Increase in synthesis and stability of σ32 on treatment with inhibitors of DNA gyrase in Escherichia coli

T. Mizushima, Y. Ohtsuka, H. Mori, T. Miki, K. Sekimizu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We report here that in Escherichia coli, the anti-bacterial agent nalidixic acid induces transient stabilization and increased synthesis of σ32, accompanied by the induction of heat shock proteins (Dnak and GroEL proteins). The induction of heat shock proteins, increased synthesis of σ32,and stabilization of σ32 observed on treatment of wild-type cells with nalidixic acid were not observed in a nalA26 mutant, a strain that is resistant to nalidixic acid as the result of a mutation in the gyrA gene. Not only oxolinic acid, but also novobiocin, whose targets are the A and B subunits of DNA gyrase, respectively, also induced stabilization and increased synthesis of σ32. Thus, inhibition of the activity of DNA gyrase may cause stabilization and increased synthesis of σ32, resulting in turn in induction of heat shock proteins.

Original languageEnglish
Pages (from-to)297-302
Number of pages6
JournalMolecular and General Genetics
Volume253
Issue number3
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

DNA Gyrase
Topoisomerase II Inhibitors
Nalidixic Acid
Heat-Shock Proteins
Escherichia coli
Oxolinic Acid
Novobiocin
Chaperonin 60
Anti-Bacterial Agents
Mutation
Genes

Keywords

  • σ
  • Heat shock response
  • Inhibitors of DNA gyrase

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Increase in synthesis and stability of σ32 on treatment with inhibitors of DNA gyrase in Escherichia coli. / Mizushima, T.; Ohtsuka, Y.; Mori, H.; Miki, T.; Sekimizu, K.

In: Molecular and General Genetics, Vol. 253, No. 3, 1996, p. 297-302.

Research output: Contribution to journalArticle

Mizushima, T. ; Ohtsuka, Y. ; Mori, H. ; Miki, T. ; Sekimizu, K. / Increase in synthesis and stability of σ32 on treatment with inhibitors of DNA gyrase in Escherichia coli. In: Molecular and General Genetics. 1996 ; Vol. 253, No. 3. pp. 297-302.
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