Increased gene expression of matrix metalloproteinase-3 (stromelysin) in skin fibroblasts from patients with severe recessive dystrophic epidermolysis bullosa

Daisuke Sawamura; Takamitsu Sugawara; Isao Hashimoto; Leena Bruckner-Tuderman; Daisaburo Fujimoto; Yasunori Okada; Nobuo Utsumi; Hideo Shikata

doi:10.1016/0006-291X(91)91518-H

Increased gene expression of matrix metalloproteinase-3 (stromelysin) in skin fibroblasts from patients with severe recessive dystrophic epidermolysis bullosa

Daisuke Sawamura, Takamitsu Sugawara, Isao Hashimoto, Leena Bruckner-Tuderman, Daisaburo Fujimoto, Yasunori Okada, Nobuo Utsumi, Hideo Shikata

Department of Pathology

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25 Citations (Scopus)

Abstract

Gene expression of matrix metalloproteinase 3 (MMP-3=stromelysin) was examined in the skin fibroblasts obtained from patients with severe recessive dystrophic epidermolysis bullosa (RDEB). Steady-state mRNA level of MMP-3 was selectively increased in the unstimulated RDEB cells by a post-transcriptional mechanism. A parallel study on the susceptibility of type VII collagen to MMPs revealed that this type of collagen is degraded by MMP-3, but not by MMP-1(collagenase). These data suggest that MMP-3 may play an important role in the blister formation of the skin in RDEB patients by the degradation of anchoring fibrils consisting of type VII collagen.

Original language	English
Pages (from-to)	1003-1008
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	174
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(91)91518-H
Publication status	Published - 1991 Jan 31

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Sawamura, D., Sugawara, T., Hashimoto, I., Bruckner-Tuderman, L., Fujimoto, D., Okada, Y., Utsumi, N., & Shikata, H. (1991). Increased gene expression of matrix metalloproteinase-3 (stromelysin) in skin fibroblasts from patients with severe recessive dystrophic epidermolysis bullosa. Biochemical and Biophysical Research Communications, 174(2), 1003-1008. https://doi.org/10.1016/0006-291X(91)91518-H

Increased gene expression of matrix metalloproteinase-3 (stromelysin) in skin fibroblasts from patients with severe recessive dystrophic epidermolysis bullosa. / Sawamura, Daisuke; Sugawara, Takamitsu; Hashimoto, Isao; Bruckner-Tuderman, Leena; Fujimoto, Daisaburo; Okada, Yasunori; Utsumi, Nobuo; Shikata, Hideo.

In: Biochemical and Biophysical Research Communications, Vol. 174, No. 2, 31.01.1991, p. 1003-1008.

Research output: Contribution to journal › Article › peer-review

Sawamura, D, Sugawara, T, Hashimoto, I, Bruckner-Tuderman, L, Fujimoto, D, Okada, Y, Utsumi, N & Shikata, H 1991, 'Increased gene expression of matrix metalloproteinase-3 (stromelysin) in skin fibroblasts from patients with severe recessive dystrophic epidermolysis bullosa', Biochemical and Biophysical Research Communications, vol. 174, no. 2, pp. 1003-1008. https://doi.org/10.1016/0006-291X(91)91518-H

Sawamura D, Sugawara T, Hashimoto I, Bruckner-Tuderman L, Fujimoto D, Okada Y et al. Increased gene expression of matrix metalloproteinase-3 (stromelysin) in skin fibroblasts from patients with severe recessive dystrophic epidermolysis bullosa. Biochemical and Biophysical Research Communications. 1991 Jan 31;174(2):1003-1008. https://doi.org/10.1016/0006-291X(91)91518-H

Sawamura, Daisuke ; Sugawara, Takamitsu ; Hashimoto, Isao ; Bruckner-Tuderman, Leena ; Fujimoto, Daisaburo ; Okada, Yasunori ; Utsumi, Nobuo ; Shikata, Hideo. / Increased gene expression of matrix metalloproteinase-3 (stromelysin) in skin fibroblasts from patients with severe recessive dystrophic epidermolysis bullosa. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 174, No. 2. pp. 1003-1008.

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abstract = "Gene expression of matrix metalloproteinase 3 (MMP-3=stromelysin) was examined in the skin fibroblasts obtained from patients with severe recessive dystrophic epidermolysis bullosa (RDEB). Steady-state mRNA level of MMP-3 was selectively increased in the unstimulated RDEB cells by a post-transcriptional mechanism. A parallel study on the susceptibility of type VII collagen to MMPs revealed that this type of collagen is degraded by MMP-3, but not by MMP-1(collagenase). These data suggest that MMP-3 may play an important role in the blister formation of the skin in RDEB patients by the degradation of anchoring fibrils consisting of type VII collagen.",

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Increased gene expression of matrix metalloproteinase-3 (stromelysin) in skin fibroblasts from patients with severe recessive dystrophic epidermolysis bullosa

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