In vivo tracing of neural tracts in the intact and injured spinal cord of marmosets by diffusion tensor tractography

Kanehiro Fujiyoshi, Masayuki Yamada, Masaya Nakamura, Junichi Yamane, Hiroyuki Katoh, Kazuya Kitamura, Kenji Kawai, Seiji Okada, Suketaka Momoshima, Yoshiaki Toyama, Hideyuki Okano

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

In spinal cord injury, axonal disruption results in motor and sensory function impairment. The evaluation of axonal fibers is essential to assess the severity of injury and efficacy of any treatment protocol, but conventional methods such as tracer injection in brain parenchyma are highly invasive and require histological evaluation, precluding clinical applications. Previous advances in magnetic resonance imaging technology have led to the development of diffusion tensor tractography (DTT) as a potential modality to perform in vivo tracing of axonal fibers. The properties and clinical applications of DTT in the brain have been reported, but technical difficulties have limited DTT studies of the spinal cord. In this study, we report the effective use of DTT to visualize both intact and surgically disrupted spinal long tracts in adult common marmosets. To verify the feasibility of spinal cord DTT, we first performed DTT of postmortem marmosets. DTT clearly illustrated spinal projections such as the corticospinal tract and afferent fibers in control animals, and depicted the severed long tracts in the injured animals. Histology of the spinal cords in both control and injured groups were consistent with DTT findings, verifying the accuracy of DTT. We also conducted DTT in live marmosets and demonstrated that DTT can be performed in live animals to reveal in vivo nerve fiber tracing images, providing an essential tool to evaluate axonal conditions in the injured spinal cord. Taken together, these findings demonstrate the feasibility of applying DTT to preclinical and clinical studies of spinal cord injury.

Original languageEnglish
Pages (from-to)11991-11998
Number of pages8
JournalJournal of Neuroscience
Volume27
Issue number44
DOIs
Publication statusPublished - 2007 Oct 31

Fingerprint

Callithrix
Diffusion Tensor Imaging
Spinal Cord
Spinal Cord Injuries
Pyramidal Tracts
Brain
Clinical Protocols
Nerve Fibers
Histology

Keywords

  • Calmodulin-dependent protein kinase II-α
  • Common marmoset
  • Corticospinal tract
  • Diffusion tensor tractography
  • in vivo tracing
  • Magnetic resonance imaging
  • Pathway-specific DTT
  • Spinal cord injury

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

In vivo tracing of neural tracts in the intact and injured spinal cord of marmosets by diffusion tensor tractography. / Fujiyoshi, Kanehiro; Yamada, Masayuki; Nakamura, Masaya; Yamane, Junichi; Katoh, Hiroyuki; Kitamura, Kazuya; Kawai, Kenji; Okada, Seiji; Momoshima, Suketaka; Toyama, Yoshiaki; Okano, Hideyuki.

In: Journal of Neuroscience, Vol. 27, No. 44, 31.10.2007, p. 11991-11998.

Research output: Contribution to journalArticle

Fujiyoshi, Kanehiro ; Yamada, Masayuki ; Nakamura, Masaya ; Yamane, Junichi ; Katoh, Hiroyuki ; Kitamura, Kazuya ; Kawai, Kenji ; Okada, Seiji ; Momoshima, Suketaka ; Toyama, Yoshiaki ; Okano, Hideyuki. / In vivo tracing of neural tracts in the intact and injured spinal cord of marmosets by diffusion tensor tractography. In: Journal of Neuroscience. 2007 ; Vol. 27, No. 44. pp. 11991-11998.
@article{725030e8d129430fa9ef1cb6220ef806,
title = "In vivo tracing of neural tracts in the intact and injured spinal cord of marmosets by diffusion tensor tractography",
abstract = "In spinal cord injury, axonal disruption results in motor and sensory function impairment. The evaluation of axonal fibers is essential to assess the severity of injury and efficacy of any treatment protocol, but conventional methods such as tracer injection in brain parenchyma are highly invasive and require histological evaluation, precluding clinical applications. Previous advances in magnetic resonance imaging technology have led to the development of diffusion tensor tractography (DTT) as a potential modality to perform in vivo tracing of axonal fibers. The properties and clinical applications of DTT in the brain have been reported, but technical difficulties have limited DTT studies of the spinal cord. In this study, we report the effective use of DTT to visualize both intact and surgically disrupted spinal long tracts in adult common marmosets. To verify the feasibility of spinal cord DTT, we first performed DTT of postmortem marmosets. DTT clearly illustrated spinal projections such as the corticospinal tract and afferent fibers in control animals, and depicted the severed long tracts in the injured animals. Histology of the spinal cords in both control and injured groups were consistent with DTT findings, verifying the accuracy of DTT. We also conducted DTT in live marmosets and demonstrated that DTT can be performed in live animals to reveal in vivo nerve fiber tracing images, providing an essential tool to evaluate axonal conditions in the injured spinal cord. Taken together, these findings demonstrate the feasibility of applying DTT to preclinical and clinical studies of spinal cord injury.",
keywords = "Calmodulin-dependent protein kinase II-α, Common marmoset, Corticospinal tract, Diffusion tensor tractography, in vivo tracing, Magnetic resonance imaging, Pathway-specific DTT, Spinal cord injury",
author = "Kanehiro Fujiyoshi and Masayuki Yamada and Masaya Nakamura and Junichi Yamane and Hiroyuki Katoh and Kazuya Kitamura and Kenji Kawai and Seiji Okada and Suketaka Momoshima and Yoshiaki Toyama and Hideyuki Okano",
year = "2007",
month = "10",
day = "31",
doi = "10.1523/JNEUROSCI.3354-07.2007",
language = "English",
volume = "27",
pages = "11991--11998",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "44",

