Indocyanine green fluorescence imaging for evaluation of uterine blood flow in cynomolgus macaque

Iori Kisu, Kouji Banno, Makoto Mihara, Li Yu Lin, Kosuke Tsuji, Megumi Yanokura, Hisako Hara, Jun Araki, Takuya Iida, Takayuki Abe, Keisuke Koyama, Nobuhiko Suganuma, Daisuke Aoki

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: Uterine blood flow is an important factor in uterine viability, but the number of blood vessels required to maintain viability is uncertain. In this study, indocyanine green (ICG) fluorescence imaging was used to examine uterine hemodynamics and vessels associated with uterine blood flow in cynomolgus macaque. Methods: The uterus of a female cynomolgus macaque was cut from the vaginal canal to mimic a situation during trachelectomy or uterine transplantation surgery in which uterine perfusion is maintained only with uterine and ovarian vessels. Intraoperative uterine hemodynamics was observed using ICG fluorescence imaging under conditions in which various nutrient vessels were selected by clamping of blood vessels. A time-intensity curve was plotted using imaging analysis software to measure the T max of uterine perfusion for selected blood vessel patterns. Open surgery was performed with the uterus receiving nutritional support only from uterine vessels on one side. The size of the uterus after surgery was monitored using transabdominal ultrasonography. Results: The resulting time-intensity curves displayed the average intensity in the regions of the uterine corpus and uterine cervix, and in the entire uterus. Analyses of the uterine hemodynamics in the cynomolgus macaque showed that uterine vessels were significantly related to uterine perfusion (P = 0.008), whereas ovarian vessels did not have a significant relationship (P = 0.588). When uterine vessels were clamped, ovarian vessels prolonged the time needed to reach perfusion maximum. Postoperative transabdominal ultrasonography showed that the uterus atrophied over time, without recovery of menstrual discharge. Conclusion: Uterine vessels may be responsible for uterine blood flow, but one uterine vessel may be insufficient to maintain uterine viability in cynomolgus macaque. Our results show that ICG fluorescence imaging is useful for evaluation of uterine blood flow since this method allows real-time observation of uterine hemodynamics.

Original languageEnglish
Article numbere35124
JournalPLoS One
Volume7
Issue number4
DOIs
Publication statusPublished - 2012 Apr 20

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Indocyanine Green
Macaca fascicularis
Optical Imaging
Hemodynamics
Macaca
uterus
blood flow
Uterus
hemodynamics
Blood vessels
Blood
Fluorescence
image analysis
fluorescence
Surgery
Ultrasonography
Imaging techniques
Perfusion
blood vessels
Blood Vessels

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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Indocyanine green fluorescence imaging for evaluation of uterine blood flow in cynomolgus macaque. / Kisu, Iori; Banno, Kouji; Mihara, Makoto; Lin, Li Yu; Tsuji, Kosuke; Yanokura, Megumi; Hara, Hisako; Araki, Jun; Iida, Takuya; Abe, Takayuki; Koyama, Keisuke; Suganuma, Nobuhiko; Aoki, Daisuke.

In: PLoS One, Vol. 7, No. 4, e35124, 20.04.2012.

Research output: Contribution to journalArticle

Kisu, Iori ; Banno, Kouji ; Mihara, Makoto ; Lin, Li Yu ; Tsuji, Kosuke ; Yanokura, Megumi ; Hara, Hisako ; Araki, Jun ; Iida, Takuya ; Abe, Takayuki ; Koyama, Keisuke ; Suganuma, Nobuhiko ; Aoki, Daisuke. / Indocyanine green fluorescence imaging for evaluation of uterine blood flow in cynomolgus macaque. In: PLoS One. 2012 ; Vol. 7, No. 4.
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