Increased expression of the histamine H 4 receptor subtype in hypertrophic differentiation of chondrogenic ATDC5 cells

Katsunori Yamaura, Seri Akiyama, Koichi Ueno

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Histamine has been regarded as an inflammatory mediator of arthritic disorders. We have previously reported that the expression of histamine H 4 receptor (H 4R) mRNA in synovial tissues was significantly higher in patients with osteoarthritis (OA) compared to those with rheumatoid arthritis. Chondrocyte hypertrophy and endochondral ossification are essential processes in pathologic disorders such as osteophyte formation during OA progression. In the present study, we examined the expression of H 4R during differentiation into hypertrophic chondrocytes in the ATDC5 cells, a widely used in vitro model of chondrogenic differentiation. Quantitative reverse transcription polymerase chain reaction showed that the levels of histidine decarboxylase and H 4R mRNA on ATDC5 cells were increased in a time-dependent manner during the culture period. By contrast, the expressions of H1R and H2R were not increased from day 7 onwards. The mRNA expression of the hypertrophic chondrocyte marker type X collagen (COL X) was increased markedly from 14 to 21. Immunocytochemical analysis indicated that H 4R staining was strongly immunoreactive on the plasma membrane of ATDC5 cells. Flow cytometry showed increased expression of H 4R and COL X protein in ATDC5 chondrocytes. Furthermore, the majority of the COL X-positive cells expressed H 4R throughout the culture period. In summary, we showed for the first time that H 4R is expressed in ATDC5 chondrocytes. Moreover, we found that most hypertrophic chondrocytes express H 4R, suggesting that this receptor might be associated with the differentiation of chondrocytes into hypertrophic cells, which are abnormally observed in joint lesions in OA.

Original languageEnglish
Pages (from-to)1054-1060
Number of pages7
JournalJournal of Cellular Biochemistry
Volume113
Issue number3
DOIs
Publication statusPublished - 2012 Mar
Externally publishedYes

Fingerprint

Chondrocytes
Histamine
Messenger RNA
Collagen Type X
Histidine Decarboxylase
Osteoarthritis
Flow cytometry
Polymerase chain reaction
Transcription
Cell membranes
Cell culture
Cells
Tissue
Osteophyte
Pathologic Processes
Osteogenesis
Proteins
Hypertrophy
Reverse Transcription
Arthritis

Keywords

  • ATDC5
  • Histamine H Receptor (H R)
  • Hypertrophic Chondrocyte
  • Osteoarthritis (Oa)
  • Type X Collagen

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Increased expression of the histamine H 4 receptor subtype in hypertrophic differentiation of chondrogenic ATDC5 cells. / Yamaura, Katsunori; Akiyama, Seri; Ueno, Koichi.

In: Journal of Cellular Biochemistry, Vol. 113, No. 3, 03.2012, p. 1054-1060.

Research output: Contribution to journalArticle

@article{7b18876adfee47a1a9b5216e8c9cdc58,
title = "Increased expression of the histamine H 4 receptor subtype in hypertrophic differentiation of chondrogenic ATDC5 cells",
abstract = "Histamine has been regarded as an inflammatory mediator of arthritic disorders. We have previously reported that the expression of histamine H 4 receptor (H 4R) mRNA in synovial tissues was significantly higher in patients with osteoarthritis (OA) compared to those with rheumatoid arthritis. Chondrocyte hypertrophy and endochondral ossification are essential processes in pathologic disorders such as osteophyte formation during OA progression. In the present study, we examined the expression of H 4R during differentiation into hypertrophic chondrocytes in the ATDC5 cells, a widely used in vitro model of chondrogenic differentiation. Quantitative reverse transcription polymerase chain reaction showed that the levels of histidine decarboxylase and H 4R mRNA on ATDC5 cells were increased in a time-dependent manner during the culture period. By contrast, the expressions of H1R and H2R were not increased from day 7 onwards. The mRNA expression of the hypertrophic chondrocyte marker type X collagen (COL X) was increased markedly from 14 to 21. Immunocytochemical analysis indicated that H 4R staining was strongly immunoreactive on the plasma membrane of ATDC5 cells. Flow cytometry showed increased expression of H 4R and COL X protein in ATDC5 chondrocytes. Furthermore, the majority of the COL X-positive cells expressed H 4R throughout the culture period. In summary, we showed for the first time that H 4R is expressed in ATDC5 chondrocytes. Moreover, we found that most hypertrophic chondrocytes express H 4R, suggesting that this receptor might be associated with the differentiation of chondrocytes into hypertrophic cells, which are abnormally observed in joint lesions in OA.",
keywords = "ATDC5, Histamine H Receptor (H R), Hypertrophic Chondrocyte, Osteoarthritis (Oa), Type X Collagen",
author = "Katsunori Yamaura and Seri Akiyama and Koichi Ueno",
year = "2012",
month = "3",
doi = "10.1002/jcb.23436",
language = "English",
volume = "113",
pages = "1054--1060",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Increased expression of the histamine H 4 receptor subtype in hypertrophic differentiation of chondrogenic ATDC5 cells

