Inactivation of tissue inhibitor of metalloproteinases by neutrophil elastase and other serine proteinases

Yasunod Okada, Shoji Watanabe, Isao Nakanishi, Jun ichi Kishi, Taro Hayakawa, Wieslaw Watorek, James Travis, Hideaki Nagase

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Tissue inhibitor of metalloproteinases (TIMP) from cultured bovine dental pulp inhibits human rheumatoid synovial matrix metalloproteinase 3 (MMP-3) with a stoichiometry of 1:1 on a molar basis. Among the serine proteinases examined, human neutrophil elastase, trypsin and α-chymotrypsin destroyed the inhibitory activity of TIMP against MMP-3 by degrading the inhibitor molecule into small fragments. In contrast, the inhibitory activity of TIMP was not significantly reduced by the actions of cathepsin G, pancreatic elastase and plasmin. These data indicate that neutrophils which infiltrate tissues in various inflammatory conditions may play an important role in regulating TIMP activity in vivo through the action of neutrophil elastase.

Original languageEnglish
Pages (from-to)157-160
Number of pages4
JournalFEBS Letters
Volume229
Issue number1
DOIs
Publication statusPublished - 1988 Feb 29

Fingerprint

Matrix Metalloproteinase 12
Tissue Inhibitor of Metalloproteinases
Leukocyte Elastase
Serine Proteases
Matrix Metalloproteinase 3
Cathepsin G
Dental Pulp
Pancreatic Elastase
Fibrinolysin
Stoichiometry
Pulp
Neutrophils
Tissue
Molecules

Keywords

  • Extracellular matrix
  • Metalloproteinase
  • Neutrophil elastase
  • Tissue inhibitor of metalloproteinases

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Okada, Y., Watanabe, S., Nakanishi, I., Kishi, J. I., Hayakawa, T., Watorek, W., ... Nagase, H. (1988). Inactivation of tissue inhibitor of metalloproteinases by neutrophil elastase and other serine proteinases. FEBS Letters, 229(1), 157-160. https://doi.org/10.1016/0014-5793(88)80817-2

Inactivation of tissue inhibitor of metalloproteinases by neutrophil elastase and other serine proteinases. / Okada, Yasunod; Watanabe, Shoji; Nakanishi, Isao; Kishi, Jun ichi; Hayakawa, Taro; Watorek, Wieslaw; Travis, James; Nagase, Hideaki.

In: FEBS Letters, Vol. 229, No. 1, 29.02.1988, p. 157-160.

Research output: Contribution to journalArticle

Okada, Y, Watanabe, S, Nakanishi, I, Kishi, JI, Hayakawa, T, Watorek, W, Travis, J & Nagase, H 1988, 'Inactivation of tissue inhibitor of metalloproteinases by neutrophil elastase and other serine proteinases', FEBS Letters, vol. 229, no. 1, pp. 157-160. https://doi.org/10.1016/0014-5793(88)80817-2
Okada, Yasunod ; Watanabe, Shoji ; Nakanishi, Isao ; Kishi, Jun ichi ; Hayakawa, Taro ; Watorek, Wieslaw ; Travis, James ; Nagase, Hideaki. / Inactivation of tissue inhibitor of metalloproteinases by neutrophil elastase and other serine proteinases. In: FEBS Letters. 1988 ; Vol. 229, No. 1. pp. 157-160.
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