Increase in the Capability of Interleukin 2 Gene-Transduced Renal Cell Carcinoma Cells to Induce Cytotoxic Lymphocytes

Kyogo Itoh, Yasuiki Umezu, Tatsuo Morita, Dai Seito, Lazel B. Augustus, Hideyuki Saya, Kyogo Itoh, Masanobu Nakao, Motoko Sakata, Jiro Miyajima, Kazuhiro Masuoka, Hiroshi Matsui

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Abstract

The immunogenicity of human cancer cells transfected with interleukin 2 (IL-2) gene, a potent vaccine candidate, has not yet been fully investigated. Human renal cell carcinoma (RCC) cells transduced with human IL-2 gene (RCC-IL-2) were investigated in vitro for the capability to induce lymphokine-activated killer (LAK) or cytotoxic T lymphocyte (CTL) activity in peripheral blood mononuclear cells (PBMC) or tumor-infiltrating lymphocyte (TIL). The RCC-IL-2 cells stimulated PBMC to demonstrate LAK activity, and also stimulated autologous TILs to proliferate and exhibit cytotoxicity relatively restricted to autologous tumor cells. In contrast, both parental RCC and RCC transduced with neomycin gene alone failed to induce these activities. These results indicate that RCC-IL-2 cells are more potent than the other RCC cells with regard to inducing cytotoxic lymphocytes against autologous tumor cells.

Original languageEnglish
Pages (from-to)53-63
Number of pages11
JournalThe Kurume Medical Journal
Volume41
Issue number2
DOIs
Publication statusPublished - 1994 Jan 1
Externally publishedYes

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Keywords

  • cytotoxic T lymphocytes
  • gene transduction
  • immunogenicity
  • interleukin 2 gene
  • renal cell carcinoma

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Itoh, K., Umezu, Y., Morita, T., Seito, D., Augustus, L. B., Saya, H., Itoh, K., Nakao, M., Sakata, M., Miyajima, J., Masuoka, K., & Matsui, H. (1994). Increase in the Capability of Interleukin 2 Gene-Transduced Renal Cell Carcinoma Cells to Induce Cytotoxic Lymphocytes. The Kurume Medical Journal, 41(2), 53-63. https://doi.org/10.2739/kurumemedj.41.53