLI) are approaches that are commonly used in small animals. Longitudinal surveys of living (i.e., nonsacrificed) animal models with these modalities provide some clues for the development of clinical applications. The techniques are absolutely essential for translational research. However, there are currently few tools available with sufficient spatial or temporal resolution ideal for all experimental studies. In this chapter, we provide a rationale and techniques for visualizing target cells in living small animals and an overview of the advantages and limitations of current imaging technology. Finally, we introduce a humanized mouse and a novel in vivo imaging system that we have developed. We also discuss real-time observations of reconstructs and clinical manifestations.",
author = "Hirotaka Masuda and Okano, {H. J.} and Tetsuo Maruyama and Y. Yoshimura and Hideyuki Okano and Y. Matsuzaki",
year = "2008",
doi = "10.1007/978-3-540-75647-7_12",
language = "English",
volume = "324",
pages = "179--196",
journal = "Current Topics in Microbiology and Immunology",
issn = "0070-217X",
publisher = "Springer Verlag",

}

TY - JOUR

T1 - In vivo imaging in humanized mice

AU - Masuda, Hirotaka

AU - Okano, H. J.

AU - Maruyama, Tetsuo

AU - Yoshimura, Y.

AU - Okano, Hideyuki

AU - Matsuzaki, Y.

PY - 2008

Y1 - 2008

N2 - The radiological modalities that are currently utilized as critical components in clinical medicine have also been adapted to small-animal imaging, among which are ultrasound imaging, X-ray computerized tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET), and singlephoton emission computed tomography (SPECT). Optical imaging techniques such as bioluminescence imaging (BLI) and fluorescence imaging (FLI) are approaches that are commonly used in small animals. Longitudinal surveys of living (i.e., nonsacrificed) animal models with these modalities provide some clues for the development of clinical applications. The techniques are absolutely essential for translational research. However, there are currently few tools available with sufficient spatial or temporal resolution ideal for all experimental studies. In this chapter, we provide a rationale and techniques for visualizing target cells in living small animals and an overview of the advantages and limitations of current imaging technology. Finally, we introduce a humanized mouse and a novel in vivo imaging system that we have developed. We also discuss real-time observations of reconstructs and clinical manifestations.

AB - The radiological modalities that are currently utilized as critical components in clinical medicine have also been adapted to small-animal imaging, among which are ultrasound imaging, X-ray computerized tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET), and singlephoton emission computed tomography (SPECT). Optical imaging techniques such as bioluminescence imaging (BLI) and fluorescence imaging (FLI) are approaches that are commonly used in small animals. Longitudinal surveys of living (i.e., nonsacrificed) animal models with these modalities provide some clues for the development of clinical applications. The techniques are absolutely essential for translational research. However, there are currently few tools available with sufficient spatial or temporal resolution ideal for all experimental studies. In this chapter, we provide a rationale and techniques for visualizing target cells in living small animals and an overview of the advantages and limitations of current imaging technology. Finally, we introduce a humanized mouse and a novel in vivo imaging system that we have developed. We also discuss real-time observations of reconstructs and clinical manifestations.

UR - http://www.scopus.com/inward/record.url?scp=44449113727&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44449113727&partnerID=8YFLogxK

U2 - 10.1007/978-3-540-75647-7_12

DO - 10.1007/978-3-540-75647-7_12

M3 - Article

VL - 324

SP - 179

EP - 196

JO - Current Topics in Microbiology and Immunology

JF - Current Topics in Microbiology and Immunology

SN - 0070-217X

ER -