Increased expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) and lymphocyte recruitment in murine gastritis induced by Helicobacter pylori

K. Hatanaka, R. Hokari, K. Matsuzaki, S. Kato, A. Kawaguchi, S. Nagao, Hidekazu Suzuki, K. Miyazaki, E. Sekizuka, H. Nagata, H. Ishii, S. Miura

Research output: Contribution to journalArticle

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Abstract

Although T cell involvement in Helicobactor pylori-induced gastritis is known, mechanism about T cell recruitment is not understood. In this study we examined how mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is involved in lymphocyte recruitment in murine chronic gastritis induced by H. pylori. C57 BL/6 mice were infected with Sydney strain (SS1). Six months after infection, the stomach was removed. The expression of adhesion molecules, MAdCAM-1, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), and the cell surface antigens CD4, CD8, CD45R/B220 or β7-integrin were determined by immunohistochemistry. A significant increase in CD4 lymphocytes was observed in the body portion of stomach in SS1-infected mice and most of these CD4 cells express β7-integrin, a known counter ligand for MAdCAM-1 molecule. Strong MAdCAM-1 expression was observed adjacent to these cells in the lamina propria as well as in the submucosa of SS1-infected stomach. Quantitative analysis showed that the area of MAdCAM-1 expression well correlated with the infiltration of β7-integrin positive lymphocytes. On the other hand, expression of ICAM-1 or VCAM-1 in the lamina propria was few even in the SS1-infected stomach. Increased expression of MAdCAM-1 was well correlated to the location of lymphocytes, which express CD4 and β7-integrin. These results suggest the possibility that MAdCAM-1 may be largely involved in the lymphocyte recruitment in the gastritis mucosa with H. pylori.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalClinical and Experimental Immunology
Volume130
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

Cell Adhesion Molecules
Gastritis
Helicobacter pylori
Lymphocytes
Integrins
Stomach
Mucous Membrane
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
T-Lymphocytes
Pylorus
Surface Antigens
Immunohistochemistry
Ligands
Infection

Keywords

  • β7-integrin
  • Adhesion molecules
  • Helicobactor pylori gastritis
  • Lymphocyte migration mucosal addressin cell adhesion molecule-1 (MAdCAM-1)

ASJC Scopus subject areas

  • Immunology

Cite this

Increased expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) and lymphocyte recruitment in murine gastritis induced by Helicobacter pylori. / Hatanaka, K.; Hokari, R.; Matsuzaki, K.; Kato, S.; Kawaguchi, A.; Nagao, S.; Suzuki, Hidekazu; Miyazaki, K.; Sekizuka, E.; Nagata, H.; Ishii, H.; Miura, S.

In: Clinical and Experimental Immunology, Vol. 130, No. 2, 2002, p. 183-189.

Research output: Contribution to journalArticle

Hatanaka, K, Hokari, R, Matsuzaki, K, Kato, S, Kawaguchi, A, Nagao, S, Suzuki, H, Miyazaki, K, Sekizuka, E, Nagata, H, Ishii, H & Miura, S 2002, 'Increased expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) and lymphocyte recruitment in murine gastritis induced by Helicobacter pylori', Clinical and Experimental Immunology, vol. 130, no. 2, pp. 183-189. https://doi.org/10.1046/j.1365-2249.2002.01984.x
Hatanaka, K. ; Hokari, R. ; Matsuzaki, K. ; Kato, S. ; Kawaguchi, A. ; Nagao, S. ; Suzuki, Hidekazu ; Miyazaki, K. ; Sekizuka, E. ; Nagata, H. ; Ishii, H. ; Miura, S. / Increased expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) and lymphocyte recruitment in murine gastritis induced by Helicobacter pylori. In: Clinical and Experimental Immunology. 2002 ; Vol. 130, No. 2. pp. 183-189.
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AU - Hokari, R.

AU - Matsuzaki, K.

AU - Kato, S.

AU - Kawaguchi, A.

AU - Nagao, S.

AU - Suzuki, Hidekazu

AU - Miyazaki, K.

AU - Sekizuka, E.

AU - Nagata, H.

AU - Ishii, H.

AU - Miura, S.

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AB - Although T cell involvement in Helicobactor pylori-induced gastritis is known, mechanism about T cell recruitment is not understood. In this study we examined how mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is involved in lymphocyte recruitment in murine chronic gastritis induced by H. pylori. C57 BL/6 mice were infected with Sydney strain (SS1). Six months after infection, the stomach was removed. The expression of adhesion molecules, MAdCAM-1, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), and the cell surface antigens CD4, CD8, CD45R/B220 or β7-integrin were determined by immunohistochemistry. A significant increase in CD4 lymphocytes was observed in the body portion of stomach in SS1-infected mice and most of these CD4 cells express β7-integrin, a known counter ligand for MAdCAM-1 molecule. Strong MAdCAM-1 expression was observed adjacent to these cells in the lamina propria as well as in the submucosa of SS1-infected stomach. Quantitative analysis showed that the area of MAdCAM-1 expression well correlated with the infiltration of β7-integrin positive lymphocytes. On the other hand, expression of ICAM-1 or VCAM-1 in the lamina propria was few even in the SS1-infected stomach. Increased expression of MAdCAM-1 was well correlated to the location of lymphocytes, which express CD4 and β7-integrin. These results suggest the possibility that MAdCAM-1 may be largely involved in the lymphocyte recruitment in the gastritis mucosa with H. pylori.

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