AU - Yamaura, Katsunori

AU - Akiyama, Seri

AU - Ueno, Koichi

PY - 2012/3

Y1 - 2012/3

N2 - Histamine has been regarded as an inflammatory mediator of arthritic disorders. We have previously reported that the expression of histamine H 4 receptor (H 4R) mRNA in synovial tissues was significantly higher in patients with osteoarthritis (OA) compared to those with rheumatoid arthritis. Chondrocyte hypertrophy and endochondral ossification are essential processes in pathologic disorders such as osteophyte formation during OA progression. In the present study, we examined the expression of H 4R during differentiation into hypertrophic chondrocytes in the ATDC5 cells, a widely used in vitro model of chondrogenic differentiation. Quantitative reverse transcription polymerase chain reaction showed that the levels of histidine decarboxylase and H 4R mRNA on ATDC5 cells were increased in a time-dependent manner during the culture period. By contrast, the expressions of H1R and H2R were not increased from day 7 onwards. The mRNA expression of the hypertrophic chondrocyte marker type X collagen (COL X) was increased markedly from 14 to 21. Immunocytochemical analysis indicated that H 4R staining was strongly immunoreactive on the plasma membrane of ATDC5 cells. Flow cytometry showed increased expression of H 4R and COL X protein in ATDC5 chondrocytes. Furthermore, the majority of the COL X-positive cells expressed H 4R throughout the culture period. In summary, we showed for the first time that H 4R is expressed in ATDC5 chondrocytes. Moreover, we found that most hypertrophic chondrocytes express H 4R, suggesting that this receptor might be associated with the differentiation of chondrocytes into hypertrophic cells, which are abnormally observed in joint lesions in OA.

AB - Histamine has been regarded as an inflammatory mediator of arthritic disorders. We have previously reported that the expression of histamine H 4 receptor (H 4R) mRNA in synovial tissues was significantly higher in patients with osteoarthritis (OA) compared to those with rheumatoid arthritis. Chondrocyte hypertrophy and endochondral ossification are essential processes in pathologic disorders such as osteophyte formation during OA progression. In the present study, we examined the expression of H 4R during differentiation into hypertrophic chondrocytes in the ATDC5 cells, a widely used in vitro model of chondrogenic differentiation. Quantitative reverse transcription polymerase chain reaction showed that the levels of histidine decarboxylase and H 4R mRNA on ATDC5 cells were increased in a time-dependent manner during the culture period. By contrast, the expressions of H1R and H2R were not increased from day 7 onwards. The mRNA expression of the hypertrophic chondrocyte marker type X collagen (COL X) was increased markedly from 14 to 21. Immunocytochemical analysis indicated that H 4R staining was strongly immunoreactive on the plasma membrane of ATDC5 cells. Flow cytometry showed increased expression of H 4R and COL X protein in ATDC5 chondrocytes. Furthermore, the majority of the COL X-positive cells expressed H 4R throughout the culture period. In summary, we showed for the first time that H 4R is expressed in ATDC5 chondrocytes. Moreover, we found that most hypertrophic chondrocytes express H 4R, suggesting that this receptor might be associated with the differentiation of chondrocytes into hypertrophic cells, which are abnormally observed in joint lesions in OA.

KW - ATDC5

KW - Histamine H Receptor (H R)

KW - Hypertrophic Chondrocyte

KW - Osteoarthritis (Oa)

KW - Type X Collagen

UR - http://www.scopus.com/inward/record.url?scp=84857557113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857557113&partnerID=8YFLogxK

U2 - 10.1002/jcb.23436

DO - 10.1002/jcb.23436

M3 - Article

C2 - 22065367

AN - SCOPUS:84857557113

VL - 113

SP - 1054

EP - 1060

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 3

ER -