Increased DNA methyltransferase expression is associated with an early stage of human hepatocarcinogenesis

Lin Sun; Ai Min Hui; Yae Kanai; Michiie Sakamoto; Setsuo Hirohashi

doi:10.1111/j.1349-7006.1997.tb00345.x

Increased DNA methyltransferase expression is associated with an early stage of human hepatocarcinogenesis

Lin Sun, Ai Min Hui, Yae Kanai, Michiie Sakamoto, Setsuo Hirohashi

Research output: Contribution to journal › Article › peer-review

86 Citations (Scopus)

Abstract

The present study was designed to determine whether changes in DNA methyltransferase (DNA MTase) expression are involved in hepatocarcinogenesis. We examined DNA MTase expression in normal liver tissue (with no remarkable histological findings), liver tissue showing chronic hepatitis or cirrhosis, which are generally thought to be precancerous conditions, and hepatocellular carcinomas (HCCs) using the reverse-transcriptase polymerase chain reaction assay. DNA MTase mRNA levels were significantly higher in liver tissue showing chronic hepatitis and cirrhosis (DNA MTase mRNA/β-actin mRNA ratio = 0.30 ± 0.22, n = 24, P < 0.01) than in normal liver tissue either from patients with liver metastatic lesions of colonic cancer (0.14 ± 0.05, n = 6) or from patients with HCCs (0.16 ± 0.07, n = 3). DNA MTase mRNA levels were even higher in HCC tissue (0.34 ± 0.18, n = 29). These results suggest that increased DNA MTase expression may be an early event during hepatocarcinogenesis. DNA MTase is a potential target for HCC preventive therapy.

Original language	English
Pages (from-to)	1165-1170
Number of pages	6
Journal	Japanese Journal of Cancer Research
Volume	88
Issue number	12
DOIs	https://doi.org/10.1111/j.1349-7006.1997.tb00345.x
Publication status	Published - 1997 Dec
Externally published	Yes

Keywords

Chronic hepatitis
Cirrhosis
DNA methyltransferase
Hepatocellular carcinoma

ASJC Scopus subject areas

Oncology
Cancer Research

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title = "Increased DNA methyltransferase expression is associated with an early stage of human hepatocarcinogenesis",

abstract = "The present study was designed to determine whether changes in DNA methyltransferase (DNA MTase) expression are involved in hepatocarcinogenesis. We examined DNA MTase expression in normal liver tissue (with no remarkable histological findings), liver tissue showing chronic hepatitis or cirrhosis, which are generally thought to be precancerous conditions, and hepatocellular carcinomas (HCCs) using the reverse-transcriptase polymerase chain reaction assay. DNA MTase mRNA levels were significantly higher in liver tissue showing chronic hepatitis and cirrhosis (DNA MTase mRNA/β-actin mRNA ratio = 0.30 ± 0.22, n = 24, P < 0.01) than in normal liver tissue either from patients with liver metastatic lesions of colonic cancer (0.14 ± 0.05, n = 6) or from patients with HCCs (0.16 ± 0.07, n = 3). DNA MTase mRNA levels were even higher in HCC tissue (0.34 ± 0.18, n = 29). These results suggest that increased DNA MTase expression may be an early event during hepatocarcinogenesis. DNA MTase is a potential target for HCC preventive therapy.",

keywords = "Chronic hepatitis, Cirrhosis, DNA methyltransferase, Hepatocellular carcinoma",

author = "Lin Sun and Hui, {Ai Min} and Yae Kanai and Michiie Sakamoto and Setsuo Hirohashi",

year = "1997",

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doi = "10.1111/j.1349-7006.1997.tb00345.x",

language = "English",

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AU - Hui, Ai Min

AU - Kanai, Yae

AU - Sakamoto, Michiie

AU - Hirohashi, Setsuo

PY - 1997/12

Y1 - 1997/12

N2 - The present study was designed to determine whether changes in DNA methyltransferase (DNA MTase) expression are involved in hepatocarcinogenesis. We examined DNA MTase expression in normal liver tissue (with no remarkable histological findings), liver tissue showing chronic hepatitis or cirrhosis, which are generally thought to be precancerous conditions, and hepatocellular carcinomas (HCCs) using the reverse-transcriptase polymerase chain reaction assay. DNA MTase mRNA levels were significantly higher in liver tissue showing chronic hepatitis and cirrhosis (DNA MTase mRNA/β-actin mRNA ratio = 0.30 ± 0.22, n = 24, P < 0.01) than in normal liver tissue either from patients with liver metastatic lesions of colonic cancer (0.14 ± 0.05, n = 6) or from patients with HCCs (0.16 ± 0.07, n = 3). DNA MTase mRNA levels were even higher in HCC tissue (0.34 ± 0.18, n = 29). These results suggest that increased DNA MTase expression may be an early event during hepatocarcinogenesis. DNA MTase is a potential target for HCC preventive therapy.

AB - The present study was designed to determine whether changes in DNA methyltransferase (DNA MTase) expression are involved in hepatocarcinogenesis. We examined DNA MTase expression in normal liver tissue (with no remarkable histological findings), liver tissue showing chronic hepatitis or cirrhosis, which are generally thought to be precancerous conditions, and hepatocellular carcinomas (HCCs) using the reverse-transcriptase polymerase chain reaction assay. DNA MTase mRNA levels were significantly higher in liver tissue showing chronic hepatitis and cirrhosis (DNA MTase mRNA/β-actin mRNA ratio = 0.30 ± 0.22, n = 24, P < 0.01) than in normal liver tissue either from patients with liver metastatic lesions of colonic cancer (0.14 ± 0.05, n = 6) or from patients with HCCs (0.16 ± 0.07, n = 3). DNA MTase mRNA levels were even higher in HCC tissue (0.34 ± 0.18, n = 29). These results suggest that increased DNA MTase expression may be an early event during hepatocarcinogenesis. DNA MTase is a potential target for HCC preventive therapy.

KW - Chronic hepatitis

KW - Cirrhosis

KW - DNA methyltransferase

KW - Hepatocellular carcinoma

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