Increased activation of CCAAT/enhancer binding protein-β correlates with the invasiveness of renal cell carcinoma

Mototsugu Oya, Akio Horiguchi, Ryuichi Mizuno, Ken Marumo, Masaru Murai

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Positive inflammatory reactions in an aggressive phenotype are typical features of renal cell carcinoma (RCC). Although a high blood level of inflammatory cytokines, such as interleukin-6, interleukin-8, and tumor necrosis factor-α, has been observed in these patients, the mechanisms underlying this clinical phenomenon remain to be elucidated. CCAAT/enhancer binding protein (C/EBP) family are transcription factors which play a role in cell differentiation and inflammatory reactions. Among these, C/EBP-β induces a variety of cytokines and thus may play a role in the pathogenesis of RCC. We studied the activation of C/EBP-β determined by electrophoretic mobility shift assay in nine RCC cell lines and 44 tissue samples. Six cell lines showed an activation of C/EBP-β, whereas three cell lines did not, and two of these three had no expression at all of C/EBP-β protein. Of 44 tissue samples, 12 (27.3%) showed a >200% increase in the activity compared with the corresponding normal kidney tissues. Locally advanced cases had a significantly higher rate of increased C/EBP-β activity (5 of 8 = 62.5% in advanced cases versus 7 of 36 = 19.4% in localized cases). Especially, all four cases with renal vein invasion had an increased C/EBP-β activity. These data suggest that the increased activation of C/EBP-β may contribute to promote tumor invasiveness and render a malignant phenotype of RCC, although it needs to be validated in a larger series.

Original languageEnglish
Pages (from-to)1021-1027
Number of pages7
JournalClinical Cancer Research
Volume9
Issue number3
Publication statusPublished - 2003 Mar 1

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CCAAT-Enhancer-Binding Proteins
Renal Cell Carcinoma
Cell Line
Cytokines
Phenotype
Renal Veins
Electrophoretic Mobility Shift Assay
Interleukin-8
Cell Differentiation
Interleukin-6
Transcription Factors
Tumor Necrosis Factor-alpha
Kidney

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Increased activation of CCAAT/enhancer binding protein-β correlates with the invasiveness of renal cell carcinoma. / Oya, Mototsugu; Horiguchi, Akio; Mizuno, Ryuichi; Marumo, Ken; Murai, Masaru.

In: Clinical Cancer Research, Vol. 9, No. 3, 01.03.2003, p. 1021-1027.

Research output: Contribution to journalArticle

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