In Vivo visualization of subtle, transient, and local activity of astrocytes using an ultrasensitive Ca2+ indicator

Kazunori Kanemaru, Hiroshi Sekiya, Ming Xu, Kaname Satoh, Nami Kitajima, Keitaro Yoshida, Yohei Okubo, Takuya Sasaki, Satoru Moritoh, Hidetoshi Hasuwa, Masaru Mimura, Kazuki Horikawa, Ko Matsui, Takeharu Nagai, Masamitsu Iino, Kenji Tanaka

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Astrocytes generate local calcium (Ca2+) signals that are thought to regulate their functions. Visualization of these signals in the intact brain requires an imaging method with high spatiotemporal resolution. Here, we describe such a method using transgenic mice expressing the ultrasensitive ratiometric Ca2+ indicator yellow Cameleon-Nano 50 (YC-Nano50) inastrocytes. In these mice, we detected a unique pattern of Ca2+ signals. These occur spontaneously, predominantly in astrocytic fine processes, but not the cell body. Upon sensory stimulation, astrocytes initially responded with Ca2+ signals at fine processes, which then propagated to the cell body. These observations suggest that astrocytic fine processes function as a high-sensitivity detector of neuronal activities. Thus, the method provides a useful tool for studying the activity of astrocytes in brain physiology and pathology.

Original languageEnglish
Pages (from-to)311-318
Number of pages8
JournalCell Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2014 Jul 10

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Astrocytes
Visualization
Brain
Cells
Physiology
Pathology
Neuroimaging
Transgenic Mice
Calcium
Detectors
Imaging techniques
Cell Body
yellow cameleon

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

In Vivo visualization of subtle, transient, and local activity of astrocytes using an ultrasensitive Ca2+ indicator. / Kanemaru, Kazunori; Sekiya, Hiroshi; Xu, Ming; Satoh, Kaname; Kitajima, Nami; Yoshida, Keitaro; Okubo, Yohei; Sasaki, Takuya; Moritoh, Satoru; Hasuwa, Hidetoshi; Mimura, Masaru; Horikawa, Kazuki; Matsui, Ko; Nagai, Takeharu; Iino, Masamitsu; Tanaka, Kenji.

In: Cell Reports, Vol. 8, No. 1, 10.07.2014, p. 311-318.

Research output: Contribution to journalArticle

Kanemaru, K, Sekiya, H, Xu, M, Satoh, K, Kitajima, N, Yoshida, K, Okubo, Y, Sasaki, T, Moritoh, S, Hasuwa, H, Mimura, M, Horikawa, K, Matsui, K, Nagai, T, Iino, M & Tanaka, K 2014, 'In Vivo visualization of subtle, transient, and local activity of astrocytes using an ultrasensitive Ca2+ indicator', Cell Reports, vol. 8, no. 1, pp. 311-318. https://doi.org/10.1016/j.celrep.2014.05.056
Kanemaru, Kazunori ; Sekiya, Hiroshi ; Xu, Ming ; Satoh, Kaname ; Kitajima, Nami ; Yoshida, Keitaro ; Okubo, Yohei ; Sasaki, Takuya ; Moritoh, Satoru ; Hasuwa, Hidetoshi ; Mimura, Masaru ; Horikawa, Kazuki ; Matsui, Ko ; Nagai, Takeharu ; Iino, Masamitsu ; Tanaka, Kenji. / In Vivo visualization of subtle, transient, and local activity of astrocytes using an ultrasensitive Ca2+ indicator. In: Cell Reports. 2014 ; Vol. 8, No. 1. pp. 311-318.
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