Increased basigin in bleomycin-induced lung injury

Tomoko Betsuyaku, Kenji Kadomatsu, Gail L. Griffin, Takashi Muramatsu, Robert M. Senior

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Basigin is expressed in many tissues during development, including lung. It is also found on tumor cells and in wounds where it is thought to stimulate adjacent fibroblasts to produce matrix metalloproteinases. To investigate whether basigin might be expressed in fibro-inflammatory lung processes, we generated bleomycin-induced lung injury in mice. At 14 d after intratracheal bleomycin, we found basigin prominently in areas of fibrosis, alveolar macrophages, and bronchiolar epithelium, whereas it was only weakly present in bronchiolar epithelium in untreated mice. Western blots of radioimmunoprecipitation assay RIPA-insoluble fractions of bleomycin-treated lungs showed increased basigin compared with RIPA-insoluble fractions of lung from untreated mice. By quantitative reverse transcriptase-polymerase chain reaction, lung basigin mRNA was significantly increased 14 d after bleomycin, and by in situ hybridization, basigin mRNA was prominent in bronchiolar epithelium. Western blots of bronchoalveolar lavage fluid (BALF) showed various forms of basigin after bleomycin that were not present in BALF from untreated lung. These results demonstrate that bleomycin-induced lung injury is associated with increased basigin expression in bronchiolar epithelium, deposition of basigin in fibrotic sites, and increased basigin in BALF. Accordingly, basigin may play a role in diffuse alveolar injury.

Original languageEnglish
Pages (from-to)600-606
Number of pages7
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume28
Issue number5
DOIs
Publication statusPublished - 2003 May 1
Externally publishedYes

Fingerprint

CD147 Antigens
Lung Injury
Bleomycin
Lung
Bronchoalveolar Lavage Fluid
Epithelium
Fluids
indium-bleomycin
Radioimmunoprecipitation Assay
Western Blotting
Messenger RNA
Polymerase chain reaction
RNA-Directed DNA Polymerase
Wounds and Injuries
Alveolar Macrophages
Fibroblasts
Reverse Transcriptase Polymerase Chain Reaction
Matrix Metalloproteinases
In Situ Hybridization

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Betsuyaku, T., Kadomatsu, K., Griffin, G. L., Muramatsu, T., & Senior, R. M. (2003). Increased basigin in bleomycin-induced lung injury. American Journal of Respiratory Cell and Molecular Biology, 28(5), 600-606. https://doi.org/10.1165/rcmb.2002-0059OC

Increased basigin in bleomycin-induced lung injury. / Betsuyaku, Tomoko; Kadomatsu, Kenji; Griffin, Gail L.; Muramatsu, Takashi; Senior, Robert M.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 28, No. 5, 01.05.2003, p. 600-606.

Research output: Contribution to journalArticle

Betsuyaku, T, Kadomatsu, K, Griffin, GL, Muramatsu, T & Senior, RM 2003, 'Increased basigin in bleomycin-induced lung injury', American Journal of Respiratory Cell and Molecular Biology, vol. 28, no. 5, pp. 600-606. https://doi.org/10.1165/rcmb.2002-0059OC
Betsuyaku, Tomoko ; Kadomatsu, Kenji ; Griffin, Gail L. ; Muramatsu, Takashi ; Senior, Robert M. / Increased basigin in bleomycin-induced lung injury. In: American Journal of Respiratory Cell and Molecular Biology. 2003 ; Vol. 28, No. 5. pp. 600-606.
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