In Vivo visualization of oxyradical-dependent photoemission during endothelium-granulocyte interaction in microvascular beds treated with platelet-activating factor

Makoto Suematsu, Iwao Kurose, Hiroshi Asako, Soichiro Miura, Masaharu Tsuchiya

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Oxyradical-dependent chemiluminescence from granulocytes sticking to venular endothelium was successfully visualized in rat mesenteric microvascular beds treated with platelet-activating factor (PAF). Intravital photonic image intensifier microscopy revealed that topical application of PAF-acether (100 nM) caused remarkable granulocyte adherence on endothelial walls and the subsequent activation of a luminol-dependent photonic burst. Chemilumigenic sites clearly corresponded to the spatial distribution of sticking cells in post-capillary venules. The present findings thus serve as the first demonstration of an intravital oxidative burst of granulocytes on venular endothelium in PAF-induced microcirculatory disturbances.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalJournal of biochemistry
Volume106
Issue number2
DOIs
Publication statusPublished - 1989 Aug

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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