TY - GEN
T1 - In vivo photodynamic inactivation of Psuedomonas aeruginosa in burned skin in rats
AU - Hirao, Akihiro
AU - Sato, Shunichi
AU - Terakawa, Mitsuhiro
AU - Saitoh, Daizoh
AU - Shinomiya, Nariyoshi
AU - Ashida, Hiroshi
AU - Obara, Minoru
PY - 2010/5/3
Y1 - 2010/5/3
N2 - Control of infection in wounds is critically important to avoid transition to sepsis; however, recent rise of drug-resistant bacteria makes it difficult. Thus, antimicrobial photodynamic therapy (APDT) has recently received considerable attention. In this study, we examined methylene blue (MB)-mediated photodynamic inactivation of Psuedomonas aeruginosa in rat burned skin. Two days after infection, the wound surface was contacted with a MB solution at different concentrations, and thereafter the wound was irradiated with cw 665-nm light at a constant power density of 250 mW/cm2 for different time durations. We obtained a two orders of magnitude decrease in the number of bacteria by PDT with a 2-h contact of 0.5-mM MB solution and a illumination of 480 J/cm2, demonstrating the efficacy of PDT against infection with Ps. aeruginosa in burns.
AB - Control of infection in wounds is critically important to avoid transition to sepsis; however, recent rise of drug-resistant bacteria makes it difficult. Thus, antimicrobial photodynamic therapy (APDT) has recently received considerable attention. In this study, we examined methylene blue (MB)-mediated photodynamic inactivation of Psuedomonas aeruginosa in rat burned skin. Two days after infection, the wound surface was contacted with a MB solution at different concentrations, and thereafter the wound was irradiated with cw 665-nm light at a constant power density of 250 mW/cm2 for different time durations. We obtained a two orders of magnitude decrease in the number of bacteria by PDT with a 2-h contact of 0.5-mM MB solution and a illumination of 480 J/cm2, demonstrating the efficacy of PDT against infection with Ps. aeruginosa in burns.
KW - Burn infection
KW - In vivo antibacterial PDT
KW - Methylene blue
KW - Pseudomonas aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=77951611394&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951611394&partnerID=8YFLogxK
U2 - 10.1117/12.841495
DO - 10.1117/12.841495
M3 - Conference contribution
AN - SCOPUS:77951611394
SN - 9780819479471
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optical Methods for Tumor Treatment and Detection
T2 - Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIX
Y2 - 23 January 2010 through 25 January 2010
ER -