Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps

Tomoo Sawabe, Yohei Yamazaki, Pedro Milet Meirelles, Sayaka Mino, Wataru Suda, Kenshiro Oshima, Masahira Hattori, Fabiano L. Thompson, Yuichi Sakai, Toko Sawabe

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Gut microbiome shapes various aspects of a host's physiology, but these functions in aquatic animal hosts have yet to be fully investigated. The sea cucumber Apostichopus japonicus Selenka is one such example. The large growth gap in their body size has delayed the development of intensive aquaculture, nevertheless the species is in urgent need of conservation. To understand possible contributions of the gut microbiome to its host's growth, individual fecal microbiome comparisons were performed. High-throughput 16S rRNA sequencing revealed significantly different microbiota in larger and smaller individuals; Rhodobacterales in particular was the most significantly abundant bacterial group in the larger specimens. Further shotgun metagenome of representative samples revealed a significant abundance of microbiome retaining polyhydroxybutyrate (PHB) metabolism genes in the largest individual. The PHB metabolism reads were potentially derived from Rhodobacterales. These results imply a possible link between microbial PHB producers and potential growth promotion in Deuterostomia marine invertebrates.

Original languageEnglish
Article number21631
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 Feb 24

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polyhydroxybutyrate
Apostichopus japonicus
Rhodobacterales
digestive system
metabolism
Holothuroidea
growth promotion
aquaculture
physiology
body size
invertebrates
microbiome
ribosomal RNA
animals
genes

ASJC Scopus subject areas

  • General

Cite this

Sawabe, T., Yamazaki, Y., Meirelles, P. M., Mino, S., Suda, W., Oshima, K., ... Sawabe, T. (2016). Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps. Scientific Reports, 6, [21631]. https://doi.org/10.1038/srep21631

Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps. / Sawabe, Tomoo; Yamazaki, Yohei; Meirelles, Pedro Milet; Mino, Sayaka; Suda, Wataru; Oshima, Kenshiro; Hattori, Masahira; Thompson, Fabiano L.; Sakai, Yuichi; Sawabe, Toko.

In: Scientific Reports, Vol. 6, 21631, 24.02.2016.

Research output: Contribution to journalArticle

Sawabe, T, Yamazaki, Y, Meirelles, PM, Mino, S, Suda, W, Oshima, K, Hattori, M, Thompson, FL, Sakai, Y & Sawabe, T 2016, 'Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps', Scientific Reports, vol. 6, 21631. https://doi.org/10.1038/srep21631
Sawabe, Tomoo ; Yamazaki, Yohei ; Meirelles, Pedro Milet ; Mino, Sayaka ; Suda, Wataru ; Oshima, Kenshiro ; Hattori, Masahira ; Thompson, Fabiano L. ; Sakai, Yuichi ; Sawabe, Toko. / Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps. In: Scientific Reports. 2016 ; Vol. 6.
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