In Vivo visualization of oxyradical-dependent photoemission during endothelium-granulocyte interaction in microvascular beds treated with platelet-activating factor

Makoto Suematsu, Iwao Kurose, Hiroshi Asako, Soichiro Miura, Masaharu Tsuchiya

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Oxyradical-dependent chemiluminescence from granulocytes sticking to venular endothelium was successfully visualized in rat mesenteric microvascular beds treated with platelet-activating factor (PAF). Intravital photonic image intensifier microscopy revealed that topical application of PAF-acether (100 nM) caused remarkable granulocyte adherence on endothelial walls and the subsequent activation of a luminol-dependent photonic burst. Chemilumigenic sites clearly corresponded to the spatial distribution of sticking cells in post-capillary venules. The present findings thus serve as the first demonstration of an intravital oxidative burst of granulocytes on venular endothelium in PAF-induced microcirculatory disturbances.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalJournal of Biochemistry
Volume106
Issue number2
Publication statusPublished - 1989 Aug

Fingerprint

Endothelium
Platelets
Platelet Activating Factor
Photoemission
Granulocytes
Optics and Photonics
Visualization
Burst
Photonics
Dependent
Interaction
Image Intensifier
Luminol
Chemiluminescence
Respiratory Burst
Venules
Luminescence
Spatial Distribution
Microscopy
Spatial distribution

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

In Vivo visualization of oxyradical-dependent photoemission during endothelium-granulocyte interaction in microvascular beds treated with platelet-activating factor. / Suematsu, Makoto; Kurose, Iwao; Asako, Hiroshi; Miura, Soichiro; Tsuchiya, Masaharu.

In: Journal of Biochemistry, Vol. 106, No. 2, 08.1989, p. 355-360.

Research output: Contribution to journalArticle

Suematsu, Makoto ; Kurose, Iwao ; Asako, Hiroshi ; Miura, Soichiro ; Tsuchiya, Masaharu. / In Vivo visualization of oxyradical-dependent photoemission during endothelium-granulocyte interaction in microvascular beds treated with platelet-activating factor. In: Journal of Biochemistry. 1989 ; Vol. 106, No. 2. pp. 355-360.
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