In vivo visualization of angiotensin II- and tubuloglomerular feedback-mediated renal vasoconstriction

T. Yamamoto, K. Hayashi, H. Matsuda, E. Kubota, H. Tanaka, Y. Ogasawara, H. Nakamoto, H. Suzuki, T. Saruta, F. Kajiya

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Background. A noninvasive technique to monitor renal microcirculation would be a useful tool for investigation of renal disease and the effects of drugs on the renal system. We have developed a novel, less invasive technique to visualize renal microcirculation in vivo using an intravital tapered-tip (1 mm ø) lens-probe (pencil lens-probe) videomicroscopy, which only requires insertion of the probe into superficial renal cortex in situ. Methods. To assess validity of this technique, the effects of angiotensin II (Ang II) and intrarenal sodium chloride loading (activator of tubuloglomerular mechanism) were examined. The renal microvasculature was successfully visualized and monitored. Results. Administration of Ang II (1, 3, 10 and 30 ng/kg/ min) produced a dose-dependent constriction of afferent and efferent arterioles in similar degrees; at 30 ng/kg/min, Ang II elicited 52 ± 3 (N = 9) and 53 ± 3% decreases in diameter (N = 9), respectively. The Ang II-induced arteriolar constriction was completely prevented by losartan, an Ang II type 1 (AT1) antagonist. The intrarenal hypertonic saline administration elicited transient increments (from 98 ± 8 to 122 ± 7 mL/ min, N = 6, P < 0.05), followed by a marked reduction in renal blood flow (RBF; 78 ± 7 mL/min, P ± 0.05). This response was accompanied by prominent constriction of afferent (from 15.0 ± 1.1 to 8.5 ± 1.1 μm, N = 6, P < 0.05), but not efferent (from 14.3 ± 1.2 to 13.8 ± 1.0 μm, N = 3) arterioles. Furthermore, this response was completely inhibited by furosemide, a tubuloglomerular feedback inhibitor. Conclusion. The intravital pencil lens-probe videomicroscopy can be a powerful tool for in vivo observation of renal microcirculation, with intact renal microvascular responses to two important renal homeostatic mechanisms, angiotensin II and tubuloglomerular feedback.

Original languageEnglish
Pages (from-to)364-369
Number of pages6
JournalKidney International
Volume60
Issue number1
DOIs
Publication statusPublished - 2001

Fingerprint

Vasoconstriction
Angiotensin II
Kidney
Microcirculation
Constriction
Lenses
Video Microscopy
Arterioles
Losartan
Renal Circulation
Furosemide
Microvessels
Sodium Chloride
Observation
Pharmaceutical Preparations

Keywords

  • Afferent arterioles
  • Efferent arterioles
  • Hemodynamics
  • Renal microcirculation
  • Tubuloglomerular feedback
  • Videomicroscopy

ASJC Scopus subject areas

  • Nephrology

Cite this

Yamamoto, T., Hayashi, K., Matsuda, H., Kubota, E., Tanaka, H., Ogasawara, Y., ... Kajiya, F. (2001). In vivo visualization of angiotensin II- and tubuloglomerular feedback-mediated renal vasoconstriction. Kidney International, 60(1), 364-369. https://doi.org/10.1046/j.1523-1755.2001.00808.x

In vivo visualization of angiotensin II- and tubuloglomerular feedback-mediated renal vasoconstriction. / Yamamoto, T.; Hayashi, K.; Matsuda, H.; Kubota, E.; Tanaka, H.; Ogasawara, Y.; Nakamoto, H.; Suzuki, H.; Saruta, T.; Kajiya, F.

In: Kidney International, Vol. 60, No. 1, 2001, p. 364-369.

