TY - JOUR
T1 - Increased gene expression of matrix metalloproteinase-3 (stromelysin) in skin fibroblasts from patients with severe recessive dystrophic epidermolysis bullosa
AU - Sawamura, Daisuke
AU - Sugawara, Takamitsu
AU - Hashimoto, Isao
AU - Bruckner-Tuderman, Leena
AU - Fujimoto, Daisaburo
AU - Okada, Yasunori
AU - Utsumi, Nobuo
AU - Shikata, Hideo
N1 - Funding Information:
ACKNOWLEDGMENTS: We would like to thank Dr. Zena Werb, University of California, Francisco, Dr. Larry Kedes, Stanford University, and Dr. Darwin J. Prockop, Thomas Jefferson University, for providing us with cDNAs for stromelysin, ,B-actin, and procollagen al(l) chain, respectively. We are also grateful to Dr. Anne Olsen for useful suggestions, and Dr. Helena Kuivaniemi and Dr. Tromp, Department of Biochemistry and Molecular Biology, Thomas Jefferson for their critical reading the manuscript. This work was supported from the Rare and Intractable Dermatoses Research Committee, Japanese Welfare.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1991/1/31
Y1 - 1991/1/31
N2 - Gene expression of matrix metalloproteinase 3 (MMP-3=stromelysin) was examined in the skin fibroblasts obtained from patients with severe recessive dystrophic epidermolysis bullosa (RDEB). Steady-state mRNA level of MMP-3 was selectively increased in the unstimulated RDEB cells by a post-transcriptional mechanism. A parallel study on the susceptibility of type VII collagen to MMPs revealed that this type of collagen is degraded by MMP-3, but not by MMP-1(collagenase). These data suggest that MMP-3 may play an important role in the blister formation of the skin in RDEB patients by the degradation of anchoring fibrils consisting of type VII collagen.
AB - Gene expression of matrix metalloproteinase 3 (MMP-3=stromelysin) was examined in the skin fibroblasts obtained from patients with severe recessive dystrophic epidermolysis bullosa (RDEB). Steady-state mRNA level of MMP-3 was selectively increased in the unstimulated RDEB cells by a post-transcriptional mechanism. A parallel study on the susceptibility of type VII collagen to MMPs revealed that this type of collagen is degraded by MMP-3, but not by MMP-1(collagenase). These data suggest that MMP-3 may play an important role in the blister formation of the skin in RDEB patients by the degradation of anchoring fibrils consisting of type VII collagen.
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U2 - 10.1016/0006-291X(91)91518-H
DO - 10.1016/0006-291X(91)91518-H
M3 - Article
C2 - 1704217
AN - SCOPUS:0026013309
VL - 174
SP - 1003
EP - 1008
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -