Indocyanine green injection for detecting sentinel nodes using color fluorescence camera in the laparoscopy-assisted gastrectomy

Masashi Yoshida, Keisuke Kubota, Junko Kuroda, Keiichiro Ohta, Tetsuya Nakamura, Junichi Saito, Michiya Kobayashi, Takayuki Sato, Yoshifumi Beck, Yuukou Kitagawa, Masaki Kitajima

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Background and Aim: We seek for the accurate and simple method for detecting sentinel nodes of gastric cancer which can be popularized in community hospitals. The indocyanine green (ICG) fluorescence-guided method is reported to be sensitive. However, the ordinal fluorescence cameras have gray scale imaging and require a dark room. We have developed a new device, Hyper Eye Medical System (HEMS) which can simultaneously detect color and near-infrared rays and can be used under room light. This study was planned to examine whether submucosal injection of 0.5mL×4 of 50μg/mL ICG on the day before operation is the adequate administration for detecting sentinel nodes using HEMS in the gastric cancer surgery. Methods: The patients underwent gastrectomy for clinical T1a (mucosa)-T2 (muscularis propria) and clinical N0 were enrolled in the present study. As a preliminary trial, one case each of the ICG 25 and 100μg/mL, injected on the day before operation and intraoperative injection, was examined. Then, 10 cases injected 50μg/mL ICG on the day before operation were examined. Results: The ICG fluorescence of the patient injected 100μg/mL was too intense and that of the patient injected 25μg/mL was too faint. Sentinel lymph nodes were detected in all of 10 cases injected 50μg/mL, the day before operation and number of sentinel lymph nodes per patient was 3.6±2.1. Metastasis was observed in one case. All of ICG fluorescence-positive sentinel nodes were positive for the metastasis. In the patient who underwent intraoperative injection, sentinel lymphatic basins could be identified. Conclusion: The present study shows that HEMS-guided abdominal surgery is feasible under room light. Submucosal injection of 0.5mL×4 of 50μg/mL ICG on the day before operation is the adequate administration for detecting sentinel nodes using HEMS in the gastric cancer surgery.

Original languageEnglish
Pages (from-to)29-33
Number of pages5
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume27
Issue numberSUPPL.3
DOIs
Publication statusPublished - 2012

Fingerprint

Indocyanine Green
Gastrectomy
Laparoscopy
Color
Fluorescence
Injections
Stomach Neoplasms
Infrared Rays
Neoplasm Metastasis
Light
Community Hospital
cyhalothrin
Mucous Membrane
Equipment and Supplies

Keywords

  • Fluorescence
  • Gastric cancer
  • Hyper Eye Medical System
  • Indocyanine green
  • Sentinel nodes

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Indocyanine green injection for detecting sentinel nodes using color fluorescence camera in the laparoscopy-assisted gastrectomy. / Yoshida, Masashi; Kubota, Keisuke; Kuroda, Junko; Ohta, Keiichiro; Nakamura, Tetsuya; Saito, Junichi; Kobayashi, Michiya; Sato, Takayuki; Beck, Yoshifumi; Kitagawa, Yuukou; Kitajima, Masaki.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 27, No. SUPPL.3, 2012, p. 29-33.

Research output: Contribution to journalArticle

Yoshida, Masashi ; Kubota, Keisuke ; Kuroda, Junko ; Ohta, Keiichiro ; Nakamura, Tetsuya ; Saito, Junichi ; Kobayashi, Michiya ; Sato, Takayuki ; Beck, Yoshifumi ; Kitagawa, Yuukou ; Kitajima, Masaki. / Indocyanine green injection for detecting sentinel nodes using color fluorescence camera in the laparoscopy-assisted gastrectomy. In: Journal of Gastroenterology and Hepatology (Australia). 2012 ; Vol. 27, No. SUPPL.3. pp. 29-33.
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AU - Yoshida, Masashi

AU - Kubota, Keisuke

AU - Kuroda, Junko

AU - Ohta, Keiichiro

AU - Nakamura, Tetsuya

AU - Saito, Junichi

AU - Kobayashi, Michiya

AU - Sato, Takayuki

AU - Beck, Yoshifumi

AU - Kitagawa, Yuukou

AU - Kitajima, Masaki

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KW - Sentinel nodes

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