In Vivo Label-Free Observation of Tumor-Related Blood Vessels in Small Animals Using a Newly Designed Photoacoustic 3D Imaging System

Yasufumi Asao, Kenichi Nagae, Koichi Miyasaka, Hiroyuki Sekiguchi, Sadakazu Aiso, Shigeaki Watanabe, Marika Sato, Shinae Kizaka-Kondoh, Yukari Nakajima, Kazuo Kishi, Takayuki Yagi

Research output: Contribution to journalArticlepeer-review

Abstract

Photoacoustic (PA) technology can be used for non-invasive imaging of blood vessels. In this paper, we report on our prototype PA imaging system with a newly designed ultrasound sensor and its visualization performance of microvascular in animal. We fabricated an experimental system for animals using a high-frequency sensor. The system has two modes: still image mode by wide scanning and moving image mode by small rotation of sensor array. Optical test target, euthanized mice and rats, and live mice were used as objects. The results of optical test target showed that the spatial resolution was about two times higher than that of our conventional prototype. The image performance in vivo was evaluated in euthanized healthy mice and rats, allowing visualization of detailed blood vessels in the liver and kidneys. In tumor-bearing mice, different results of vascular induction were shown depending on the type of tumor and the method of transplantation. By utilizing the video imaging function, we were able to observe the movement of blood vessels around the tumor. We have demonstrated the feasibility of the system as a less invasive animal experimental device, as it can acquire vascular images in animals in a non-contrast and non-invasive manner.

Original languageEnglish
Pages (from-to)96-104
Number of pages9
JournalUltrasonic Imaging
Volume44
Issue number2-3
DOIs
Publication statusPublished - 2022 May

Keywords

  • angiogenesis
  • animal imaging
  • cancer
  • motion picture
  • photoacoustic
  • three-dimension

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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