TY - JOUR
T1 - In Vivo Real-Time Simultaneous Examination of Drug Kinetics at Two Separate Locations Using Boron-Doped Diamond Microelectrodes
AU - Hanawa, Ai
AU - Ogata, Genki
AU - Sawamura, Seishiro
AU - Asai, Kai
AU - Kanzaki, Sho
AU - Hibino, Hiroshi
AU - Einaga, Yasuaki
N1 - Funding Information:
This study was partially supported by the following research grants: JST-ACCEL (to Y.E.), Grant-in-Aid for Scientific Research A 19H00832 (to Y.E.), Grant-in-Aid for Scientific Research A 18H04062 (to H.H.), Grant-in-Aid for Scientific Research B 18H03513 (to G.O.), and Grants-in-Aid for Young Scientists B 17K17736 (S.S.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. In addition, funds were kindly provided by The Nakatani Foundation (to H.H. and Y.E.), Takeda Science Foundation (to G.O.), The Mochida Memorial Foundation for Medical and Pharmaceutical Research (to G.O.), Fukuda Foundation for Medical Technology (to G.O.), Nishinomiya Basic Research Fund, Japan (to G.O.), The Ichiro Kanehara Foundation for the Promotion of Medical Sciences and Medical Care (to G.O.), and Yamaguchi Educational and Scholarship Foundation (to G.O.).
PY - 2020/10/20
Y1 - 2020/10/20
N2 - Methylcobalamin, which is used for the clinical treatment of patients with neuropathy, can have an impact on the sensorineural components associated with the cochlea, and it is possible that the auditory threshold in a certain population of patients with deafness may be recovered. Nonetheless, it remains uncertain whether the action site of methylcobalamin is localized inside or outside the cochlea and which cellular or tissue element is targeted by the drug. In the present work, we developed a method to realize in vivo real-time simultaneous examination of the drug kinetics in two separate locations using boron-doped diamond microelectrodes. First, the analytical performance of methylcobalamin was studied and the measurement protocol was optimized in vitro. Then, the optimized protocol was applied to carry out real-time measurements inside the cochlea and the leg muscle in live guinea pigs while systemically administering methylcobalamin. The results showed that the methylcobalamin concentration in the cochlea was below the limit of detection for the microelectrodes or the drug did not reach the cochlea, whereas the compound clearly reached the leg muscle.
AB - Methylcobalamin, which is used for the clinical treatment of patients with neuropathy, can have an impact on the sensorineural components associated with the cochlea, and it is possible that the auditory threshold in a certain population of patients with deafness may be recovered. Nonetheless, it remains uncertain whether the action site of methylcobalamin is localized inside or outside the cochlea and which cellular or tissue element is targeted by the drug. In the present work, we developed a method to realize in vivo real-time simultaneous examination of the drug kinetics in two separate locations using boron-doped diamond microelectrodes. First, the analytical performance of methylcobalamin was studied and the measurement protocol was optimized in vitro. Then, the optimized protocol was applied to carry out real-time measurements inside the cochlea and the leg muscle in live guinea pigs while systemically administering methylcobalamin. The results showed that the methylcobalamin concentration in the cochlea was below the limit of detection for the microelectrodes or the drug did not reach the cochlea, whereas the compound clearly reached the leg muscle.
UR - http://www.scopus.com/inward/record.url?scp=85096717185&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85096717185&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.0c01707
DO - 10.1021/acs.analchem.0c01707
M3 - Article
AN - SCOPUS:85096717185
VL - 92
SP - 13742
EP - 13749
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 20
ER -