TY - JOUR
T1 - In vivo imaging of advanced glycation end products (AGEs) of albumin
T2 - First observations of significantly reduced clearance and liver deposition properties in mice
AU - Tsutsui, Ayumi
AU - Ogura, Akihiro
AU - Tahara, Tsuyoshi
AU - Nozaki, Satoshi
AU - Urano, Sayaka
AU - Hara, Mitsuko
AU - Kojima, Soichi
AU - Kurbangalieva, Almira
AU - Onoe, Hirotaka
AU - Watanabe, Yasuyoshi
AU - Taniguchi, Naoyuki
AU - Tanaka, Katsunori
N1 - Funding Information:
This work was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (22651081, 23681047, 25560410, and 26560438), a Research Grant from the Mizutani Foundation for Glycoscience, MEXT Grants-in-Aid for Scientific Research on Innovative Areas (No. 26102743 and 15H05843), and an AstraZeneca R&D Grant. This work was also performed with the support of the Russian Government Program for Competitive Growth, granted to the Kazan Federal University.
Publisher Copyright:
© 2016 The Royal Society of Chemistry.
PY - 2016
Y1 - 2016
N2 - Advanced glycation end products (AGEs) are associated with various diseases, especially during aging and the development of diabetes and uremia. To better understand these biological processes, investigation of the in vivo kinetics of AGEs, i.e., analysis of trafficking and clearance properties, was carried out by molecular imaging. Following the preparation of Cy7.5-labeled AGE-albumin and intravenous injection in BALB/cA-nu/nu mice, noninvasive fluorescence kinetics analysis was performed. In vivo imaging and fluorescence microscopy analysis revealed that non-enzymatic AGEs were smoothly captured by scavenger cells in the liver, i.e., Kupffer and other sinusoidal cells, but were unable to be properly cleared from the body. Overall, these results highlight an important link between AGEs and various disorders associated with them, which may serve as a platform for future research to better understand the processes and mechanisms of these disorders.
AB - Advanced glycation end products (AGEs) are associated with various diseases, especially during aging and the development of diabetes and uremia. To better understand these biological processes, investigation of the in vivo kinetics of AGEs, i.e., analysis of trafficking and clearance properties, was carried out by molecular imaging. Following the preparation of Cy7.5-labeled AGE-albumin and intravenous injection in BALB/cA-nu/nu mice, noninvasive fluorescence kinetics analysis was performed. In vivo imaging and fluorescence microscopy analysis revealed that non-enzymatic AGEs were smoothly captured by scavenger cells in the liver, i.e., Kupffer and other sinusoidal cells, but were unable to be properly cleared from the body. Overall, these results highlight an important link between AGEs and various disorders associated with them, which may serve as a platform for future research to better understand the processes and mechanisms of these disorders.
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U2 - 10.1039/c6ob00098c
DO - 10.1039/c6ob00098c
M3 - Article
C2 - 26932508
AN - SCOPUS:84975215497
VL - 14
SP - 5755
EP - 5760
JO - Organic and Biomolecular Chemistry
JF - Organic and Biomolecular Chemistry
SN - 1477-0520
IS - 24
ER -