Research output: Contribution to journalArticle

Yamamoto, T, Hayashi, K, Matsuda, H, Kubota, E, Tanaka, H, Ogasawara, Y, Nakamoto, H, Suzuki, H, Saruta, T & Kajiya, F 2001, 'In vivo visualization of angiotensin II- and tubuloglomerular feedback-mediated renal vasoconstriction', Kidney International, vol. 60, no. 1, pp. 364-369. https://doi.org/10.1046/j.1523-1755.2001.00808.x
Yamamoto, T. ; Hayashi, K. ; Matsuda, H. ; Kubota, E. ; Tanaka, H. ; Ogasawara, Y. ; Nakamoto, H. ; Suzuki, H. ; Saruta, T. ; Kajiya, F. / In vivo visualization of angiotensin II- and tubuloglomerular feedback-mediated renal vasoconstriction. In: Kidney International. 2001 ; Vol. 60, No. 1. pp. 364-369.
@article{e5fba6bc6a3a40a0b5bab6bf2bef17dd,
title = "In vivo visualization of angiotensin II- and tubuloglomerular feedback-mediated renal vasoconstriction",
abstract = "Background. A noninvasive technique to monitor renal microcirculation would be a useful tool for investigation of renal disease and the effects of drugs on the renal system. We have developed a novel, less invasive technique to visualize renal microcirculation in vivo using an intravital tapered-tip (1 mm {\o}) lens-probe (pencil lens-probe) videomicroscopy, which only requires insertion of the probe into superficial renal cortex in situ. Methods. To assess validity of this technique, the effects of angiotensin II (Ang II) and intrarenal sodium chloride loading (activator of tubuloglomerular mechanism) were examined. The renal microvasculature was successfully visualized and monitored. Results. Administration of Ang II (1, 3, 10 and 30 ng/kg/ min) produced a dose-dependent constriction of afferent and efferent arterioles in similar degrees; at 30 ng/kg/min, Ang II elicited 52 ± 3 (N = 9) and 53 ± 3{\%} decreases in diameter (N = 9), respectively. The Ang II-induced arteriolar constriction was completely prevented by losartan, an Ang II type 1 (AT1) antagonist. The intrarenal hypertonic saline administration elicited transient increments (from 98 ± 8 to 122 ± 7 mL/ min, N = 6, P < 0.05), followed by a marked reduction in renal blood flow (RBF; 78 ± 7 mL/min, P ± 0.05). This response was accompanied by prominent constriction of afferent (from 15.0 ± 1.1 to 8.5 ± 1.1 μm, N = 6, P < 0.05), but not efferent (from 14.3 ± 1.2 to 13.8 ± 1.0 μm, N = 3) arterioles. Furthermore, this response was completely inhibited by furosemide, a tubuloglomerular feedback inhibitor. Conclusion. The intravital pencil lens-probe videomicroscopy can be a powerful tool for in vivo observation of renal microcirculation, with intact renal microvascular responses to two important renal homeostatic mechanisms, angiotensin II and tubuloglomerular feedback.",
keywords = "Afferent arterioles, Efferent arterioles, Hemodynamics, Renal microcirculation, Tubuloglomerular feedback, Videomicroscopy",
author = "T. Yamamoto and K. Hayashi and H. Matsuda and E. Kubota and H. Tanaka and Y. Ogasawara and H. Nakamoto and H. Suzuki and T. Saruta and F. Kajiya",
year = "2001",
doi = "10.1046/j.1523-1755.2001.00808.x",
language = "English",
volume = "60",
pages = "364--369",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - In vivo visualization of angiotensin II- and tubuloglomerular feedback-mediated renal vasoconstriction

AU - Yamamoto, T.

AU - Hayashi, K.

AU - Matsuda, H.

AU - Kubota, E.

AU - Tanaka, H.

AU - Ogasawara, Y.

AU - Nakamoto, H.

AU - Suzuki, H.

AU - Saruta, T.

AU - Kajiya, F.

