Increased expression of the p27(KIP1) protein in human esophageal cancer cell lines that over-express cyclin D1

Yuichiro Doki, Masaya Imoto, Edward Kyu Ho Han, Alessandro Sgambato, I. Bernard Weinstein

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

In the present study we have characterized eight human esophageal squamous carcinoma cell lines for levels of expression of cyclins D1, E, A and B1; CDKs 1, 2 and 4; the CDK inhibitors p16(INK4), p21(WAF1), and p27(KIP1); the retinoblastoma (Rb) protein; and in vitro CDK2- and CDK4-associated kinase activity; and also compared the growth properties of these cell lines. The level of the cyclin D1 protein varied by over 30-fold amongst the eight cell lines. The high level in two cell lines was associated with amplification of this gene, but in three cell lines it was due to post-transcriptional events. Amongst the eight cell lines there was a significant correlation between the levels of cyclin D1, Rb and p27(KIP1) proteins, and CDK4-associated kinase activity. Furthermore, when an exogenous cyclin D1 cDNA was over-expressed in the EC109 cell line by transfection, this led to increased expression of both Rb and p27(KIP1). There was, however, no correlation between the level of cyclin D1 expression and the cell doubling times, duration of the G1 phase, or colony-forming efficiency in agar. Two of the cell lines displayed a high level of the cyclin E protein, low levels of cyclin D1, lacked expression of the Rb protein and expressed high levels of the p16(INK4) protein. One of these cell lines displayed amplification of the latter gene. There was no correlation between the levels of cyclins E or A and in vitro CDK2 kinase activity, but CDK2 kinase activity was inversely correlated with the duration of the G1 phase of the cell cycle. Taken together, these studies indicate marked heterogeneity in the expression of cell cycle-related proteins amongst a series of esophageal carcinoma cell lines. The correlation between the levels of the cyclin D1, Rb and p27(Kip1) proteins suggest the existence of a homeostatic feedback loop between positive and negative acting components of the cell cycle machinery.

Original languageEnglish
Pages (from-to)1139-1148
Number of pages10
JournalCarcinogenesis
Volume18
Issue number6
DOIs
Publication statusPublished - 1997 Jun
Externally publishedYes

Fingerprint

Cyclin D1
Esophageal Neoplasms
Cell Line
Cyclin E
Retinoblastoma
Phosphotransferases
Retinoblastoma Protein
Gene Amplification
G1 Phase
Cell Cycle
Proteins
human CDKN1B protein
Cyclin-Dependent Kinase Inhibitor p27
Cyclin B1
Cyclin A
Cell Cycle Proteins
Agar
Transfection
Squamous Cell Carcinoma
Complementary DNA

ASJC Scopus subject areas

  • Cancer Research

Cite this

Increased expression of the p27(KIP1) protein in human esophageal cancer cell lines that over-express cyclin D1. / Doki, Yuichiro; Imoto, Masaya; Han, Edward Kyu Ho; Sgambato, Alessandro; Weinstein, I. Bernard.

In: Carcinogenesis, Vol. 18, No. 6, 06.1997, p. 1139-1148.

Research output: Contribution to journalArticle

Doki, Yuichiro ; Imoto, Masaya ; Han, Edward Kyu Ho ; Sgambato, Alessandro ; Weinstein, I. Bernard. / Increased expression of the p27(KIP1) protein in human esophageal cancer cell lines that over-express cyclin D1. In: Carcinogenesis. 1997 ; Vol. 18, No. 6. pp. 1139-1148.
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abstract = "In the present study we have characterized eight human esophageal squamous carcinoma cell lines for levels of expression of cyclins D1, E, A and B1; CDKs 1, 2 and 4; the CDK inhibitors p16(INK4), p21(WAF1), and p27(KIP1); the retinoblastoma (Rb) protein; and in vitro CDK2- and CDK4-associated kinase activity; and also compared the growth properties of these cell lines. The level of the cyclin D1 protein varied by over 30-fold amongst the eight cell lines. The high level in two cell lines was associated with amplification of this gene, but in three cell lines it was due to post-transcriptional events. Amongst the eight cell lines there was a significant correlation between the levels of cyclin D1, Rb and p27(KIP1) proteins, and CDK4-associated kinase activity. Furthermore, when an exogenous cyclin D1 cDNA was over-expressed in the EC109 cell line by transfection, this led to increased expression of both Rb and p27(KIP1). There was, however, no correlation between the level of cyclin D1 expression and the cell doubling times, duration of the G1 phase, or colony-forming efficiency in agar. Two of the cell lines displayed a high level of the cyclin E protein, low levels of cyclin D1, lacked expression of the Rb protein and expressed high levels of the p16(INK4) protein. One of these cell lines displayed amplification of the latter gene. There was no correlation between the levels of cyclins E or A and in vitro CDK2 kinase activity, but CDK2 kinase activity was inversely correlated with the duration of the G1 phase of the cell cycle. Taken together, these studies indicate marked heterogeneity in the expression of cell cycle-related proteins amongst a series of esophageal carcinoma cell lines. The correlation between the levels of the cyclin D1, Rb and p27(Kip1) proteins suggest the existence of a homeostatic feedback loop between positive and negative acting components of the cell cycle machinery.",
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AU - Weinstein, I. Bernard

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