In vivo photodynamic inactivation of Psuedomonas aeruginosa in burned skin in rats

Akihiro Hirao, Shunichi Sato, Mitsuhiro Terakawa, Daizoh Saitoh, Nariyoshi Shinomiya, Hiroshi Ashida, Minoru Obara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Control of infection in wounds is critically important to avoid transition to sepsis; however, recent rise of drug-resistant bacteria makes it difficult. Thus, antimicrobial photodynamic therapy (APDT) has recently received considerable attention. In this study, we examined methylene blue (MB)-mediated photodynamic inactivation of Psuedomonas aeruginosa in rat burned skin. Two days after infection, the wound surface was contacted with a MB solution at different concentrations, and thereafter the wound was irradiated with cw 665-nm light at a constant power density of 250 mW/cm2 for different time durations. We obtained a two orders of magnitude decrease in the number of bacteria by PDT with a 2-h contact of 0.5-mM MB solution and a illumination of 480 J/cm2, demonstrating the efficacy of PDT against infection with Ps. aeruginosa in burns.

Original languageEnglish
Title of host publicationOptical Methods for Tumor Treatment and Detection
Subtitle of host publicationMechanisms and Techniques in Photodynamic Therapy XIX
DOIs
Publication statusPublished - 2010 May 3
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIX - San Francisco, CA, United States
Duration: 2010 Jan 232010 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7551
ISSN (Print)1605-7422

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIX
CountryUnited States
CitySan Francisco, CA
Period10/1/2310/1/25

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Keywords

  • Burn infection
  • In vivo antibacterial PDT
  • Methylene blue
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Hirao, A., Sato, S., Terakawa, M., Saitoh, D., Shinomiya, N., Ashida, H., & Obara, M. (2010). In vivo photodynamic inactivation of Psuedomonas aeruginosa in burned skin in rats. In Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIX [75510V] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7551). https://doi.org/10.1117/12.841495