PY - 2001

Y1 - 2001

N2 - Background. A noninvasive technique to monitor renal microcirculation would be a useful tool for investigation of renal disease and the effects of drugs on the renal system. We have developed a novel, less invasive technique to visualize renal microcirculation in vivo using an intravital tapered-tip (1 mm ø) lens-probe (pencil lens-probe) videomicroscopy, which only requires insertion of the probe into superficial renal cortex in situ. Methods. To assess validity of this technique, the effects of angiotensin II (Ang II) and intrarenal sodium chloride loading (activator of tubuloglomerular mechanism) were examined. The renal microvasculature was successfully visualized and monitored. Results. Administration of Ang II (1, 3, 10 and 30 ng/kg/ min) produced a dose-dependent constriction of afferent and efferent arterioles in similar degrees; at 30 ng/kg/min, Ang II elicited 52 ± 3 (N = 9) and 53 ± 3% decreases in diameter (N = 9), respectively. The Ang II-induced arteriolar constriction was completely prevented by losartan, an Ang II type 1 (AT1) antagonist. The intrarenal hypertonic saline administration elicited transient increments (from 98 ± 8 to 122 ± 7 mL/ min, N = 6, P < 0.05), followed by a marked reduction in renal blood flow (RBF; 78 ± 7 mL/min, P ± 0.05). This response was accompanied by prominent constriction of afferent (from 15.0 ± 1.1 to 8.5 ± 1.1 μm, N = 6, P < 0.05), but not efferent (from 14.3 ± 1.2 to 13.8 ± 1.0 μm, N = 3) arterioles. Furthermore, this response was completely inhibited by furosemide, a tubuloglomerular feedback inhibitor. Conclusion. The intravital pencil lens-probe videomicroscopy can be a powerful tool for in vivo observation of renal microcirculation, with intact renal microvascular responses to two important renal homeostatic mechanisms, angiotensin II and tubuloglomerular feedback.

AB - Background. A noninvasive technique to monitor renal microcirculation would be a useful tool for investigation of renal disease and the effects of drugs on the renal system. We have developed a novel, less invasive technique to visualize renal microcirculation in vivo using an intravital tapered-tip (1 mm ø) lens-probe (pencil lens-probe) videomicroscopy, which only requires insertion of the probe into superficial renal cortex in situ. Methods. To assess validity of this technique, the effects of angiotensin II (Ang II) and intrarenal sodium chloride loading (activator of tubuloglomerular mechanism) were examined. The renal microvasculature was successfully visualized and monitored. Results. Administration of Ang II (1, 3, 10 and 30 ng/kg/ min) produced a dose-dependent constriction of afferent and efferent arterioles in similar degrees; at 30 ng/kg/min, Ang II elicited 52 ± 3 (N = 9) and 53 ± 3% decreases in diameter (N = 9), respectively. The Ang II-induced arteriolar constriction was completely prevented by losartan, an Ang II type 1 (AT1) antagonist. The intrarenal hypertonic saline administration elicited transient increments (from 98 ± 8 to 122 ± 7 mL/ min, N = 6, P < 0.05), followed by a marked reduction in renal blood flow (RBF; 78 ± 7 mL/min, P ± 0.05). This response was accompanied by prominent constriction of afferent (from 15.0 ± 1.1 to 8.5 ± 1.1 μm, N = 6, P < 0.05), but not efferent (from 14.3 ± 1.2 to 13.8 ± 1.0 μm, N = 3) arterioles. Furthermore, this response was completely inhibited by furosemide, a tubuloglomerular feedback inhibitor. Conclusion. The intravital pencil lens-probe videomicroscopy can be a powerful tool for in vivo observation of renal microcirculation, with intact renal microvascular responses to two important renal homeostatic mechanisms, angiotensin II and tubuloglomerular feedback.

KW - Afferent arterioles

KW - Efferent arterioles

KW - Hemodynamics

KW - Renal microcirculation

KW - Tubuloglomerular feedback

KW - Videomicroscopy

UR - http://www.scopus.com/inward/record.url?scp=0034955116&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034955116&partnerID=8YFLogxK

U2 - 10.1046/j.1523-1755.2001.00808.x

DO - 10.1046/j.1523-1755.2001.00808.x

M3 - Article

C2 - 11422773

AN - SCOPUS:0034955116

VL - 60

SP - 364

EP - 369

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 1

ER -