In Vivo visualization of subtle, transient, and local activity of astrocytes using an ultrasensitive Ca2+ indicator

Kazunori Kanemaru, Hiroshi Sekiya, Ming Xu, Kaname Satoh, Nami Kitajima, Keitaro Yoshida, Yohei Okubo, Takuya Sasaki, Satoru Moritoh, Hidetoshi Hasuwa, Masaru Mimura, Kazuki Horikawa, Ko Matsui, Takeharu Nagai, Masamitsu Iino, Kenji F. Tanaka

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87 Citations (Scopus)

Abstract

Astrocytes generate local calcium (Ca2+) signals that are thought to regulate their functions. Visualization of these signals in the intact brain requires an imaging method with high spatiotemporal resolution. Here, we describe such a method using transgenic mice expressing the ultrasensitive ratiometric Ca2+ indicator yellow Cameleon-Nano 50 (YC-Nano50) inastrocytes. In these mice, we detected a unique pattern of Ca2+ signals. These occur spontaneously, predominantly in astrocytic fine processes, but not the cell body. Upon sensory stimulation, astrocytes initially responded with Ca2+ signals at fine processes, which then propagated to the cell body. These observations suggest that astrocytic fine processes function as a high-sensitivity detector of neuronal activities. Thus, the method provides a useful tool for studying the activity of astrocytes in brain physiology and pathology.

Original languageEnglish
Pages (from-to)311-318
Number of pages8
JournalCell Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2014 Jul 10

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Kanemaru, K., Sekiya, H., Xu, M., Satoh, K., Kitajima, N., Yoshida, K., Okubo, Y., Sasaki, T., Moritoh, S., Hasuwa, H., Mimura, M., Horikawa, K., Matsui, K., Nagai, T., Iino, M., & Tanaka, K. F. (2014). In Vivo visualization of subtle, transient, and local activity of astrocytes using an ultrasensitive Ca2+ indicator. Cell Reports, 8(1), 311-318. https://doi.org/10.1016/j.celrep.2014.05.056