}

TY - JOUR

T1 - In vivo tracing of neural tracts in the intact and injured spinal cord of marmosets by diffusion tensor tractography

AU - Fujiyoshi, Kanehiro

AU - Yamada, Masayuki

AU - Nakamura, Masaya

AU - Yamane, Junichi

AU - Katoh, Hiroyuki

AU - Kitamura, Kazuya

AU - Kawai, Kenji

AU - Okada, Seiji

AU - Momoshima, Suketaka

AU - Toyama, Yoshiaki

AU - Okano, Hideyuki

PY - 2007/10/31

Y1 - 2007/10/31

N2 - In spinal cord injury, axonal disruption results in motor and sensory function impairment. The evaluation of axonal fibers is essential to assess the severity of injury and efficacy of any treatment protocol, but conventional methods such as tracer injection in brain parenchyma are highly invasive and require histological evaluation, precluding clinical applications. Previous advances in magnetic resonance imaging technology have led to the development of diffusion tensor tractography (DTT) as a potential modality to perform in vivo tracing of axonal fibers. The properties and clinical applications of DTT in the brain have been reported, but technical difficulties have limited DTT studies of the spinal cord. In this study, we report the effective use of DTT to visualize both intact and surgically disrupted spinal long tracts in adult common marmosets. To verify the feasibility of spinal cord DTT, we first performed DTT of postmortem marmosets. DTT clearly illustrated spinal projections such as the corticospinal tract and afferent fibers in control animals, and depicted the severed long tracts in the injured animals. Histology of the spinal cords in both control and injured groups were consistent with DTT findings, verifying the accuracy of DTT. We also conducted DTT in live marmosets and demonstrated that DTT can be performed in live animals to reveal in vivo nerve fiber tracing images, providing an essential tool to evaluate axonal conditions in the injured spinal cord. Taken together, these findings demonstrate the feasibility of applying DTT to preclinical and clinical studies of spinal cord injury.

AB - In spinal cord injury, axonal disruption results in motor and sensory function impairment. The evaluation of axonal fibers is essential to assess the severity of injury and efficacy of any treatment protocol, but conventional methods such as tracer injection in brain parenchyma are highly invasive and require histological evaluation, precluding clinical applications. Previous advances in magnetic resonance imaging technology have led to the development of diffusion tensor tractography (DTT) as a potential modality to perform in vivo tracing of axonal fibers. The properties and clinical applications of DTT in the brain have been reported, but technical difficulties have limited DTT studies of the spinal cord. In this study, we report the effective use of DTT to visualize both intact and surgically disrupted spinal long tracts in adult common marmosets. To verify the feasibility of spinal cord DTT, we first performed DTT of postmortem marmosets. DTT clearly illustrated spinal projections such as the corticospinal tract and afferent fibers in control animals, and depicted the severed long tracts in the injured animals. Histology of the spinal cords in both control and injured groups were consistent with DTT findings, verifying the accuracy of DTT. We also conducted DTT in live marmosets and demonstrated that DTT can be performed in live animals to reveal in vivo nerve fiber tracing images, providing an essential tool to evaluate axonal conditions in the injured spinal cord. Taken together, these findings demonstrate the feasibility of applying DTT to preclinical and clinical studies of spinal cord injury.

KW - Calmodulin-dependent protein kinase II-α

KW - Common marmoset

KW - Corticospinal tract

KW - Diffusion tensor tractography

KW - in vivo tracing

KW - Magnetic resonance imaging

KW - Pathway-specific DTT

KW - Spinal cord injury

UR - http://www.scopus.com/inward/record.url?scp=35948957080&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35948957080&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.3354-07.2007

DO - 10.1523/JNEUROSCI.3354-07.2007

M3 - Article

C2 - 17978040

AN - SCOPUS:35948957080

VL - 27

SP - 11991

EP - 11998

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 44

ER -