Increase of smooth muscle cell migration and of intimal hyperplasia in mice lacking the α/β hydrolase domain containing 2 gene

Keishi Miyata, Yuichi Oike, Takayuki Hoshii, Hiromitsu Maekawa, Hisao Ogawa, Toshio Suda, Kimi Araki, Ken Ichi Yamamura

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Multiple steps, including the migration of vascular smooth muscle cells (SMCs), are involved in the pathogenesis of atherosclerosis. To discover genes which are involved in these steps, we screened mutant mouse lines established by the exchangeable gene trap method utilizing X-gal staining during their embryonic development. One of these lines showed strong reporter gene expression in the vitelline vessels of yolk sacs at embryonic day (E) 12.5. The trap vector was inserted into the fifth intron of α/β hydrolase domain containing 2 (Abhd2) gene which was shown to be expressed in vascular and non-vascular SMCs of adult mice. Although homozygous mutant mice were apparently normal, enhanced SMC migration in the explants SMCs culture and marked intimal hyperplasia after cuff placement were observed in homozygous mice in comparison with wild-type mice. Our results show that Abhd2 is involved in SMC migration and neointimal thickening on vascular SMCs.

Original languageEnglish
Pages (from-to)296-304
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume329
Issue number1
DOIs
Publication statusPublished - 2005 Apr 1

Keywords

  • Abhd2
  • Alveolar type II cell
  • Atherosclerosis
  • Cuff placement model
  • Gene trap
  • Hepatocyte
  • Migration
  • Neointimal hyperplasia
  • Smooth muscle cell
  • α/β hydrolase protein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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