In vivo role of IFN-γ produced by antigen-presenting cells in early host defense against intracellular pathogens

Kazutomo Suzue, Takashi Asai, Tsutomu Takeuchi, Shigeo Koyasu

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Antigen-presenting cells (APC), including dendritic cells and macrophages, produce a large amount of interferon (IFN)-γ, a crucial cytokine for the control of infectious diseases. To elucidate the role of IFN-γ from APC in vivo, we employed cytokine receptor common γ subunit (γc) and recombination-activating gene (Rag -2 double-knockout (γc-/-(y)-Rag-2-/-) mice, which are severely impaired in IFN-γ production and are extremely susceptible to infection of intracellular pathogens including Listeria monocytogenes and Toxoplasma gondii. Adoptive transfer of IFN-γ-producing APC increased levels of serum IFN-γ and the resistance to Listeria. Although depletion of NK cells from Rag-2-/- mice slightly increased the susceptibility to bacterial infection, they are substantially more resistant than γc-/-(y)-Rag-2-/- mice, which are also devoid of all lymphoid cells. These results demonstrate that the APC-derived IFN-γ contributes to the control of infectious agents in vivo.

Original languageEnglish
Pages (from-to)2666-2675
Number of pages10
JournalEuropean Journal of Immunology
Volume33
Issue number10
DOIs
Publication statusPublished - 2003 Oct

Fingerprint

Antigen-Presenting Cells
Interferons
Listeria
Cytokine Receptors
Adoptive Transfer
Toxoplasma
Listeria monocytogenes
Bacterial Infections
Natural Killer Cells
Dendritic Cells
Genetic Recombination
Communicable Diseases
Macrophages
Lymphocytes
Cytokines
Infection
Serum
Genes

Keywords

  • Dendritic cells
  • Listeria monocytogenes
  • Macrophage
  • NK cells
  • X-linked SCID

ASJC Scopus subject areas

  • Immunology

Cite this

In vivo role of IFN-γ produced by antigen-presenting cells in early host defense against intracellular pathogens. / Suzue, Kazutomo; Asai, Takashi; Takeuchi, Tsutomu; Koyasu, Shigeo.

In: European Journal of Immunology, Vol. 33, No. 10, 10.2003, p. 2666-2675.

Research output: Contribution to journalArticle

Suzue, Kazutomo ; Asai, Takashi ; Takeuchi, Tsutomu ; Koyasu, Shigeo. / In vivo role of IFN-γ produced by antigen-presenting cells in early host defense against intracellular pathogens. In: European Journal of Immunology. 2003 ; Vol. 33, No. 10. pp. 2666-2675.
@article{5788d76741234a6480d9a46c11bc5750,
title = "In vivo role of IFN-γ produced by antigen-presenting cells in early host defense against intracellular pathogens",
abstract = "Antigen-presenting cells (APC), including dendritic cells and macrophages, produce a large amount of interferon (IFN)-γ, a crucial cytokine for the control of infectious diseases. To elucidate the role of IFN-γ from APC in vivo, we employed cytokine receptor common γ subunit (γc) and recombination-activating gene (Rag -2 double-knockout (γc-/-(y)-Rag-2-/-) mice, which are severely impaired in IFN-γ production and are extremely susceptible to infection of intracellular pathogens including Listeria monocytogenes and Toxoplasma gondii. Adoptive transfer of IFN-γ-producing APC increased levels of serum IFN-γ and the resistance to Listeria. Although depletion of NK cells from Rag-2-/- mice slightly increased the susceptibility to bacterial infection, they are substantially more resistant than γc-/-(y)-Rag-2-/- mice, which are also devoid of all lymphoid cells. These results demonstrate that the APC-derived IFN-γ contributes to the control of infectious agents in vivo.",
keywords = "Dendritic cells, Listeria monocytogenes, Macrophage, NK cells, X-linked SCID",
author = "Kazutomo Suzue and Takashi Asai and Tsutomu Takeuchi and Shigeo Koyasu",
year = "2003",
month = "10",
doi = "10.1002/eji.200323292",
language = "English",
volume = "33",
pages = "2666--2675",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "10",

}

TY - JOUR

T1 - In vivo role of IFN-γ produced by antigen-presenting cells in early host defense against intracellular pathogens

AU - Suzue, Kazutomo

AU - Asai, Takashi

AU - Takeuchi, Tsutomu

AU - Koyasu, Shigeo

PY - 2003/10

Y1 - 2003/10

N2 - Antigen-presenting cells (APC), including dendritic cells and macrophages, produce a large amount of interferon (IFN)-γ, a crucial cytokine for the control of infectious diseases. To elucidate the role of IFN-γ from APC in vivo, we employed cytokine receptor common γ subunit (γc) and recombination-activating gene (Rag -2 double-knockout (γc-/-(y)-Rag-2-/-) mice, which are severely impaired in IFN-γ production and are extremely susceptible to infection of intracellular pathogens including Listeria monocytogenes and Toxoplasma gondii. Adoptive transfer of IFN-γ-producing APC increased levels of serum IFN-γ and the resistance to Listeria. Although depletion of NK cells from Rag-2-/- mice slightly increased the susceptibility to bacterial infection, they are substantially more resistant than γc-/-(y)-Rag-2-/- mice, which are also devoid of all lymphoid cells. These results demonstrate that the APC-derived IFN-γ contributes to the control of infectious agents in vivo.

AB - Antigen-presenting cells (APC), including dendritic cells and macrophages, produce a large amount of interferon (IFN)-γ, a crucial cytokine for the control of infectious diseases. To elucidate the role of IFN-γ from APC in vivo, we employed cytokine receptor common γ subunit (γc) and recombination-activating gene (Rag -2 double-knockout (γc-/-(y)-Rag-2-/-) mice, which are severely impaired in IFN-γ production and are extremely susceptible to infection of intracellular pathogens including Listeria monocytogenes and Toxoplasma gondii. Adoptive transfer of IFN-γ-producing APC increased levels of serum IFN-γ and the resistance to Listeria. Although depletion of NK cells from Rag-2-/- mice slightly increased the susceptibility to bacterial infection, they are substantially more resistant than γc-/-(y)-Rag-2-/- mice, which are also devoid of all lymphoid cells. These results demonstrate that the APC-derived IFN-γ contributes to the control of infectious agents in vivo.

KW - Dendritic cells

KW - Listeria monocytogenes

KW - Macrophage

KW - NK cells

KW - X-linked SCID

UR - http://www.scopus.com/inward/record.url?scp=0242366613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242366613&partnerID=8YFLogxK

U2 - 10.1002/eji.200323292

DO - 10.1002/eji.200323292

M3 - Article

VL - 33

SP - 2666

EP - 2675

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 10

ER -