Increased DNA methyltransferase expression is associated with an early stage of human hepatocarcinogenesis

Lin Sun, Ai Min Hui, Yae Kanai, Michiie Sakamoto, Setsuo Hirohashi

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The present study was designed to determine whether changes in DNA methyltransferase (DNA MTase) expression are involved in hepatocarcinogenesis. We examined DNA MTase expression in normal liver tissue (with no remarkable histological findings), liver tissue showing chronic hepatitis or cirrhosis, which are generally thought to be precancerous conditions, and hepatocellular carcinomas (HCCs) using the reverse-transcriptase polymerase chain reaction assay. DNA MTase mRNA levels were significantly higher in liver tissue showing chronic hepatitis and cirrhosis (DNA MTase mRNA/β-actin mRNA ratio = 0.30 ± 0.22, n = 24, P < 0.01) than in normal liver tissue either from patients with liver metastatic lesions of colonic cancer (0.14 ± 0.05, n = 6) or from patients with HCCs (0.16 ± 0.07, n = 3). DNA MTase mRNA levels were even higher in HCC tissue (0.34 ± 0.18, n = 29). These results suggest that increased DNA MTase expression may be an early event during hepatocarcinogenesis. DNA MTase is a potential target for HCC preventive therapy.

Original languageEnglish
Pages (from-to)1165-1170
Number of pages6
JournalJapanese Journal of Cancer Research
Issue number12
Publication statusPublished - 1997 Dec
Externally publishedYes



  • Chronic hepatitis
  • Cirrhosis
  • DNA methyltransferase
  • Hepatocellular carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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