Increased drug resistance of cultured human cancer cell lines in three- dimensional cellular growth assay using collagen gel matrix

Toshiharu Furukawa, T. Kubota, M. Watanabe, S. Kase, Y. Saikawa, H. Nishibori, T. H. Kuo -, S. Kodaira, K. Ishibiki, M. Kitajima

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have conducted a three-dimensional cellular growth assay using collagen gel matrix with an endpoint of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- 2H tetrazolium bromide (MTT) assay, on four human gastric and colonic cancer cell lines. Three-dimensionally growing cells in collagen gel matrix were 2- to 180-fold more resistant to mitomycin C, doxorubicin, 5-fluorouracil, and cisplatin than those in monolayer culture, and these resistances were increased further when the cells increased their three-dimensionality. Furthermore, the influence of fibroblasts in the collagen gel matrix on the chemosensitivity of cancer cells was less than that in monolayer culture. Since this new assay using collagen gel matrix with an endpoint of the MTT assay was able to detect the increase of drug resistance of human cancer cell lines by three-dimensional cellular growth using a simple and convenient procedure, it was considered to be more useful than conventional monolayer cultures for evaluating the chemosensitivity of cancer cells.

Original languageEnglish
Pages (from-to)86-92
Number of pages7
JournalJournal of Surgical Oncology
Volume49
Issue number2
Publication statusPublished - 1992

Fingerprint

Drug Resistance
Collagen
Gels
Cell Line
Growth
Neoplasms
Endpoint Determination
Mitomycin
Fluorouracil
Doxorubicin
Colonic Neoplasms
Cisplatin
Stomach Neoplasms
Fibroblasts

Keywords

  • chemosensitivity test
  • fibroblasts
  • MTT assay

ASJC Scopus subject areas

  • Oncology
  • Surgery

Cite this

Increased drug resistance of cultured human cancer cell lines in three- dimensional cellular growth assay using collagen gel matrix. / Furukawa, Toshiharu; Kubota, T.; Watanabe, M.; Kase, S.; Saikawa, Y.; Nishibori, H.; Kuo -, T. H.; Kodaira, S.; Ishibiki, K.; Kitajima, M.

In: Journal of Surgical Oncology, Vol. 49, No. 2, 1992, p. 86-92.

Research output: Contribution to journalArticle

Furukawa, T, Kubota, T, Watanabe, M, Kase, S, Saikawa, Y, Nishibori, H, Kuo -, TH, Kodaira, S, Ishibiki, K & Kitajima, M 1992, 'Increased drug resistance of cultured human cancer cell lines in three- dimensional cellular growth assay using collagen gel matrix', Journal of Surgical Oncology, vol. 49, no. 2, pp. 86-92.
Furukawa, Toshiharu ; Kubota, T. ; Watanabe, M. ; Kase, S. ; Saikawa, Y. ; Nishibori, H. ; Kuo -, T. H. ; Kodaira, S. ; Ishibiki, K. ; Kitajima, M. / Increased drug resistance of cultured human cancer cell lines in three- dimensional cellular growth assay using collagen gel matrix. In: Journal of Surgical Oncology. 1992 ; Vol. 49, No. 2. pp. 86-92.
@article{cc63e4183f7f4525a96dc11249dcc6db,
title = "Increased drug resistance of cultured human cancer cell lines in three- dimensional cellular growth assay using collagen gel matrix",
abstract = "We have conducted a three-dimensional cellular growth assay using collagen gel matrix with an endpoint of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- 2H tetrazolium bromide (MTT) assay, on four human gastric and colonic cancer cell lines. Three-dimensionally growing cells in collagen gel matrix were 2- to 180-fold more resistant to mitomycin C, doxorubicin, 5-fluorouracil, and cisplatin than those in monolayer culture, and these resistances were increased further when the cells increased their three-dimensionality. Furthermore, the influence of fibroblasts in the collagen gel matrix on the chemosensitivity of cancer cells was less than that in monolayer culture. Since this new assay using collagen gel matrix with an endpoint of the MTT assay was able to detect the increase of drug resistance of human cancer cell lines by three-dimensional cellular growth using a simple and convenient procedure, it was considered to be more useful than conventional monolayer cultures for evaluating the chemosensitivity of cancer cells.",
keywords = "chemosensitivity test, fibroblasts, MTT assay",
author = "Toshiharu Furukawa and T. Kubota and M. Watanabe and S. Kase and Y. Saikawa and H. Nishibori and {Kuo -}, {T. H.} and S. Kodaira and K. Ishibiki and M. Kitajima",
year = "1992",
language = "English",
volume = "49",
pages = "86--92",
journal = "Journal of Surgical Oncology",
issn = "0022-4790",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Increased drug resistance of cultured human cancer cell lines in three- dimensional cellular growth assay using collagen gel matrix

AU - Furukawa, Toshiharu

AU - Kubota, T.

AU - Watanabe, M.

AU - Kase, S.

AU - Saikawa, Y.

AU - Nishibori, H.

AU - Kuo -, T. H.

AU - Kodaira, S.

AU - Ishibiki, K.

AU - Kitajima, M.

PY - 1992

Y1 - 1992

N2 - We have conducted a three-dimensional cellular growth assay using collagen gel matrix with an endpoint of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- 2H tetrazolium bromide (MTT) assay, on four human gastric and colonic cancer cell lines. Three-dimensionally growing cells in collagen gel matrix were 2- to 180-fold more resistant to mitomycin C, doxorubicin, 5-fluorouracil, and cisplatin than those in monolayer culture, and these resistances were increased further when the cells increased their three-dimensionality. Furthermore, the influence of fibroblasts in the collagen gel matrix on the chemosensitivity of cancer cells was less than that in monolayer culture. Since this new assay using collagen gel matrix with an endpoint of the MTT assay was able to detect the increase of drug resistance of human cancer cell lines by three-dimensional cellular growth using a simple and convenient procedure, it was considered to be more useful than conventional monolayer cultures for evaluating the chemosensitivity of cancer cells.

AB - We have conducted a three-dimensional cellular growth assay using collagen gel matrix with an endpoint of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- 2H tetrazolium bromide (MTT) assay, on four human gastric and colonic cancer cell lines. Three-dimensionally growing cells in collagen gel matrix were 2- to 180-fold more resistant to mitomycin C, doxorubicin, 5-fluorouracil, and cisplatin than those in monolayer culture, and these resistances were increased further when the cells increased their three-dimensionality. Furthermore, the influence of fibroblasts in the collagen gel matrix on the chemosensitivity of cancer cells was less than that in monolayer culture. Since this new assay using collagen gel matrix with an endpoint of the MTT assay was able to detect the increase of drug resistance of human cancer cell lines by three-dimensional cellular growth using a simple and convenient procedure, it was considered to be more useful than conventional monolayer cultures for evaluating the chemosensitivity of cancer cells.

KW - chemosensitivity test

KW - fibroblasts

KW - MTT assay

UR - http://www.scopus.com/inward/record.url?scp=0026602561&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026602561&partnerID=8YFLogxK

M3 - Article

C2 - 1738241

AN - SCOPUS:0026602561

VL - 49

SP - 86

EP - 92

JO - Journal of Surgical Oncology

JF - Journal of Surgical Oncology

SN - 0022-4790

IS - 2

ER -